|
|
Registro Completo |
Biblioteca(s): |
Embrapa Agrossilvipastoril. |
Data corrente: |
18/02/2019 |
Data da última atualização: |
18/02/2019 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
LIMA, L. R.; MOMBACH, M. A.; PEDREIRA, B. C. e; FERREIRA, D. C.; CARVALHO, P. de; AMORIM, K. R. R. de; WEIMER, P. J.; CABRAL, L. da S. |
Afiliação: |
LENI RODRIGUES LIMA, UFMT, CUIABA, MT; MIRCÉIA ANGELE MOMBACH, UFMT, CUIABA, MT; BRUNO CARNEIRO E PEDREIRA, CPAMT; DANIELA CRISTINA FERREIRA, UFMT, CUIABA, MT; PERIVALDO DE CARVALHO, UFMT, CUIABA, MT; KARITHA REGIANE RIBEIRO DE AMORIM, UFMT, CUIABA, MT; PAUL JAMES WEIMER, UNIVERSITY OF WISCONSIN, MADISON, US; LUCIANO DA SILVA CABRAL, UFMT, CUIABA, MT. |
Título: |
Methanogens and rumen protozoa in cattle during transition to high grain diets. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 55.; CONGRESSO BRASILEIRO DE ZOOTECNIA, 28., 2018, Goiânia. Construindo saberes, formando pessoas e transformando a produção animal: anais. Viçosa: Sociedade Brasileira de Zootecnia; Brasília, DF: Associação Brasileira de Zootecnistas. |
Páginas: |
não paginado. |
Idioma: |
Inglês |
Conteúdo: |
The rumen is a very complex ecosystem in which there is a high diversity and concentration of rumen microbes. Animals, microbial and diets effects shape the rumen microbial population what make the rumen microbial manipulation a hard task to be done. There are many aspects about rumen microbial what the scientists and nutritionists would like to change, but the reduction in rumen methanogens and its activities is probably the first one. Considering the probable association between protozoa and rumen methanogens, this study aimed to evaluate their populations in the rumen of beef cattle during transition from a forage to high grains diets by qPCR. Four rumen fitted male Nellore Cattle were used, which were submit to diets from Brachiaria brizantha grass to high grains, from which rumen samples (solid and fluid) were collected when the diets were composed by zero (grazing animals), 50, 60, 70, 80 and 90% of concentrate on dry matter basis, as well as to get pH measurements. The DNA of rumen microbial population was isolated by using beads, phenol and chloroform, which makes possible to get high quantity and quality of DNA isolated. The DNA of rumen microbial population was amplified using groups primers (protozoa and methanogens) by qPCR method, which permits to calculate the genes copies numbers for every group. The rumen pH ranged from 6.51 to 5.73 for forage fed animals and for 90% concentrate fed animals, respectively. We found that protozoa numbers (log10 genes copies) were only reduced for animals fed with 90% concentrate diet (4.00) compared to other diets (forage, 50, 60, 70 and 80%, 5.07 log10 genes copies). The decrease on protozoa numbers may be related to rumen pH, in which protozoa numbers drop 21% only when rumen pH was lower that 5.8 (80% concentrate in the diets). However, the methanogens numbers were higher in forage fed animals (4.40 log10 genes copies) that all grains diets (3.71 log10 genes copies). Thus, there was not a clear association between protozoa and methanogens numbers in the rumen, what has also been observed in other studies done recently using molecular tools. Although we did not have any data about methane emissions in this study, we could speculate the higher concentrate diets (more than 50% concentrate), there would be a lower methane emission, considering the low methanogens numbers in the rumen. MenosThe rumen is a very complex ecosystem in which there is a high diversity and concentration of rumen microbes. Animals, microbial and diets effects shape the rumen microbial population what make the rumen microbial manipulation a hard task to be done. There are many aspects about rumen microbial what the scientists and nutritionists would like to change, but the reduction in rumen methanogens and its activities is probably the first one. Considering the probable association between protozoa and rumen methanogens, this study aimed to evaluate their populations in the rumen of beef cattle during transition from a forage to high grains diets by qPCR. Four rumen fitted male Nellore Cattle were used, which were submit to diets from Brachiaria brizantha grass to high grains, from which rumen samples (solid and fluid) were collected when the diets were composed by zero (grazing animals), 50, 60, 70, 80 and 90% of concentrate on dry matter basis, as well as to get pH measurements. The DNA of rumen microbial population was isolated by using beads, phenol and chloroform, which makes possible to get high quantity and quality of DNA isolated. The DNA of rumen microbial population was amplified using groups primers (protozoa and methanogens) by qPCR method, which permits to calculate the genes copies numbers for every group. The rumen pH ranged from 6.51 to 5.73 for forage fed animals and for 90% concentrate fed animals, respectively. We found that protozoa numbers (log10 genes copies) we... Mostrar Tudo |
Thesaurus Nal: |
Archaea; Methanogens; Rumen microorganisms. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/192971/1/2018-cpamt-bruno-pedreira-methanogens-rumen-protozoa-cattle-transition-grains-diets.pdf
|
Marc: |
LEADER 03328nam a2200241 a 4500 001 2106183 005 2019-02-18 008 2018 bl uuuu u00u1 u #d 100 1 $aLIMA, L. R. 245 $aMethanogens and rumen protozoa in cattle during transition to high grain diets.$h[electronic resource] 260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 55.; CONGRESSO BRASILEIRO DE ZOOTECNIA, 28., 2018, Goiânia. Construindo saberes, formando pessoas e transformando a produção animal: anais. Viçosa: Sociedade Brasileira de Zootecnia; Brasília, DF: Associação Brasileira de Zootecnistas.$c2018 300 $anão paginado. 520 $aThe rumen is a very complex ecosystem in which there is a high diversity and concentration of rumen microbes. Animals, microbial and diets effects shape the rumen microbial population what make the rumen microbial manipulation a hard task to be done. There are many aspects about rumen microbial what the scientists and nutritionists would like to change, but the reduction in rumen methanogens and its activities is probably the first one. Considering the probable association between protozoa and rumen methanogens, this study aimed to evaluate their populations in the rumen of beef cattle during transition from a forage to high grains diets by qPCR. Four rumen fitted male Nellore Cattle were used, which were submit to diets from Brachiaria brizantha grass to high grains, from which rumen samples (solid and fluid) were collected when the diets were composed by zero (grazing animals), 50, 60, 70, 80 and 90% of concentrate on dry matter basis, as well as to get pH measurements. The DNA of rumen microbial population was isolated by using beads, phenol and chloroform, which makes possible to get high quantity and quality of DNA isolated. The DNA of rumen microbial population was amplified using groups primers (protozoa and methanogens) by qPCR method, which permits to calculate the genes copies numbers for every group. The rumen pH ranged from 6.51 to 5.73 for forage fed animals and for 90% concentrate fed animals, respectively. We found that protozoa numbers (log10 genes copies) were only reduced for animals fed with 90% concentrate diet (4.00) compared to other diets (forage, 50, 60, 70 and 80%, 5.07 log10 genes copies). The decrease on protozoa numbers may be related to rumen pH, in which protozoa numbers drop 21% only when rumen pH was lower that 5.8 (80% concentrate in the diets). However, the methanogens numbers were higher in forage fed animals (4.40 log10 genes copies) that all grains diets (3.71 log10 genes copies). Thus, there was not a clear association between protozoa and methanogens numbers in the rumen, what has also been observed in other studies done recently using molecular tools. Although we did not have any data about methane emissions in this study, we could speculate the higher concentrate diets (more than 50% concentrate), there would be a lower methane emission, considering the low methanogens numbers in the rumen. 650 $aArchaea 650 $aMethanogens 650 $aRumen microorganisms 700 1 $aMOMBACH, M. A. 700 1 $aPEDREIRA, B. C. e 700 1 $aFERREIRA, D. C. 700 1 $aCARVALHO, P. de 700 1 $aAMORIM, K. R. R. de 700 1 $aWEIMER, P. J. 700 1 $aCABRAL, L. da S.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Agrossilvipastoril (CPAMT) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
06/09/2023 |
Data da última atualização: |
06/09/2023 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
NEUMAIER, N.; CRUSIOL, L. G. T.; SIBALDELLI, R. N. R.; MARIN, D. R.; SILVA, B. M.; NOGUEIRA, M. A.; CASTRO, C. de; GONCALVES, S. L.; FOLONI, J. S. S.; FARIAS, J. R. B. |
Afiliação: |
NORMAN NEUMAIER, CNPSO; L. G. T. CRUSIOL; RUBSON NATAL RIBEIRO SIBALDELLI, CNPSO; D. R. MARIN, UEL; B. M. SILVA, UNOPAR; MARCO ANTONIO NOGUEIRA, CNPSO; CESAR DE CASTRO, CNPSO; SERGIO LUIZ GONCALVES, CNPSO; JOSE SALVADOR SIMONETTO FOLONI, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO. |
Título: |
Encarquilhamento foliar em soja - dados preliminares para a caracterização fisiológica. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
In: REUNIÃO DE PESQUISA DE SOJA, 38., 2023, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2023. (Embrapa Soja. Eventos técnicos & científicos, 1). Editado por Fernando Augusto Henning, Regina Maria Villas Bôas de Campos Leite. RPS 2023. |
Páginas: |
p. 43-46. |
Idioma: |
Português |
Thesagro: |
Área Foliar; Fisiologia Vegetal; Soja. |
Thesaurus NAL: |
Plant physiology; Soybeans. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156483/1/p-43-ETC-1-RPS-2023-res-exp-2-7.pdf
|
Marc: |
LEADER 01013nam a2200277 a 4500 001 2156483 005 2023-09-06 008 2023 bl uuuu u00u1 u #d 100 1 $aNEUMAIER, N. 245 $aEncarquilhamento foliar em soja - dados preliminares para a caracterização fisiológica.$h[electronic resource] 260 $aIn: REUNIÃO DE PESQUISA DE SOJA, 38., 2023, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2023. (Embrapa Soja. Eventos técnicos & científicos, 1). Editado por Fernando Augusto Henning, Regina Maria Villas Bôas de Campos Leite. RPS 2023.$c2023 300 $ap. 43-46. 650 $aPlant physiology 650 $aSoybeans 650 $aÁrea Foliar 650 $aFisiologia Vegetal 650 $aSoja 700 1 $aCRUSIOL, L. G. T. 700 1 $aSIBALDELLI, R. N. R. 700 1 $aMARIN, D. R. 700 1 $aSILVA, B. M. 700 1 $aNOGUEIRA, M. A. 700 1 $aCASTRO, C. de 700 1 $aGONCALVES, S. L. 700 1 $aFOLONI, J. S. S. 700 1 $aFARIAS, J. R. B.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Soja (CNPSO) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|