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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
28/09/2018 |
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Data da última atualização: |
28/09/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GRESSLER, V.; FEDDERN, V.; PEIXOTO, J. de O.; LEDUR, M. C.; DALLA COSTA, O. A.; LIMA, G. J. M. M. de. |
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Afiliação: |
VANESSA GRESSLER, CNPSA; VIVIAN FEDDERN, CNPSA; JANE DE OLIVEIRA PEIXOTO, CNPSA; MONICA CORREA LEDUR, CNPSA; OSMAR ANTONIO DALLA COSTA, CNPSA; GUSTAVO JULIO MELLO M DE LIMA, CNPSA. |
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Título: |
Application of enzyme digestion and deconjugation followed by quick, easy, cheap, effective, rugged, safe extraction and liquid chromatography?tandem mass spectrometry methodology to determine ractopamine residue in pork. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Journal of Food Protection, v. 81, n.8, p. 1258-1263, 2018. |
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DOI: |
10.4315/0362-028X.JFP-17-526 |
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Idioma: |
Inglês |
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Conteúdo: |
A new methodology is proposed for ractopamine residue analysis in pork. It consists of enzyme-mediated digestion and deconjugation steps; modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction; and liquid chromatography?tandem mass spectrometry (LC-MS/MS). In brief, the samples were digested with protease and then deconjugated with b-glucuronidase enzyme; they were then subjected to extraction and cleanup by QuEChERS and underwent sequential analysis by LC-MS/MS. The method performance was evaluated in accordance to the validation guidelines regulated by the Brazilian Ministry of Agriculture and Food Supply. The limit of detection was 0.15 lg/kg and limit of quantification was 0.5 lg/kg. When the method was applied to real samples, ractopamine residue was found in concentrations (up to 7.86 lg/kg) below international recommendation limits up to 10 lg/kg. The method is sensitive, accurate, quick, simple, and suitable for routine analysis; therefore, it is a monitoring tool that may be adopted by laboratories to achieve compliance levels. |
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Palavras-Chave: |
B-agonista; Carne suína; Method validation; Método de validação; Músculo suíno; Ractopamina; Swine muscle. |
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Thesagro: |
Carcaça; Cromatografia; Digestão; Espectrometria; Hidrolise; Nutrição Animal; Suíno. |
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Thesaurus Nal: |
Animal nutrition; Digestion; Hydrolysis; Liquid chromatography; Mass spectrometry; Methodology; Ractopamine; Swine. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02464naa a2200457 a 4500
001 2096560
005 2018-09-28
008 2018 bl uuuu u00u1 u #d
024 7 $a10.4315/0362-028X.JFP-17-526$2DOI
100 1 $aGRESSLER, V.
245 $aApplication of enzyme digestion and deconjugation followed by quick, easy, cheap, effective, rugged, safe extraction and liquid chromatography?tandem mass spectrometry methodology to determine ractopamine residue in pork.$h[electronic resource]
260 $c2018
520 $aA new methodology is proposed for ractopamine residue analysis in pork. It consists of enzyme-mediated digestion and deconjugation steps; modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction; and liquid chromatography?tandem mass spectrometry (LC-MS/MS). In brief, the samples were digested with protease and then deconjugated with b-glucuronidase enzyme; they were then subjected to extraction and cleanup by QuEChERS and underwent sequential analysis by LC-MS/MS. The method performance was evaluated in accordance to the validation guidelines regulated by the Brazilian Ministry of Agriculture and Food Supply. The limit of detection was 0.15 lg/kg and limit of quantification was 0.5 lg/kg. When the method was applied to real samples, ractopamine residue was found in concentrations (up to 7.86 lg/kg) below international recommendation limits up to 10 lg/kg. The method is sensitive, accurate, quick, simple, and suitable for routine analysis; therefore, it is a monitoring tool that may be adopted by laboratories to achieve compliance levels.
650 $aAnimal nutrition
650 $aDigestion
650 $aHydrolysis
650 $aLiquid chromatography
650 $aMass spectrometry
650 $aMethodology
650 $aRactopamine
650 $aSwine
650 $aCarcaça
650 $aCromatografia
650 $aDigestão
650 $aEspectrometria
650 $aHidrolise
650 $aNutrição Animal
650 $aSuíno
653 $aB-agonista
653 $aCarne suína
653 $aMethod validation
653 $aMétodo de validação
653 $aMúsculo suíno
653 $aRactopamina
653 $aSwine muscle
700 1 $aFEDDERN, V.
700 1 $aPEIXOTO, J. de O.
700 1 $aLEDUR, M. C.
700 1 $aDALLA COSTA, O. A.
700 1 $aLIMA, G. J. M. M. de
773 $tJournal of Food Protection$gv. 81, n.8, p. 1258-1263, 2018.
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Embrapa Suínos e Aves (CNPSA) |
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| 2. |  | SILVA, J. C. F. D. DA.; SANTOS, S. A.; SALES, R. L.; CRUZ, L. H. DA.; GARCIA, J. B.; RAVAGLIA, E. Comportamento ingestivo de diferentes categorias de bovinos de corte em pastagens nativas e exóticas durante seca no Pantanal. SIMPÓSIO SOBRE RECURSOS NATURAIS E SOCIOECONÔMICOS DO PANTANAL, 5., 2010, Corumbá, MS. Anais... Corumbá: Embrapa Pantanal: UFMS; Campinas: ICS do Brasil, 2010. 1 CD-ROM SIMPAN 2010. Não Paginado| Tipo: Artigo em Anais de Congresso |
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