Data/hora: 10/06/2024 18:24:23

Biblioteca(s): 	Embrapa Suínos e Aves.	
Data corrente: 	28/09/2018	
Data da última atualização: 	28/09/2018	
Autoria: 	GRESSLER, V.; FEDDERN, V.; PEIXOTO, J. de O.; LEDUR, M. C.; DALLA COSTA, O. A.; LIMA, G. J. M. M. de.	
Afiliação: 	VANESSA GRESSLER, CNPSA; VIVIAN FEDDERN, CNPSA; JANE DE OLIVEIRA PEIXOTO, CNPSA; MONICA CORREA LEDUR, CNPSA; OSMAR ANTONIO DALLA COSTA, CNPSA; GUSTAVO JULIO MELLO M DE LIMA, CNPSA.	
Título: 	Application of enzyme digestion and deconjugation followed by quick, easy, cheap, effective, rugged, safe extraction and liquid chromatography?tandem mass spectrometry methodology to determine ractopamine residue in pork.	
Ano de publicação: 	2018	
Fonte/Imprenta: 	Journal of Food Protection, v. 81, n.8, p. 1258-1263, 2018.	
DOI: 	10.4315/0362-028X.JFP-17-526	
Idioma: 	Inglês	
Conteúdo: 	A new methodology is proposed for ractopamine residue analysis in pork. It consists of enzyme-mediated digestion and deconjugation steps; modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction; and liquid chromatography?tandem mass spectrometry (LC-MS/MS). In brief, the samples were digested with protease and then deconjugated with b-glucuronidase enzyme; they were then subjected to extraction and cleanup by QuEChERS and underwent sequential analysis by LC-MS/MS. The method performance was evaluated in accordance to the validation guidelines regulated by the Brazilian Ministry of Agriculture and Food Supply. The limit of detection was 0.15 lg/kg and limit of quantification was 0.5 lg/kg. When the method was applied to real samples, ractopamine residue was found in concentrations (up to 7.86 lg/kg) below international recommendation limits up to 10 lg/kg. The method is sensitive, accurate, quick, simple, and suitable for routine analysis; therefore, it is a monitoring tool that may be adopted by laboratories to achieve compliance levels.	
Palavras-Chave: 	B-agonista; Carne suína; Method validation; Método de validação; Músculo suíno; Ractopamina; Swine muscle.	
Thesagro: 	Carcaça; Cromatografia; Digestão; Espectrometria; Hidrolise; Nutrição Animal; Suíno.	
Thesaurus Nal: 	Animal nutrition; Digestion; Hydrolysis; Liquid chromatography; Mass spectrometry; Methodology; Ractopamine; Swine.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Suínos e Aves (CNPSA)	
Exemplares: 	CNPSA - (21463-1) - UPC - AP - DD -  -  -  - DIS