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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
15/04/2004 |
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Data da última atualização: |
15/04/2004 |
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Autoria: |
PUONTI-KAERLAS, J.; LI, HQ.; WOHLWEND, H.; POTRYKUS, I. |
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Título: |
Competence for embryogenesis and organogenesis in cassava. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Revista Brasileira de Mandioca, Cruz das Almas, v. 17, p. 32, nov., 1998. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: All cassava regeneration protocols compatible with transformation require somatic embryo cultures. The competence for primary embryogenesis of a number of cassava genotypes has been assessed. The results show that in cultivars responding well to embryo induction, little improvement of the embryo induction frequency can be achieved by using meristems instead of immature leaves as starting material. On the other hand, in most recalcitrant cultivars the use of meristems allows induction of embryogenic cultures, and in many cultivars with intermediate response the efficiency can be improved by a factor of ten. Different parameters (age of donor material, medium composition, carbohydrate source, different combinations of growth regulators) of somatic embryo induction have been studied and the frequency of primary somatic embryo induction, establishment of cycling embryogenic cultures and the regeneration frequency of somatic embryos have been improved. Cycling somatic embryo cultures have been established from most cultivar, including TMS30001, which until now was considered as recalcitrant to somatic embryogenesis. The organogenesis based direct shoot regeneration system was developed to circumvent the problems sometimes encoutered when attempting plant regeneration via germinating somatic embryos. Regeneration via organogenesis from somatic embryo cotyledon explants was first optimised for one model cultivar. The organogenesis system has been further optimised and its transferability to different cultivars has so far was demonstrated with four cultivars, while others, including TMS30001 are currently investigated. MenosAbstract: All cassava regeneration protocols compatible with transformation require somatic embryo cultures. The competence for primary embryogenesis of a number of cassava genotypes has been assessed. The results show that in cultivars responding well to embryo induction, little improvement of the embryo induction frequency can be achieved by using meristems instead of immature leaves as starting material. On the other hand, in most recalcitrant cultivars the use of meristems allows induction of embryogenic cultures, and in many cultivars with intermediate response the efficiency can be improved by a factor of ten. Different parameters (age of donor material, medium composition, carbohydrate source, different combinations of growth regulators) of somatic embryo induction have been studied and the frequency of primary somatic embryo induction, establishment of cycling embryogenic cultures and the regeneration frequency of somatic embryos have been improved. Cycling somatic embryo cultures have been established from most cultivar, including TMS30001, which until now was considered as recalcitrant to somatic embryogenesis. The organogenesis based direct shoot regeneration system was developed to circumvent the problems sometimes encoutered when attempting plant regeneration via germinating somatic embryos. Regeneration via organogenesis from somatic embryo cotyledon explants was first optimised for one model cultivar. The organogenesis system has been further optimised and its... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02102naa a2200157 a 4500
001 1651905
005 2004-04-15
008 1998 bl uuuu u00u1 u #d
100 1 $aPUONTI-KAERLAS, J.
245 $aCompetence for embryogenesis and organogenesis in cassava.
260 $c1998
520 $aAbstract: All cassava regeneration protocols compatible with transformation require somatic embryo cultures. The competence for primary embryogenesis of a number of cassava genotypes has been assessed. The results show that in cultivars responding well to embryo induction, little improvement of the embryo induction frequency can be achieved by using meristems instead of immature leaves as starting material. On the other hand, in most recalcitrant cultivars the use of meristems allows induction of embryogenic cultures, and in many cultivars with intermediate response the efficiency can be improved by a factor of ten. Different parameters (age of donor material, medium composition, carbohydrate source, different combinations of growth regulators) of somatic embryo induction have been studied and the frequency of primary somatic embryo induction, establishment of cycling embryogenic cultures and the regeneration frequency of somatic embryos have been improved. Cycling somatic embryo cultures have been established from most cultivar, including TMS30001, which until now was considered as recalcitrant to somatic embryogenesis. The organogenesis based direct shoot regeneration system was developed to circumvent the problems sometimes encoutered when attempting plant regeneration via germinating somatic embryos. Regeneration via organogenesis from somatic embryo cotyledon explants was first optimised for one model cultivar. The organogenesis system has been further optimised and its transferability to different cultivars has so far was demonstrated with four cultivars, while others, including TMS30001 are currently investigated.
700 1 $aLI, HQ.
700 1 $aWOHLWEND, H.
700 1 $aPOTRYKUS, I.
773 $tRevista Brasileira de Mandioca, Cruz das Almas$gv. 17, p. 32, nov., 1998. Suplemento.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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