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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
28/09/2018 |
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Data da última atualização: |
28/09/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GRESSLER, V.; FEDDERN, V.; PEIXOTO, J. de O.; LEDUR, M. C.; DALLA COSTA, O. A.; LIMA, G. J. M. M. de. |
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Afiliação: |
VANESSA GRESSLER, CNPSA; VIVIAN FEDDERN, CNPSA; JANE DE OLIVEIRA PEIXOTO, CNPSA; MONICA CORREA LEDUR, CNPSA; OSMAR ANTONIO DALLA COSTA, CNPSA; GUSTAVO JULIO MELLO M DE LIMA, CNPSA. |
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Título: |
Application of enzyme digestion and deconjugation followed by quick, easy, cheap, effective, rugged, safe extraction and liquid chromatography?tandem mass spectrometry methodology to determine ractopamine residue in pork. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Journal of Food Protection, v. 81, n.8, p. 1258-1263, 2018. |
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DOI: |
10.4315/0362-028X.JFP-17-526 |
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Idioma: |
Inglês |
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Conteúdo: |
A new methodology is proposed for ractopamine residue analysis in pork. It consists of enzyme-mediated digestion and deconjugation steps; modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction; and liquid chromatography?tandem mass spectrometry (LC-MS/MS). In brief, the samples were digested with protease and then deconjugated with b-glucuronidase enzyme; they were then subjected to extraction and cleanup by QuEChERS and underwent sequential analysis by LC-MS/MS. The method performance was evaluated in accordance to the validation guidelines regulated by the Brazilian Ministry of Agriculture and Food Supply. The limit of detection was 0.15 lg/kg and limit of quantification was 0.5 lg/kg. When the method was applied to real samples, ractopamine residue was found in concentrations (up to 7.86 lg/kg) below international recommendation limits up to 10 lg/kg. The method is sensitive, accurate, quick, simple, and suitable for routine analysis; therefore, it is a monitoring tool that may be adopted by laboratories to achieve compliance levels. |
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Palavras-Chave: |
B-agonista; Carne suína; Method validation; Método de validação; Músculo suíno; Ractopamina; Swine muscle. |
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Thesagro: |
Carcaça; Cromatografia; Digestão; Espectrometria; Hidrolise; Nutrição Animal; Suíno. |
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Thesaurus Nal: |
Animal nutrition; Digestion; Hydrolysis; Liquid chromatography; Mass spectrometry; Methodology; Ractopamine; Swine. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02464naa a2200457 a 4500
001 2096560
005 2018-09-28
008 2018 bl uuuu u00u1 u #d
024 7 $a10.4315/0362-028X.JFP-17-526$2DOI
100 1 $aGRESSLER, V.
245 $aApplication of enzyme digestion and deconjugation followed by quick, easy, cheap, effective, rugged, safe extraction and liquid chromatography?tandem mass spectrometry methodology to determine ractopamine residue in pork.$h[electronic resource]
260 $c2018
520 $aA new methodology is proposed for ractopamine residue analysis in pork. It consists of enzyme-mediated digestion and deconjugation steps; modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction; and liquid chromatography?tandem mass spectrometry (LC-MS/MS). In brief, the samples were digested with protease and then deconjugated with b-glucuronidase enzyme; they were then subjected to extraction and cleanup by QuEChERS and underwent sequential analysis by LC-MS/MS. The method performance was evaluated in accordance to the validation guidelines regulated by the Brazilian Ministry of Agriculture and Food Supply. The limit of detection was 0.15 lg/kg and limit of quantification was 0.5 lg/kg. When the method was applied to real samples, ractopamine residue was found in concentrations (up to 7.86 lg/kg) below international recommendation limits up to 10 lg/kg. The method is sensitive, accurate, quick, simple, and suitable for routine analysis; therefore, it is a monitoring tool that may be adopted by laboratories to achieve compliance levels.
650 $aAnimal nutrition
650 $aDigestion
650 $aHydrolysis
650 $aLiquid chromatography
650 $aMass spectrometry
650 $aMethodology
650 $aRactopamine
650 $aSwine
650 $aCarcaça
650 $aCromatografia
650 $aDigestão
650 $aEspectrometria
650 $aHidrolise
650 $aNutrição Animal
650 $aSuíno
653 $aB-agonista
653 $aCarne suína
653 $aMethod validation
653 $aMétodo de validação
653 $aMúsculo suíno
653 $aRactopamina
653 $aSwine muscle
700 1 $aFEDDERN, V.
700 1 $aPEIXOTO, J. de O.
700 1 $aLEDUR, M. C.
700 1 $aDALLA COSTA, O. A.
700 1 $aLIMA, G. J. M. M. de
773 $tJournal of Food Protection$gv. 81, n.8, p. 1258-1263, 2018.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
18/03/2009 |
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Data da última atualização: |
08/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
VARGAS, S. M.; TORRES, G. A.; SOUZA SOBRINHO, F.; PEREIRA, A. V.; DAVIDE, L. C. |
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Afiliação: |
Sarah Maria Vargas, UFMG; Giovana Augusta Torres, UFLA; Fausto de Souza Sobrinho, Embrapa Gado de Leite; Antônio Vander Pereira, Embrapa Gado de Leite; Lisete Chamma Davide, UFLA. |
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Título: |
Karyotypic studies of Cratylia argentea (Desv.) O. Kuntze and C. mollis Mart. ex Benth. (Fabaceae - Papilionoideae). |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 6, n. 3. p. 707-712, 2007. |
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Idioma: |
Inglês |
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Conteúdo: |
Cratylia argentea and C. mollis (Fabaceae-Papilionoideae) are legume shrubs native to the Cerrado and Caatinga, respectively. Both species show great resistance to drought and high nutritive value, which makes them a valuable forage resource in tropical regions. Cytogenetic studies were carried out on accessions of C. argentea and C. mollis from Germplasm Banks of Embrapa Gado de Leite (Juiz de Fora, MG) and Embrapa Semi-Árido (Petrolina, PE), respectively. Root tips were treated with 3 mM 8-hydroxyquinoline and slides were made using the air-dry technique. Karyotype description for each accession took into account the following features: chromosome number; total length, relative length and arm ratio of each chromosome; haploid set length, and degree of asymmetry. Mitotic metaphases in both species showed 2n = 22 chromosomes, where this is the first report of diploid number for C. mollis. Chromosome length was also quite similar for the two species, ranging from 5.08 to 2.50 µm in C. argentea and 5.12 to 2.51 µm in C. mollis, with haploid sets of equal size, measuring 38.10 and 37.85 µm, respectively. However, they did not show the same karyotypic formula, which was 5 m + 4 sm + 2 st for C. argentea and 7 m + 2 sm + 2 st for C. mollis. This indicates the occurrence of rearrangements within chromosomes I and VI. Both karyotypes showed a tendency for asymmetry. |
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Palavras-Chave: |
Chromosome evolution; Karyotype; Tropical forage. |
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Thesagro: |
Cratylia mollis. |
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Thesaurus NAL: |
Cratylia argentea; cytogenetics. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/596285/1/Karyotypic-studies-of-Cratylia-argentea.pdf
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Marc: |
LEADER 02126naa a2200241 a 4500
001 1596285
005 2024-02-08
008 2007 bl uuuu u00u1 u #d
100 1 $aVARGAS, S. M.
245 $aKaryotypic studies of Cratylia argentea (Desv.) O. Kuntze and C. mollis Mart. ex Benth. (Fabaceae - Papilionoideae).$h[electronic resource]
260 $c2007
520 $aCratylia argentea and C. mollis (Fabaceae-Papilionoideae) are legume shrubs native to the Cerrado and Caatinga, respectively. Both species show great resistance to drought and high nutritive value, which makes them a valuable forage resource in tropical regions. Cytogenetic studies were carried out on accessions of C. argentea and C. mollis from Germplasm Banks of Embrapa Gado de Leite (Juiz de Fora, MG) and Embrapa Semi-Árido (Petrolina, PE), respectively. Root tips were treated with 3 mM 8-hydroxyquinoline and slides were made using the air-dry technique. Karyotype description for each accession took into account the following features: chromosome number; total length, relative length and arm ratio of each chromosome; haploid set length, and degree of asymmetry. Mitotic metaphases in both species showed 2n = 22 chromosomes, where this is the first report of diploid number for C. mollis. Chromosome length was also quite similar for the two species, ranging from 5.08 to 2.50 µm in C. argentea and 5.12 to 2.51 µm in C. mollis, with haploid sets of equal size, measuring 38.10 and 37.85 µm, respectively. However, they did not show the same karyotypic formula, which was 5 m + 4 sm + 2 st for C. argentea and 7 m + 2 sm + 2 st for C. mollis. This indicates the occurrence of rearrangements within chromosomes I and VI. Both karyotypes showed a tendency for asymmetry.
650 $aCratylia argentea
650 $acytogenetics
650 $aCratylia mollis
653 $aChromosome evolution
653 $aKaryotype
653 $aTropical forage
700 1 $aTORRES, G. A.
700 1 $aSOUZA SOBRINHO, F.
700 1 $aPEREIRA, A. V.
700 1 $aDAVIDE, L. C.
773 $tGenetics and Molecular Research$gv. 6, n. 3. p. 707-712, 2007.
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Embrapa Gado de Leite (CNPGL) |
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