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Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
06/07/2017 |
Data da última atualização: |
06/07/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
TEIXEIRA, F. B.; SILVA, R. de B.; LAMEIRA, O. A.; WEBBER, L. P.; COUTO, R. S. D.; MARTINS, M. D.; LIMA, R. R. |
Afiliação: |
Francisco Bruno Teixeira, UFPA; Raíra de Brito Silva, UFPA; OSMAR ALVES LAMEIRA, CPATU; Liana Preto Webber, UFRGS; Roberta Souza D'Almeida Couto, UFPA; Manoela Domingues Martins, UFRGS; Rafael Rodrigues Lima, UFPA. |
Título: |
Copaiba oil-resin (Copaifera reticulata Ducke) modulates the inflammation in a model of injury to rats' tongues. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
BMC Complementary and Alternative Medicine, v. 17, n. 1, article 313, June 2017. |
DOI: |
10.1186/s12906-017-1820-2 |
Idioma: |
Inglês |
Conteúdo: |
A method for the separation, identification and quantification of 24 phenolic compounds using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was developed and validated. Six species of traditional medicinal plants from the Brazilian Amazon region were studied (Mansoa alliacea, Bauhinia variegata var. variegata, Bauhinia variegata var. alboflava, Connarus perrottetii var. angustifolius, Cecropia obtusa and Cecropia palmata). The analytes were separated by a reversed-phase SB-C18 column (2.1 × 50 mm, 1.8 μm) using a gradient elution of 7 min composed of 0.1% acetic acid in water (v/v) and acetonitrile, at a constant flow rate of 0.8 mL min−1. The limit of detection for the analytes ranged between 0.5 and 130.3 μg L−1. Intra- and inter-day precision showed satisfactory results and the recoveries obtained for the 24 analytes varied between 91.7 and 111.9% for most of the evaluated matrices. Quantifications were performed with the standard addition method (r > 0.99) using the data acquired in a multiple reaction monitoring (MRM) mode. Rutin, apigenin, vanillic acid, caffeic acid, ferulic acid, p-coumaric acid and trans-cinnamic acid were found in all the studied plants. However, we highlight the high contents of rutin in varieties of Bauhinia variegata (up to 2.52 mg g−1 of plant), chlorogenic acid in species of genus Cecropia (up to 0.57 mg g−1 of plant) and catechin in Connarus perrottetii var. angustifolius (1.77 mg g−1 of plant). MenosA method for the separation, identification and quantification of 24 phenolic compounds using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was developed and validated. Six species of traditional medicinal plants from the Brazilian Amazon region were studied (Mansoa alliacea, Bauhinia variegata var. variegata, Bauhinia variegata var. alboflava, Connarus perrottetii var. angustifolius, Cecropia obtusa and Cecropia palmata). The analytes were separated by a reversed-phase SB-C18 column (2.1 × 50 mm, 1.8 μm) using a gradient elution of 7 min composed of 0.1% acetic acid in water (v/v) and acetonitrile, at a constant flow rate of 0.8 mL min−1. The limit of detection for the analytes ranged between 0.5 and 130.3 μg L−1. Intra- and inter-day precision showed satisfactory results and the recoveries obtained for the 24 analytes varied between 91.7 and 111.9% for most of the evaluated matrices. Quantifications were performed with the standard addition method (r > 0.99) using the data acquired in a multiple reaction monitoring (MRM) mode. Rutin, apigenin, vanillic acid, caffeic acid, ferulic acid, p-coumaric acid and trans-cinnamic acid were found in all the studied plants. However, we highlight the high contents of rutin in varieties of Bauhinia variegata (up to 2.52 mg g−1 of plant), chlorogenic acid in species of genus Cecropia (up to 0.57 mg g−1 of plant) and catechin in Connarus perrottetii var. an... Mostrar Tudo |
Palavras-Chave: |
Copaifera Reticulata Ducke. |
Thesagro: |
Copaíba; Planta Medicinal. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161429/1/12906-2017-Article-1820.pdf
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Marc: |
LEADER 02316naa a2200241 a 4500 001 2072092 005 2017-07-06 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1186/s12906-017-1820-2$2DOI 100 1 $aTEIXEIRA, F. B. 245 $aCopaiba oil-resin (Copaifera reticulata Ducke) modulates the inflammation in a model of injury to rats' tongues.$h[electronic resource] 260 $c2017 520 $aA method for the separation, identification and quantification of 24 phenolic compounds using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) was developed and validated. Six species of traditional medicinal plants from the Brazilian Amazon region were studied (Mansoa alliacea, Bauhinia variegata var. variegata, Bauhinia variegata var. alboflava, Connarus perrottetii var. angustifolius, Cecropia obtusa and Cecropia palmata). The analytes were separated by a reversed-phase SB-C18 column (2.1 × 50 mm, 1.8 μm) using a gradient elution of 7 min composed of 0.1% acetic acid in water (v/v) and acetonitrile, at a constant flow rate of 0.8 mL min−1. The limit of detection for the analytes ranged between 0.5 and 130.3 μg L−1. Intra- and inter-day precision showed satisfactory results and the recoveries obtained for the 24 analytes varied between 91.7 and 111.9% for most of the evaluated matrices. Quantifications were performed with the standard addition method (r > 0.99) using the data acquired in a multiple reaction monitoring (MRM) mode. Rutin, apigenin, vanillic acid, caffeic acid, ferulic acid, p-coumaric acid and trans-cinnamic acid were found in all the studied plants. However, we highlight the high contents of rutin in varieties of Bauhinia variegata (up to 2.52 mg g−1 of plant), chlorogenic acid in species of genus Cecropia (up to 0.57 mg g−1 of plant) and catechin in Connarus perrottetii var. angustifolius (1.77 mg g−1 of plant). 650 $aCopaíba 650 $aPlanta Medicinal 653 $aCopaifera Reticulata Ducke 700 1 $aSILVA, R. de B. 700 1 $aLAMEIRA, O. A. 700 1 $aWEBBER, L. P. 700 1 $aCOUTO, R. S. D. 700 1 $aMARTINS, M. D. 700 1 $aLIMA, R. R. 773 $tBMC Complementary and Alternative Medicine$gv. 17, n. 1, article 313, June 2017.
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Embrapa Amazônia Oriental (CPATU) |
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Registros recuperados : 5 | |
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