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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
10/02/2020 |
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Data da última atualização: |
28/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LIMA, L. G. de; SOUZA, N. O. B. de; RIOS, R. R.; MELO, B. A. de; SANTOS, L. T. A. dos; SILVA, K. de M.; MURPHY, T. W.; FRAGA, A. B. |
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Afiliação: |
LUCIANO GOMES DE LIMA, Northeastern Network in Biotechnology (RENORBIO), Brazil; Federal University of Alagoas (UFAL) - Maceió, AL, Brazil; NAMÍBIA OLIVEIRA BALBINO DE SOUZA, Federal University of Alagoas (UFAL) - Rio Largo, AL, Brazil; RAISA RODRIGUES RIOS, Northeastern Network in Biotechnology (RENORBIO), Brazil; BRENO ARAÚJO DE MELO, Northeastern Network in Biotechnology (RENORBIO), Brazil; LAYS THAYSE ALVES DOS SANTOS, Federal University of Alagoas (UFAL) - Rio Largo, AL, Brazil; KLEIBE DE MORAES SILVA, CNPC; THOMAS WAYNE MURPHY, dDepartment of Animal and Range Sciences, Montana State University - Bozeman, MT, USA; ANGELINA BOSSI FRAGA, Federal University of Alagoas (UFAL) - Rio Largo, AL, Brazil. |
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Título: |
Advances in molecular genetic techniques applied to selection for litter size in goats (Capra hircus): a review. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Applied Animal Research, v. 48, n. 1, p. 38-44, 2020. |
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DOI: |
10.1080/09712119.2020.1717497 |
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Idioma: |
Inglês |
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Conteúdo: |
Litter size, or prolificacy, in goats is defined as the number of kids born per doe kidding. Improving litter size through selection not only directly enhances producer profitability as more progeny can be marketed but can also increase genetic gains in other traits due to greater selection intensity. However, most traits associated with reproduction have low heritability, and genetic improvement will be slow if the selection is based on one or a few phenotypic records. In the absence of a genetic evaluation programme with extensive pedigrees and performance recording, phenotypic selection for litter size is not promising. Advances in molecular genetic techniques may serve as an alternative to increase genetic progress in prolificacy. Several techniques have been developed to elucidate the mechanisms involved in phenotypic expression at the DNA level. Although recent research has identified genomic regions associated with several production traits in goats, litter size has not been extensively researched. Nevertheless, recent advancements in molecular genetic have created new opportunities for the improvement of litter size in goats. The development of next generation molecular tools to identify genomic genetic variants has made it possible to apply whole-genome scanning techniques, genomewide association studies, and genomic selection to improve goat prolificacy. |
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Palavras-Chave: |
Molecular markers; Prolificacy; Single Nucleotide Polymorphisms. |
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Thesagro: |
Caprino; Engenharia Genética; Genética Animal; Marcador Genético; Polimorfismo Genético. |
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Thesaurus Nal: |
Animal genetics; Genetic markers; Genome-wide association study; Goats; Polymorphism. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02522naa a2200373 a 4500
001 2120055
005 2020-04-28
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1080/09712119.2020.1717497$2DOI
100 1 $aLIMA, L. G. de
245 $aAdvances in molecular genetic techniques applied to selection for litter size in goats (Capra hircus)$ba review.$h[electronic resource]
260 $c2020
520 $aLitter size, or prolificacy, in goats is defined as the number of kids born per doe kidding. Improving litter size through selection not only directly enhances producer profitability as more progeny can be marketed but can also increase genetic gains in other traits due to greater selection intensity. However, most traits associated with reproduction have low heritability, and genetic improvement will be slow if the selection is based on one or a few phenotypic records. In the absence of a genetic evaluation programme with extensive pedigrees and performance recording, phenotypic selection for litter size is not promising. Advances in molecular genetic techniques may serve as an alternative to increase genetic progress in prolificacy. Several techniques have been developed to elucidate the mechanisms involved in phenotypic expression at the DNA level. Although recent research has identified genomic regions associated with several production traits in goats, litter size has not been extensively researched. Nevertheless, recent advancements in molecular genetic have created new opportunities for the improvement of litter size in goats. The development of next generation molecular tools to identify genomic genetic variants has made it possible to apply whole-genome scanning techniques, genomewide association studies, and genomic selection to improve goat prolificacy.
650 $aAnimal genetics
650 $aGenetic markers
650 $aGenome-wide association study
650 $aGoats
650 $aPolymorphism
650 $aCaprino
650 $aEngenharia Genética
650 $aGenética Animal
650 $aMarcador Genético
650 $aPolimorfismo Genético
653 $aMolecular markers
653 $aProlificacy
653 $aSingle Nucleotide Polymorphisms
700 1 $aSOUZA, N. O. B. de
700 1 $aRIOS, R. R.
700 1 $aMELO, B. A. de
700 1 $aSANTOS, L. T. A. dos
700 1 $aSILVA, K. de M.
700 1 $aMURPHY, T. W.
700 1 $aFRAGA, A. B.
773 $tJournal of Applied Animal Research$gv. 48, n. 1, p. 38-44, 2020.
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Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
07/12/2007 |
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Data da última atualização: |
11/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
FERNANDEZ, J. H.; MELLO, M. O; GALGARO, L.; TANAKA, A. S.; SILVA-FILHO, M. C.; NESHICH, G. |
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Afiliação: |
LNCC, Petrópolis, RJ; Esalq/USP; Esalq/USP; Unifesp; Esalq/USP; GORAN NESHICH, CNPTIA. |
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Título: |
Proteinase inhibition using small Bowman-Birk-type structures. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 6, n. 4, p. 846-858, 2007. |
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Idioma: |
Inglês |
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Conteúdo: |
Absttract . Bowman-Birk inhibitors (BBIs) are cysteine-rich and highly cross-linked small proteins that function as specific pseudosubstrates for digestive proteinases. They typically display a "double-headed" structure containing an independent proteinase-binding loop that can bind and inhibit trypsin, chymotrypsin and elastase. In the present study, we used computational biology to study the structural characteristics and dynamics of the inhibition mechanism of the small BBI loop expressing a 35-amino acid polypeptide (ChyTB2 inhibitor) which has coding region for the mutated chymotrypsin-inhibitory site of the soybean BBI. We found that in the BBI-trypsin inhibition complex, the most important interactions are salt bridges and hydrogen bonds, whereas in the BBI-chymotrypsin inhibition complex, the most important interactions are hydrophobic. At the same time, ChyTB2 mutant structure maintained the individual functional domain structure and excellent binding/inhibiting capacities for trypsin and chymotrypsin at the same time. These results were confirmed by enzyme-linked immunosorbend assay experiments. The results showed that modeling combined with molecular dynamics is an efficient method to describe, predict and then obtain new proteinase inhibitors. For such study, however, it is necessary to start from the sequence and structure of the mutant interacting relatively strongly with both trypsin and chymotrypsin for designing the small BBI-type inhibitor against proteinases. MenosAbsttract . Bowman-Birk inhibitors (BBIs) are cysteine-rich and highly cross-linked small proteins that function as specific pseudosubstrates for digestive proteinases. They typically display a "double-headed" structure containing an independent proteinase-binding loop that can bind and inhibit trypsin, chymotrypsin and elastase. In the present study, we used computational biology to study the structural characteristics and dynamics of the inhibition mechanism of the small BBI loop expressing a 35-amino acid polypeptide (ChyTB2 inhibitor) which has coding region for the mutated chymotrypsin-inhibitory site of the soybean BBI. We found that in the BBI-trypsin inhibition complex, the most important interactions are salt bridges and hydrogen bonds, whereas in the BBI-chymotrypsin inhibition complex, the most important interactions are hydrophobic. At the same time, ChyTB2 mutant structure maintained the individual functional domain structure and excellent binding/inhibiting capacities for trypsin and chymotrypsin at the same time. These results were confirmed by enzyme-linked immunosorbend assay experiments. The results showed that modeling combined with molecular dynamics is an efficient method to describe, predict and then obtain new proteinase inhibitors. For such study, however, it is necessary to start from the sequence and structure of the mutant interacting relatively strongly with both trypsin and chymotrypsin for designing the small BBI-type inhibitor against proteinas... Mostrar Tudo |
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Palavras-Chave: |
Bioinformática; Enzyme specificity; Inibidor Bowman-Birk; Modelagem molecular; Molecular modeling. |
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Thesagro: |
Proteína. |
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Thesaurus NAL: |
Bioinformatics; Enzyme-linked immunosorbent assay; Models. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/159697/1/AP-Proteinase-GMR-2007.pdf
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Marc: |
LEADER 02330naa a2200289 a 4500
001 1000764
005 2017-05-11
008 2007 bl uuuu u00u1 u #d
100 1 $aFERNANDEZ, J. H.
245 $aProteinase inhibition using small Bowman-Birk-type structures.$h[electronic resource]
260 $c2007
520 $aAbsttract . Bowman-Birk inhibitors (BBIs) are cysteine-rich and highly cross-linked small proteins that function as specific pseudosubstrates for digestive proteinases. They typically display a "double-headed" structure containing an independent proteinase-binding loop that can bind and inhibit trypsin, chymotrypsin and elastase. In the present study, we used computational biology to study the structural characteristics and dynamics of the inhibition mechanism of the small BBI loop expressing a 35-amino acid polypeptide (ChyTB2 inhibitor) which has coding region for the mutated chymotrypsin-inhibitory site of the soybean BBI. We found that in the BBI-trypsin inhibition complex, the most important interactions are salt bridges and hydrogen bonds, whereas in the BBI-chymotrypsin inhibition complex, the most important interactions are hydrophobic. At the same time, ChyTB2 mutant structure maintained the individual functional domain structure and excellent binding/inhibiting capacities for trypsin and chymotrypsin at the same time. These results were confirmed by enzyme-linked immunosorbend assay experiments. The results showed that modeling combined with molecular dynamics is an efficient method to describe, predict and then obtain new proteinase inhibitors. For such study, however, it is necessary to start from the sequence and structure of the mutant interacting relatively strongly with both trypsin and chymotrypsin for designing the small BBI-type inhibitor against proteinases.
650 $aBioinformatics
650 $aEnzyme-linked immunosorbent assay
650 $aModels
650 $aProteína
653 $aBioinformática
653 $aEnzyme specificity
653 $aInibidor Bowman-Birk
653 $aModelagem molecular
653 $aMolecular modeling
700 1 $aMELLO, M. O
700 1 $aGALGARO, L.
700 1 $aTANAKA, A. S.
700 1 $aSILVA-FILHO, M. C.
700 1 $aNESHICH, G.
773 $tGenetics and Molecular Research$gv. 6, n. 4, p. 846-858, 2007.
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Embrapa Agricultura Digital (CNPTIA) |
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