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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria Tropical; Embrapa Algodão. |
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Data corrente: |
06/04/1994 |
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Data da última atualização: |
06/04/1994 |
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Autoria: |
LITZ, R. E.; KNIGHT JR, R. J.; GAZIT, S. |
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Título: |
In vitro somatic embryogenesis from mangifera indica L. callus. |
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Ano de publicação: |
1984 |
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Fonte/Imprenta: |
Scientia Horticulturae, v.22, n.3, p.233-240, 1984. |
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Idioma: |
Inglês |
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Conteúdo: |
The nucellus and globular adventitious proembryos were removed from 2-month-old fruits of mango (Mangifera indica L.) cultivars `Ono' and `Chino', and were cultured on sterile, solid Murashige and Skoog (MS) medium that had been modified as follows: half-strength major salts and chelated iron; 20% (v/v) coconut water (CW); 6% sucrose; 100 mg l-1 ascorbic acid and 400 mg l-1 gultamine. Embriogenic explants were sub-cultured after 4-6 weeks in liquid modified MS medium containing 2 mg l-1 2,4-dichlorophenoxyacetic acid (2,4-D) instead of CW. Rapidly growing cultures were established and were sub-cultured monthly. Somatic embyogenesis was induced following sub-culture from MS medium with 2,4-D to MS without growth regulators and with or without activated charcoal (0.5%). Germination of somatic embryos appeared to be enhanced by 1 mg l-1 benzyladenine (BA); however, most of the germinating embryos became embryogenetic. |
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Palavras-Chave: |
Embrigênese somática; Inglês - mango; Manga - cultura de tecidos; Manga - embriogenese somatica; Mangueira; Português-manga. |
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Thesagro: |
Cultura de Tecido; Manga; Mangifera Indica. |
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Thesaurus Nal: |
somatic embryogenesis; tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01700naa a2200277 a 4500
001 1417979
005 1994-04-06
008 1984 bl --- 0-- u #d
100 1 $aLITZ, R. E.
245 $aIn vitro somatic embryogenesis from mangifera indica L. callus.
260 $c1984
520 $aThe nucellus and globular adventitious proembryos were removed from 2-month-old fruits of mango (Mangifera indica L.) cultivars `Ono' and `Chino', and were cultured on sterile, solid Murashige and Skoog (MS) medium that had been modified as follows: half-strength major salts and chelated iron; 20% (v/v) coconut water (CW); 6% sucrose; 100 mg l-1 ascorbic acid and 400 mg l-1 gultamine. Embriogenic explants were sub-cultured after 4-6 weeks in liquid modified MS medium containing 2 mg l-1 2,4-dichlorophenoxyacetic acid (2,4-D) instead of CW. Rapidly growing cultures were established and were sub-cultured monthly. Somatic embyogenesis was induced following sub-culture from MS medium with 2,4-D to MS without growth regulators and with or without activated charcoal (0.5%). Germination of somatic embryos appeared to be enhanced by 1 mg l-1 benzyladenine (BA); however, most of the germinating embryos became embryogenetic.
650 $asomatic embryogenesis
650 $atissue culture
650 $aCultura de Tecido
650 $aManga
650 $aMangifera Indica
653 $aEmbrigênese somática
653 $aInglês - mango
653 $aManga - cultura de tecidos
653 $aManga - embriogenese somatica
653 $aMangueira
653 $aPortuguês-manga
700 1 $aKNIGHT JR, R. J.
700 1 $aGAZIT, S.
773 $tScientia Horticulturae$gv.22, n.3, p.233-240, 1984.
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Registro original: |
Embrapa Agroindústria Tropical (CNPAT) |
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