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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
18/10/2001 |
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Data da última atualização: |
18/10/2001 |
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Autoria: |
LIMA, J. A. A. |
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Título: |
Blackeye cowpea mosaic virus: purification, partial characterization, serology, and immunochemical and cytological techniques for detection of virus-infected legume seeds. |
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Ano de publicação: |
1978 |
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Fonte/Imprenta: |
[S.l]: University of Florida, 1978. |
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Páginas: |
153p. |
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Idioma: |
Inglês |
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Notas: |
PhD. Dissertation. |
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Conteúdo: |
Blackeye cowpea mosaic virus (BICMV) was increased in cowpea, Vigna unguiculata (L.) Walp., 'knuckle purple hull', and infected leaves were used for virus and cytoplasmic inclusion purification. Either n-butanol or a combination of chloroform and carbon tetrachloride was used in the clacification process. Polyacrylamide gel electrophoresis of sodium dodecyl sulfate(SDS) dissociated inclusions and virus revealed that the inclusions were made of a single protein estimated to have e molecular weight (MW) around 70.000 daltons whereas freshly purified BICMV consisted of a main protein component with a MW of 34,000 da itons and two smaller proteins with WMs of 29,000 and 27,000 daltons, Purified BICMV had a 260/280 nm absorption ration of 1.2 and a modal length of 753 nm. Freshly purified BICMV preparations showed a single sedimenting peak with s20 =157-159 s. The purified BICMV cytoplasmic inclusions had absorption spectra characteristic for proteins. Electron microscopy of purified inclusions revealed the presence of tubes showing striations with periodicities of approximately 5nm. Antisera reactive in SDS-immunodiffusion were obtainedagainst untreated virions, pyrrolidine degrasded ceat protein, and untreated BICMV cytoplasmic incluions. Reciprocal double immunodiffusion tests with SDS-treated antigens showed that BICMV is serologically unrelated to seven potyviruses and serologically related to, but distinct from: bean common mosaic virus (BCMV), bean yellow mosaic virus (BYMC), cowpea aphid-borne mosaic virus CAMV), dasheen mosaic (DMV), lettuce mosaic virus (LMV), potato virus y (PVY), soybean mosaic virus (SoyMV), tobacco etch virus (TEV), and watermelon masaic virus-2 (WMV-2). The intregal cross-absorption technique was also used to demonstrate distinction between closely related potyviruses. Ager medium impregnated with a mixture of antisera used for serodiagnosis of BICMV and cowpea mosaic virus in cowpea. Light and electron microscopy of cytoplasmic inclusions induced by BICMV, siratro (Macroptilium atropurpureum (D.C.) Urb.) strain of BCMV (BCMV-S) and CAMV revealed that they are similar to those induced by the potyviruses from Edwardson's subdivision-l. The different reactions induced by BICMV, and CAMV in some cowpea varieties in-dicated that they can also be used as used as differential hosts for these three potyviruses. Sources of resistance for BICMV were found among the cowpea varieties tested. Based on its physical, biological, cytological, and immunochemical properties, BICMV can be differentiated fron any other virus that infects cowpea. Cytoplasmic inclusions induced by BICMV in cowpea and by SoyMV in soybean were detected by serology, light microscopy, and alactron microscopy in hypocotyls of 4-5-day-old seedlings grown from virus-infected seeds. Immunodiffusion tests and serologically specific electrom micro-scopy were used to detect BICMV in hypocotyls of 4-5-day-old cowpea seedlings grown from BICMV-infected seeds. Discs of individual hypocotyls were embedded into the agar medium 4-5 mm away from the antiserum wells. Virus-specific precipitin lines formed between virus-infected hypocotyl discs and antiserum wells, whereas no reactinos were observed with healthy hypocotyls. Precipitin lines were also observed with extracts of mixtures from infected (1 g) and healthy (up to 29 g) tissues. These immunochemical techniques were also used for detecting BCMC in hypocotyls of infdcted 4-5-day-old phaseolus vulgaris L. seedlings and for detecting SoyMV in infected Glycine max (L) Merr. seedlings. Single radial immunodiffusion tests with extracts or discs of cowpea hypocotyls were also useful for detecting BICMV in germinated seeds. The reliability and simplicity of the immunodiffusion tests make them suitable for use in routine seed health testing program in any laboratory. MenosBlackeye cowpea mosaic virus (BICMV) was increased in cowpea, Vigna unguiculata (L.) Walp., 'knuckle purple hull', and infected leaves were used for virus and cytoplasmic inclusion purification. Either n-butanol or a combination of chloroform and carbon tetrachloride was used in the clacification process. Polyacrylamide gel electrophoresis of sodium dodecyl sulfate(SDS) dissociated inclusions and virus revealed that the inclusions were made of a single protein estimated to have e molecular weight (MW) around 70.000 daltons whereas freshly purified BICMV consisted of a main protein component with a MW of 34,000 da itons and two smaller proteins with WMs of 29,000 and 27,000 daltons, Purified BICMV had a 260/280 nm absorption ration of 1.2 and a modal length of 753 nm. Freshly purified BICMV preparations showed a single sedimenting peak with s20 =157-159 s. The purified BICMV cytoplasmic inclusions had absorption spectra characteristic for proteins. Electron microscopy of purified inclusions revealed the presence of tubes showing striations with periodicities of approximately 5nm. Antisera reactive in SDS-immunodiffusion were obtainedagainst untreated virions, pyrrolidine degrasded ceat protein, and untreated BICMV cytoplasmic incluions. Reciprocal double immunodiffusion tests with SDS-treated antigens showed that BICMV is serologically unrelated to seven potyviruses and serologically related to, but distinct from: bean common mosaic virus (BCMV), bean yellow mosaic virus (BYM... Mostrar Tudo |
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Palavras-Chave: |
Cowpea mosaic comovirus; Plant diseases; Seed. |
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Thesagro: |
Doença; Feijão de Corda; Semente; Vigna Sinensis; Vigna Unguiculata; Vírus. |
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Thesaurus Nal: |
cowpeas; viruses. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 04631nam a2200265 a 4500
001 1560262
005 2001-10-18
008 1978 bl uuuu m 00u1 u #d
100 1 $aLIMA, J. A. A.
245 $aBlackeye cowpea mosaic virus$bpurification, partial characterization, serology, and immunochemical and cytological techniques for detection of virus-infected legume seeds.
260 $a[S.l]: University of Florida$c1978
300 $a153p.
500 $aPhD. Dissertation.
520 $aBlackeye cowpea mosaic virus (BICMV) was increased in cowpea, Vigna unguiculata (L.) Walp., 'knuckle purple hull', and infected leaves were used for virus and cytoplasmic inclusion purification. Either n-butanol or a combination of chloroform and carbon tetrachloride was used in the clacification process. Polyacrylamide gel electrophoresis of sodium dodecyl sulfate(SDS) dissociated inclusions and virus revealed that the inclusions were made of a single protein estimated to have e molecular weight (MW) around 70.000 daltons whereas freshly purified BICMV consisted of a main protein component with a MW of 34,000 da itons and two smaller proteins with WMs of 29,000 and 27,000 daltons, Purified BICMV had a 260/280 nm absorption ration of 1.2 and a modal length of 753 nm. Freshly purified BICMV preparations showed a single sedimenting peak with s20 =157-159 s. The purified BICMV cytoplasmic inclusions had absorption spectra characteristic for proteins. Electron microscopy of purified inclusions revealed the presence of tubes showing striations with periodicities of approximately 5nm. Antisera reactive in SDS-immunodiffusion were obtainedagainst untreated virions, pyrrolidine degrasded ceat protein, and untreated BICMV cytoplasmic incluions. Reciprocal double immunodiffusion tests with SDS-treated antigens showed that BICMV is serologically unrelated to seven potyviruses and serologically related to, but distinct from: bean common mosaic virus (BCMV), bean yellow mosaic virus (BYMC), cowpea aphid-borne mosaic virus CAMV), dasheen mosaic (DMV), lettuce mosaic virus (LMV), potato virus y (PVY), soybean mosaic virus (SoyMV), tobacco etch virus (TEV), and watermelon masaic virus-2 (WMV-2). The intregal cross-absorption technique was also used to demonstrate distinction between closely related potyviruses. Ager medium impregnated with a mixture of antisera used for serodiagnosis of BICMV and cowpea mosaic virus in cowpea. Light and electron microscopy of cytoplasmic inclusions induced by BICMV, siratro (Macroptilium atropurpureum (D.C.) Urb.) strain of BCMV (BCMV-S) and CAMV revealed that they are similar to those induced by the potyviruses from Edwardson's subdivision-l. The different reactions induced by BICMV, and CAMV in some cowpea varieties in-dicated that they can also be used as used as differential hosts for these three potyviruses. Sources of resistance for BICMV were found among the cowpea varieties tested. Based on its physical, biological, cytological, and immunochemical properties, BICMV can be differentiated fron any other virus that infects cowpea. Cytoplasmic inclusions induced by BICMV in cowpea and by SoyMV in soybean were detected by serology, light microscopy, and alactron microscopy in hypocotyls of 4-5-day-old seedlings grown from virus-infected seeds. Immunodiffusion tests and serologically specific electrom micro-scopy were used to detect BICMV in hypocotyls of 4-5-day-old cowpea seedlings grown from BICMV-infected seeds. Discs of individual hypocotyls were embedded into the agar medium 4-5 mm away from the antiserum wells. Virus-specific precipitin lines formed between virus-infected hypocotyl discs and antiserum wells, whereas no reactinos were observed with healthy hypocotyls. Precipitin lines were also observed with extracts of mixtures from infected (1 g) and healthy (up to 29 g) tissues. These immunochemical techniques were also used for detecting BCMC in hypocotyls of infdcted 4-5-day-old phaseolus vulgaris L. seedlings and for detecting SoyMV in infected Glycine max (L) Merr. seedlings. Single radial immunodiffusion tests with extracts or discs of cowpea hypocotyls were also useful for detecting BICMV in germinated seeds. The reliability and simplicity of the immunodiffusion tests make them suitable for use in routine seed health testing program in any laboratory.
650 $acowpeas
650 $aviruses
650 $aDoença
650 $aFeijão de Corda
650 $aSemente
650 $aVigna Sinensis
650 $aVigna Unguiculata
650 $aVírus
653 $aCowpea mosaic comovirus
653 $aPlant diseases
653 $aSeed
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Registro original: |
Embrapa Cerrados (CPAC) |
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| Registros recuperados : 536 | |
| 441. |  | SILVEIRA, D. T.; BORGES, C. S.; CUCHIARA, C. C.; MARQUES, F. P.; SILVA, S. D. dos A. e.; BOBROWSKI, V. L. Otimização da análise de cromossomos mitóticos de mamona (Ricinus communis L.) In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 1.; REUNIÃO TÉCNICA ANUAL DE AGROENERGIA-RS, 1., 2007, Pelotas. Anais... Pelotas: Embrapa Clima Temperado, 2007. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Clima Temperado. |
|   |
| 442. | | CASAGRANDE JÚNIOR, J. G.; SILVA, S. D. dos A. e.; EICHOLZ, E. D.; MIGOM, L.; RADÜZ, L. L.; STECKLING, C.; MOREIRA, L. L. Reação de genótipos comerciais de mamona a Meloidogyne incognita. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 2.; REUNIÃO TÉCNICA ANUAL DE AGROENERGIA, 2., 2008, Porto Alegre. Anais... Pelotas: Embrapa Clima Temperado, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 444. | | LIMA, C. L. R. de L.; PILLON, C. N.; LIMA, A. C. R. de; SILVA, S. D. dos A. e.; SCHLEE, B. N. Qualidade física e química de um argissolo sob culturas promissoras a produção de bioenergia. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 1.; REUNIÃO TÉCNICA ANUAL DE AGROENERGIA-RS, 1., 2007, Pelotas. Anais... Pelotas: Embrapa Clima Temperado, 2007. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 445. | | KUNDE, R. J.; LEAL, O. dos A.; SANTOS, D. C. dos; VIGNOLO, G. K.; LIMA, C. L. R. de; SILVA, S. D. dos A. e; PILLON, C. N. Propriedades químicas de um argissolo sob plantio direto de culturas promissoras a produção de biocombustível. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA ANUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA DA MANDIOCA, 10.; REUNIÃO TÉCNICA DA BATATA-DOCE, 2., Pelotas, 2010. Anais... Pelotas: Embrapa Clima Temperado, 2010. Organizado por Sérgio Delmar dos Anjos e Silva, Ivan Rodrigues de Almeida e Ana Cláudia Barneche de Oliveira. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 446. | | FARIAS, M. de O.; PILLON, C. N.; LIMA, C. L. R. de; SANTOS, D. C. dos S.; SILVA, S. D. dos A. e.; DUPONT, P. B. Propriedades químicas em macro e microagregado de um argissolo sob plantio direto de milho e mamona. In: REUNIÃO BRASILEIRA DE MANEJO E CONSERVAÇÃO DO SOLO E DA ÁGUA, 17., 2008, Rio de Janeiro. Manejo e conservação do solo e da água no contexto das mudanças ambientais. Rio de Janeiro: SBCS: Embrapa Solos: Embrapa Agrobiologia, 2008. 1 CD ROM. (Embrapa Solos. Documentos, 101).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 448. | | DONINI, L. P.; MAYER, K. C. de A.; ZACARIAS, F. M.; SILVA, N. D. G. da; VITÓRIA, J. M.; SILVA, S. D. dos A. e; DUTRA, L. F. Produção de mudas de cana-de-açúcar (saccharum officinarum l.) Micropropagadas. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 4.; REUNIÃO TÉCNICA DE AGROENERGIA, 4., 2012, AMRIGS: Porto Alegre. Anais... Pelotas: Embrapa Clima Temperado, 2012. Organizado por Sérgio Delmar dos Anjos e Silva Ivan Rodrigues de Almeida.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
|  |
| 449. | | OLIVEIRA, R. J. P. de; SILVA, S. D. dos A. e; CAMPOS, A. D. S. de; RIBEIRO, E. F.; HÄRTER, A.; FERREIRA NETO, D. T. Potencial produtivo dos genótipos elite de pinhão-manso na safra de 2012/13 no município de Pelotas/RS. In: SIMPÓSIO ESTADUAL DE AGROENERGIA; REUNIÃO TÉCNICA DE AGROENERGIA - RS, 5.; ENCONTRO DE ENERGIAS RENOVÁVEIS NA AGRICULTURA FAMILIAR, 2., Pelotas, 2014. Anais... Pelotas: Embrapa Clima Temperado, 2014. 1 pen card.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 450. | | THEISEN, G.; FIPKE, M. V.; BONOW, J. F. L.; REIS, A.; XAVIER, F. da M.; NEMITZ, C.; SILVA, S. D. dos A. e. Produtividade de cultivares de cana-de-acucar (saccharum officinarum l.) cultivadas em terras baixas nas safras 2010/2011 e 2011/2012. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 4.; REUNIÃO TÉCNICA DE AGROENERGIA, 4., 2012, AMRIGS: Porto Alegre. Anais... Pelotas: Embrapa Clima Temperado, 2012. Organizado por Sérgio Delmar dos Anjos e Silva Ivan Rodrigues de Almeida.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 451. | | VIGNOLO, G. K.; ARAÚJO, V. F.; CARVALHO, G. L.; COCCO, C.; SILVEIRA, C. A. P.; ANTUNES, L. E. C.; SILVA, S. D. dos A. e. Produção de melão sob diferentes doses de torta de mamona, fosforita alvorada e cloreto de potássio em pós cultivo de morangueiro. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA ANUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA DA MANDIOCA, 10.; REUNIÃO TÉCNICA DA BATATA-DOCE, 2., Pelotas, 2010. Anais... Pelotas: Embrapa Clima Temperado, 2010. Organizado por Sérgio Delmar dos Anjos e Silva, Ivan Rodrigues de Almeida e Ana Cláudia Barneche de Oliveira. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 452. | | ÁVILA, D. T. de; ÁVILA, T. T. de; SILVA, S. D. dos A. e; LEMÕES, L. da S.; AIRES, R. F. Propagação assexuada de tungue em Pelotas-RS. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 4.; REUNIÃO TÉCNICA DE AGROENERGIA, 4., 2012, AMRIGS: Porto Alegre. Anais... Pelotas: Embrapa Clima Temperado, 2012. Organizado por Sérgio Delmar dos Anjos e Silva Ivan Rodrigues de Almeida.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 453. | | MORAIS, K. P.; MEDEIROS, S. L. P.; SILVA, S. D. dos A. e; BIONDO, J. C.; BOELTER, J. H.; DIAS, F. S. Produtividade e teor de fibra de genótipos de cana-de-açucar em jaguari-RS. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 4.; REUNIÃO TÉCNICA DE AGROENERGIA, 4., 2012, AMRIGS: Porto Alegre. Anais... Pelotas: Embrapa Clima Temperado, 2012. Organizado por Sérgio Delmar dos Anjos e Silva Ivan Rodrigues de Almeida.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 454. | | CASAGRANDE JÚNIOR, J. G.; ÁVILA, D. T. de; SILVA, S. D. dos A. e.; ÁVILA, T. T. de; AIRES, R. F. Produtividade de Tungue em plantios comerciais na Serra Gaúcha. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 2.; REUNIÃO TÉCNICA ANUAL DE AGROENERGIA, 2., 2008, Porto Alegre. Anais... Pelotas: Embrapa Clima Temperado, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 455. | | ANTUNES, W. R.; SCHÖFFEL, E. R.; SILVA, S. D. dos A. e; HÄRTER, A.; CAMPOS, A. D. S. de; MONTERO, C. R. S. Productive performance of early sugarcane genotypes in rio grande do sul state, Brazil. Revista Scientia Agraria, 18, n. 2, p. 136-142, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 456. | | ÁVILA, D. T.; SILVA, S. D. dos A. e; AIRES, R. F.; ÁVILA, T. T. de; EICHOLZ, E. D.; VERISSIMO, M. A. A. Potencialidade da cultura do Tungue (Aleurites fordii) no Rio Grande do Sul. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA ANUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA DA MANDIOCA, 10.; REUNIÃO TÉCNICA DA BATATA-DOCE, 2., Pelotas, 2010. Anais... Pelotas: Embrapa Clima Temperado, 2010. Organizado por Sérgio Delmar dos Anjos e Silva, Ivan Rodrigues de Almeida e Ana Cláudia Barneche de Oliveira. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 457. | | BORGES FILHO, R. da C.; STURZA, V. S.; BERNARDI, D.; CUNHA, U. S. da; PINTO, A. S.; SILVA, S. D. dos A. e; NAVA, D. E. Population dynamics of pests and natural enemies on sugar cane grown in a subtropical region of Brazil. Florida Entomologist, v. 102, n. 3, p. 526-530, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 458. | | PARAGINSKI, R. T.; FARIAS, S. P.; SILVA, C. F. L. e.; SHIRMER, M. A.; MESSIAS, R. da S.; SILVA, S. D. dos A. e. Rendimento industrial e teor de fibras de quatro cultivares de arroz (Oryza sativa l.) visando a produção de biocombustíveis. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 4.; REUNIÃO TÉCNICA DE AGROENERGIA, 4., 2012, AMRIGS: Porto Alegre. Anais... Pelotas: Embrapa Clima Temperado, 2012. Organizado por Sérgio Delmar dos Anjos e Silva Ivan Rodrigues de Almeida.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 459. | | LEMÕES, J. L.; KNEIB, R. B.; MOREIRA, L. L.; SILVA, S. D. dos A. e.; ANTHONISEN, D. G.; FONSECA, C. P. Seleção de Primers para estudo de variabilidade genética de genótipos de tungue utilizando marcadores RAPD. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 1.; REUNIÃO TÉCNICA ANUAL DE AGROENERGIA-RS, 1., 2007, Pelotas. Anais... Pelotas: Embrapa Clima Temperado, 2007. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 460. | | VERISSIMO, M. A. A.; AZEREDO, A. A. C. de; SILVA, S. D. dos A. e; BRASILEIRO, B. P.; BESPALHOK FILHO, J. C.; DAROS, E.; OLIVEIRA, R. A. de. Selection of sugarcane families and clones under cold stress. Ciência Rural, Santa Maria, v. 50, n. 1, e20181043, 2020. 12 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 536 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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