Data/hora: 01/06/2024 22:19:28

Biblioteca(s): | Embrapa Agroindústria Tropical; Embrapa Algodão.
Data corrente: | 06/04/1994
Data da última atualização: | 06/04/1994
Autoria: | LITZ, R. E.; KNIGHT JR, R. J.; GAZIT, S.
Título: | In vitro somatic embryogenesis from mangifera indica L. callus.
Ano de publicação: | 1984
Fonte/Imprenta: | Scientia Horticulturae, v.22, n.3, p.233-240, 1984.
Idioma: | Inglês
Conteúdo: | The nucellus and globular adventitious proembryos were removed from 2-month-old fruits of mango (Mangifera indica L.) cultivars `Ono' and `Chino', and were cultured on sterile, solid Murashige and Skoog (MS) medium that had been modified as follows: half-strength major salts and chelated iron; 20% (v/v) coconut water (CW); 6% sucrose; 100 mg l-1 ascorbic acid and 400 mg l-1 gultamine. Embriogenic explants were sub-cultured after 4-6 weeks in liquid modified MS medium containing 2 mg l-1 2,4-dichlorophenoxyacetic acid (2,4-D) instead of CW. Rapidly growing cultures were established and were sub-cultured monthly. Somatic embyogenesis was induced following sub-culture from MS medium with 2,4-D to MS without growth regulators and with or without activated charcoal (0.5%). Germination of somatic embryos appeared to be enhanced by 1 mg l-1 benzyladenine (BA); however, most of the germinating embryos became embryogenetic.
Palavras-Chave: | Embrigênese somática; Inglês - mango; Manga - cultura de tecidos; Manga - embriogenese somatica; Mangueira; Português-manga.
Thesagro: | Cultura de Tecido; Manga; Mangifera Indica.
Thesaurus Nal: | somatic embryogenesis; tissue culture.
Categoria do assunto: | --
Registro original: | Embrapa Agroindústria Tropical (CNPAT)

Exemplares:

CNPA - (17526-1) - ADD - SP - -- - S 6051 -  - 98-0047 - DIS
CNPAT - (960-1) - EMB - SP - -- - SP 01431 -  - 94.01431 - DIS