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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
28/09/2010 |
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Data da última atualização: |
30/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; SOUZA, M. M.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. |
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Afiliação: |
N. Z. SARAIVA, UNESP/JABOTICABAL; C. S. OLIVEIRA, UNESP/JABOTICABAL; T. A. D. TETZNER, UNESP/JABOTICABAL; M. M. SOUZA, UNESP/JABOTICABAL; M. R. DE LIMA, UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; J. M. GARCIA, UNESP/JABOTICABAL. |
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Título: |
Bovine cytoplasts prepared by demecolcine-induced enucleation of activated oocytes. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010; Reproduction, Fertility and development, v. 22, n. 1, 2010. |
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Páginas: |
p. 197 |
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Idioma: |
Português |
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Conteúdo: |
One of the most critical steps of the standard NT procedure is the removal of oocyte chromosomes for the production of enucleated cytoplasts. This process involves ultraviolet (UV) irradiation, which causes damage to the membrane and intracellular components of bovine oocytes. The objective of the present study was to evaluate the use of demecolcine, a microtubule depolymerizing agent, for chemical-induced enucleation of activated bovine oocytes. In the first experiment, oocytes obtained from cow ovaries collected in a slaughterhouse were IVM in TCM-199 medium supplemented with 10% FCS, FSH, hCG, estradiol, pyruvate, and amikacin for 26 h; then, they were artificially activated with 5 ?M ionomycin for 5 min and 10 ?g mL-1 cycloheximide for 4 h. The following groups were established: control, C (no activation and no demecolcine); activated, A (exposure only to activating agents); and demecolcine-exposed oocytes, DEME (activation for 4 h and 0.05 ?g mL-1 demecolcine for 2 to 4 h after 0, 0.5, 1.0, 1.5, and 2.0 h of activation). The treatment DEME comprised the groups G1 (demecolcine from 0 to 2 h of activation; DEME 0-2 h); G2 (DEME 0-4 h); G3 (DEME 0.5-2.5 h); G4 (DEME 0.5-4 h); G5 (DEME 1-3 h); G6 (DEME 1-4 h); G7 (DEME 1.5-3.5 h); G8 (DEME 1.5-4 h); and G9 (DEME 2-4h). The oocytes were stained with 10 ?g mL-1 Hoechst 33342 for 15 min and enucleation rates (EN) were evaluated under epifluorescence microscope (330-385 nm).After EN evaluation, a second experiment was performed to verify the efficiency of demecolcine-induced enucleation in group G9, which showed the best result in the first experiment. After demecolcine treatment, the oocytes were incubated in HEPES-buffered SOF with 10% FCS and cytochalasin B for removal of the 2PB and minimal adjacent cytoplast under an inverted optical microscope. Traditional enucleation was performed on the control group without exposure of the oocytes to demecolcine. The same conditions were employed except that UV light was used to confirm enucleation. Samples of cytoplasts were stained with Hoechst for 15 min and analyzed for enucleation efficiency. Five and 3 replicates were evaluated, respectively, in experiments 1 and 2, and the results were analyzed by chi-square test in the statistical software Minitab®, release 14.1 (Minitab Inc., State College, PA, USA). A level of 5% significance was used. Regarding enucleation rates, all treated groups were significantly different compared with the C (0/114) and A (0/130) groups. Considering the DEME groups, G8 (46/109; 42.2%) and G9 (61/113; 54.0%) presented superior rates (P < 0.05) to all other groups (26.8 to 36.3%), but they were similar despite the great tendency (P = 0.07) to difference. We observed high efficiency (P < 0.05) of demecolcine-induced enucleation (90/110; 81.8%) compared with a traditional technique (61/95; 64.2%). In conclusion, the present study shows that demecolcine-induced enucleation of activated oocytes enables good rates of enucleation and has greater efficiency than the traditional technique as well as avoiding UV irradiation of the cytoplast. MenosOne of the most critical steps of the standard NT procedure is the removal of oocyte chromosomes for the production of enucleated cytoplasts. This process involves ultraviolet (UV) irradiation, which causes damage to the membrane and intracellular components of bovine oocytes. The objective of the present study was to evaluate the use of demecolcine, a microtubule depolymerizing agent, for chemical-induced enucleation of activated bovine oocytes. In the first experiment, oocytes obtained from cow ovaries collected in a slaughterhouse were IVM in TCM-199 medium supplemented with 10% FCS, FSH, hCG, estradiol, pyruvate, and amikacin for 26 h; then, they were artificially activated with 5 ?M ionomycin for 5 min and 10 ?g mL-1 cycloheximide for 4 h. The following groups were established: control, C (no activation and no demecolcine); activated, A (exposure only to activating agents); and demecolcine-exposed oocytes, DEME (activation for 4 h and 0.05 ?g mL-1 demecolcine for 2 to 4 h after 0, 0.5, 1.0, 1.5, and 2.0 h of activation). The treatment DEME comprised the groups G1 (demecolcine from 0 to 2 h of activation; DEME 0-2 h); G2 (DEME 0-4 h); G3 (DEME 0.5-2.5 h); G4 (DEME 0.5-4 h); G5 (DEME 1-3 h); G6 (DEME 1-4 h); G7 (DEME 1.5-3.5 h); G8 (DEME 1.5-4 h); and G9 (DEME 2-4h). The oocytes were stained with 10 ?g mL-1 Hoechst 33342 for 15 min and enucleation rates (EN) were evaluated under epifluorescence microscope (330-385 nm).After EN evaluation, a second experiment was performed... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Cytoplasts. |
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Thesaurus Nal: |
oocytes. |
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Categoria do assunto: |
W Química e Física |
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Marc: |
LEADER 03900nam a2200229 a 4500
001 1863081
005 2023-06-30
008 2010 bl uuuu u00u1 u #d
100 1 $aSARAIVA, N. Z.
245 $aBovine cytoplasts prepared by demecolcine-induced enucleation of activated oocytes.$h[electronic resource]
260 $aIn: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010; Reproduction, Fertility and development, v. 22, n. 1, 2010.$c2010
300 $ap. 197
520 $aOne of the most critical steps of the standard NT procedure is the removal of oocyte chromosomes for the production of enucleated cytoplasts. This process involves ultraviolet (UV) irradiation, which causes damage to the membrane and intracellular components of bovine oocytes. The objective of the present study was to evaluate the use of demecolcine, a microtubule depolymerizing agent, for chemical-induced enucleation of activated bovine oocytes. In the first experiment, oocytes obtained from cow ovaries collected in a slaughterhouse were IVM in TCM-199 medium supplemented with 10% FCS, FSH, hCG, estradiol, pyruvate, and amikacin for 26 h; then, they were artificially activated with 5 ?M ionomycin for 5 min and 10 ?g mL-1 cycloheximide for 4 h. The following groups were established: control, C (no activation and no demecolcine); activated, A (exposure only to activating agents); and demecolcine-exposed oocytes, DEME (activation for 4 h and 0.05 ?g mL-1 demecolcine for 2 to 4 h after 0, 0.5, 1.0, 1.5, and 2.0 h of activation). The treatment DEME comprised the groups G1 (demecolcine from 0 to 2 h of activation; DEME 0-2 h); G2 (DEME 0-4 h); G3 (DEME 0.5-2.5 h); G4 (DEME 0.5-4 h); G5 (DEME 1-3 h); G6 (DEME 1-4 h); G7 (DEME 1.5-3.5 h); G8 (DEME 1.5-4 h); and G9 (DEME 2-4h). The oocytes were stained with 10 ?g mL-1 Hoechst 33342 for 15 min and enucleation rates (EN) were evaluated under epifluorescence microscope (330-385 nm).After EN evaluation, a second experiment was performed to verify the efficiency of demecolcine-induced enucleation in group G9, which showed the best result in the first experiment. After demecolcine treatment, the oocytes were incubated in HEPES-buffered SOF with 10% FCS and cytochalasin B for removal of the 2PB and minimal adjacent cytoplast under an inverted optical microscope. Traditional enucleation was performed on the control group without exposure of the oocytes to demecolcine. The same conditions were employed except that UV light was used to confirm enucleation. Samples of cytoplasts were stained with Hoechst for 15 min and analyzed for enucleation efficiency. Five and 3 replicates were evaluated, respectively, in experiments 1 and 2, and the results were analyzed by chi-square test in the statistical software Minitab®, release 14.1 (Minitab Inc., State College, PA, USA). A level of 5% significance was used. Regarding enucleation rates, all treated groups were significantly different compared with the C (0/114) and A (0/130) groups. Considering the DEME groups, G8 (46/109; 42.2%) and G9 (61/113; 54.0%) presented superior rates (P < 0.05) to all other groups (26.8 to 36.3%), but they were similar despite the great tendency (P = 0.07) to difference. We observed high efficiency (P < 0.05) of demecolcine-induced enucleation (90/110; 81.8%) compared with a traditional technique (61/95; 64.2%). In conclusion, the present study shows that demecolcine-induced enucleation of activated oocytes enables good rates of enucleation and has greater efficiency than the traditional technique as well as avoiding UV irradiation of the cytoplast.
650 $aoocytes
653 $aBovine
653 $aCytoplasts
700 1 $aOLIVEIRA, C. S.
700 1 $aTETZNER, T. A. D.
700 1 $aSOUZA, M. M.
700 1 $aLIMA, M. R. de
700 1 $aNICIURA, S. C. M.
700 1 $aGARCIA, J. M.
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Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 35 | |
| 21. |  | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; LIMA, M. R. de; MELO, D. S. de; NICIURA, S. C. M.; GARCIA, J. M. Chemically assisted enucleation results in higher G6PD expression in early bovine female embryos obtained by somatic cell nuclear transfer. Cellular Reprogramming, v. 14, n. 5, p. 1-11, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
|    |
| 22. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; COLLADO, M. del; LIMA, M. R. de; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. Protocol optimization and evaluation of maturation promoting factor and mitogen-activated protein kina sea ctivities in bovine cytoplasts obtained by chemical enucleation techniques. Reproduction Fertility Devevelopment, v. 26, n. 1, p. 130, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
|  |
| 23. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; COLLADO, M. DEL; LIMA, M. R. DE; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. Protocol optimization and evaluation of maturation promoting factor and mitogen-activated protein kinase activities in bovine cytoplasts obtained by chemical enucleation techniques. Reproduction Fertility and Development, v. 26, n. 1, p. 130, 2014. Suplemento. Proceedings da Annual Conference of the International Embryo Transfer Societty, 2014, Reno.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 24. | | RODRIGUES, V. G. S.; COSTA, R. S. C. da; LEÔNIDAS, F. das C.; SANTOS, M. R. A. dos; LIMA, M. R. de. Efeito da arborização na cobertura do solo em agrossistemas com café robusta (Coffea canephora) em Machadinho Do Oeste-RO. In: REUNIÃO AMAZÔNICA DE AGROECOLOGIA, 1., 2007, Manaus. A agroecologia no contexto amazônico: palestras, relatos de experiência e resumos. Manaus: Embrapa Amazônia Ocidental, 2007. 1 CD ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Rondônia. |
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| 25. | | SARAIVA, N. Z.; OLIVEIRA, C. L.; DEL COLLADO, C. L.; LIMA, M. R. de; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. Effects of trichostatina, a chromatin-modifying agent, on meiotic progression of bovine oocytes and embryonic development. In: CONGRESSO BRASILEIRO BRASILEIRO DE GENÉTICA, 59., 2013, Águas de Lindóia. Resumos... Águas de Lindóia: SBG, 2013. p. 54.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 26. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; DEL COLLADO, M.; LIMA, M. R. DE; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. Effects of Trichostatin A, A chromatin-modifying agent, on meiotic progression of bovine oocutes and embryonic development. In: CONGRESSO BRASILEIRO BRASILEIRO DE GENÉTICA, 59., 2013, Águas de Lindóia. Resumos... Águas de Lindóia: Sociedade Brasileira de Genética, 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 27. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. Effects of demecolcine on the meiotic cell-cycle and microtubular kinetics of activated bovine oocytes submitted to chemical enucleation. In: ANUAL CONFERENCE OF INTERNATIONAL EMBRYO TRANSFER SOCIETY, 24., Phoenix, Arizona. Phoenix: CSIRO, 2012 p. 119| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 28. | | OLIVEIRA, C. S.; SARAIVA, N. Z.; LIMA, M. R. de; OLIVEIRA, L. Z.; SERAPIÃO, R. V.; BORGES, C. A. V.; GARCIA, J. M.; CAMARGO, L. S. de A. Kinetics data from bovine sex-specific embryo development from three different bulls. Data in Brief, v. 7, p. 1211-1216, June 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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| 29. | | TORRES, T. R.; LUDKE, M. do C. M. M.; LUDKE, J. V.; SOUZA, E. J. O. de; SERAFIM, J. E.; LIMA, M. R. de. Milheto em dietas para frangos de corte. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 6., 2010, Mossoró. Anais... Mossoró: SNPA, 2010. 1 CD-ROM. Projeto/Plano de Ação: 16.00.30004-00.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 30. | | TORRES, T. R.; LUDKE, M. do C. M. M.; LUDKE, J. V.; SANTOS, M. J. B. dos; LIMA, M. R. de; SANTOS, P. A. dos. Performance of broilers fed during 21 days on mash or pellet diets containing whole or ground pearl millet grain. Brazilian Journal of Poultry Science, v. 15, n. 4, p. 1-6, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 31. | | FEUCHARD, V. L. da S.; MARQUES, S. C. de S.; SERAPIÃO, R. V.; SARAIVA, N. Z.; LIMA, M. R. de; GARCIA, J. M.; OLIVEIRA, C. S. Use of lipolysis supplement during IVM reduces lipid content of bovine oocytes but do not affect blastocyst cryosurvival. In: Annual Meeting of the Braziliam Embrio Technology Society, 33rd. Resumo em Anais. Ilha de Comandatuba, Bahia. SBTE, 2019. p 644.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 32. | | COSTA, A. A. G.; LUDKE, M. do C. M. M.; LUDKE, J. V.; HOLANDA, M. A. C. de; OLIVEIRA, E. L. de O; LIMA, M. R. de. Viabilidade econômica do uso de farinha de penas hidrolisadas na ração de frango de corte macho In: JORNADA DE ENSINO, PESQUISA E EXTENSÃO DA UFRPE, 9; SEMANA NACIONAL DE CIÊNCIA E TECNOLOGIA, 6; 2009, Recife. Anais.. Recife: UFRPE, 2009. 1 CD-ROM. Projeto/Plano de Ação: 16.00.30004-00| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 33. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; LEAL, C. L. V.; LIMA, M. R. de; CALLADO, M. del; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. Chemically induced enucleation of activated bovine oocytes: chromatin and microtubule organization and production of viable cytoplasts. Zygote, v. 23, n. 6, p. 852-862, Dec. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 34. | | RODRIGUES, V. G. S.; NUNES, C. V. de N. dos S.; OLIVEIRA, V. B. V. de; SOUZA, A. C. R. de; SANTOS, M. R. A. dos; LIMA, M. R. de. Relações ambientais, socioeconômicas e culturais no uso de plantas medicinais na comunidade de Cujubim Grande, Porto Velho - RO. In: REUNIÃO AMAZÔNICA DE AGROECOLOGIA, 1., 2007, Manaus. A agroecologia no contexto amazônico: palestras, relatos de experiência e resumos. Manaus: Embrapa Amazônia Ocidental, 2007. 1 CD ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Rondônia. |
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| 35. | | TORRES, T. R; LUDKE, M. do C. M. M.; RABELLO, C. B. V.; LUDKE, J. V.; SOUZA, E. J. O. de; FARIA, M. A. M. de; COSTA, A. A. G.; LIMA, M. R. de; ANDRADE, E. M. de S. R. de; ASSUNÇÃO FILHO, A. F. Uso de diferentes formas físicas com inclusão de milheto (Pennisetum glaucum) em dietas para frangos de corte In: JORNADA DE ENSINO, PESQUISA E EXTENSÃO DA UFRPE, 9; SEMANA NACIONAL DE CIÊNCIA E TECNOLOGIA, 6; 2009, Recife. Anais.. Recife: UFRPE, 2009. 1 CD-ROM. Projeto/Plano de Ação: 16.00.30004-00| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 35 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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