Data/hora: 08/06/2024 20:56:04

Biblioteca(s): 	Embrapa Meio Ambiente.	
Data corrente: 	10/12/2019	
Data da última atualização: 	09/08/2021	
Autoria: 	LIRA, A. C. de; MASCARIN, G. M.; DELALIBERA JUNIOR, I.	
Afiliação: 	A. C. de LIRA; GABRIEL MOURA MASCARIN, CNPMA; I. DELALIBERA JUNIOR.	
Título: 	Maize seed coating with microsclerotia of Metarhizium spp. as biostimulant and protection against Spodoptera frugiperda.	
Ano de publicação: 	2019	
Fonte/Imprenta: 	In: SIMPÓSIO DE CONTROLE BIOLÓGICO (SICONBIOL), 16., 2019, Londrina. Anais... Londrina: SEB; UEL; Embrapa, 2019.	
Páginas: 	p. 55.	
Idioma: 	Português	
Conteúdo: 	The use of microsclerotia (MS) of the entomopathogenic fungi Metarhiziumspp. may be an alternative for pest control due to its long persistence in soil and production of infective conidia in situ. In this study we produce MS of 48 isolates of Metarhiziumspp. and selected three most productive isolates to be used in maize seed coating for two purposes: enhancement of plants growth and protection against Spodoptera frugiperda(Lepidoptera: Noctuidae). MS were cultured in liquid medium with carbon:nitrogen (C:N) ratio of 10:1. The role of Metarhiziumas a biostimulant was measured on 30-day-old plants grown from seeds inoculated with microsclerotia of the three selected isolates. Survival of S. frugiperda3rdinstarlarvae was evaluated on maize plants grown from MS-coated seeds. MS yields were 1.2×103, 1.8×103 and 3.6×103 MS mL-1 within 4 days of fermentation for M. anisopliaeESALQ1814, M. robertsiiESALQ2450 and Metarhiziumsp. indeterminate ESALQ1638, respectively. The germination rates of MS for these isolates were always above 90% in 24h incubation period, while conidia concentration from sporogenic MS after 7 days incubation on water agar reached 1.33×108 -1.31×109 conidia g-1 of MS granules. The isolates of MetarhiziumESALQ1638 improved somehow maize plant growth; higher shoot height and root size, while increased plant biomass was shown with ESALQ1814 and ESALQ2450. Mortality of S. frugiperdawas 55-64% and median survival time (ST50) was 5-6 days when larvae were fed on maize plant inoculated with any of the three Metarhiziumspecies. Due to the lack of mycosis on larvae by Metarhiziumspp., we postulate that the indirect detrimental effect on S. frugiperdasurvival was probably linked with plant biochemicaldefenses. Our results prompt the feasibility and effectiveness of using MS of Metarhiziumspp. in maize seed coating with a dual benefit in plant growth promotion and protection against insect feeding.	
Palavras-Chave: 	Fungal entomopathogen; Liquid fermentation; Plant biostimulant.	
Thesaurus Nal: 	Metarhizium.	
Categoria do assunto: 	H Saúde e Patologia	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/206703/1/RA-MascarinGM-16SICONBIOL-2019-p55.pdf	
Registro original: 	Embrapa Meio Ambiente (CNPMA)	
Exemplares: 	CNPMA - (16611-1) - UPC - RA - DD -  -  -  - DIS