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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
30/05/2000 |
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Data da última atualização: |
30/05/2000 |
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Autoria: |
LESNEY, M. S. |
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Título: |
Studies on the mechanisms of plant virus infection in two leguminous protoplast systems. I. Bean pod mottle virus infection of suspension culture derived soybean protoplast. II. Infection of bean leaf protoplast with bean pod mottle and cowpea mosaic viruses. |
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Ano de publicação: |
1980 |
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Fonte/Imprenta: |
[S.l.]: Michigan State University, 1980. |
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Páginas: |
101p. |
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Idioma: |
Inglês |
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Notas: |
PhD. Thesis. |
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Conteúdo: |
Protoplasts derived from soybean ( Glycine max L. cv. 'Harosoy 63' ) liquid suspension cultures were inoculated with Bean Pod Mottle Virus ( BPMW ) in order to ascertain the effects of various inoculum amendments on the virus infection system with the ultimate goal of elucidating possible infection mechanisms. Among the parameters examined were the effects of virus concentration, protoplast concentration, buffer (pH and concentration ), poly-ornithine (PLO), calcium or magnesium chloride, and temperature on percentage infection obtained. Virus concentration effects showed a sigmoidal increase in infection with concentration increase. Protoplast concentration showed an inverse relationship to infection. Potassium phosphate buffer showed a strong optimum for infection at pH 5.6. This was used as partial evidence for the involvement of histidine in the infection process. Effects of buffer concentration were pH dependent. PLO was not required for BPMV infection, but was found to be stimulatory. A synergistic effect on increasing virus infection was demonstrated between PLO and CaCl2 at low virus concentrations. CaCl2 proved more stimulatory to BPMV infec tion than did MgCl2. Pre-incubation experiments for both buffer and the divalent cations showed that pre- incubation with the virus was nessary for most of the stimulatory effect to be obtained, but that PLO-induced infection was actually somewhat improved wheh pre-incubation was not allowed: BPMV was relatively temperature independent in the presence of inoculum amendments ( buffer, PLO, CaCl2 ), but temperature dependent in their absence; infection then was better at higher temperatures. Protoplasts were isolated from bean ( Phaseolus vulgaris L. cv. 'Pencil Pod Wax ') leaves and infected with both BPMV and cowpea mosaic virus (CPMV) in order to compare infection for the two viruses in the two different systems. The same PLO/calcium synergy was evident as in the soybean system. The optimum pH in the bean system for BPMV was 5.6. The primary difference observed between the two ystems was the ability of pH 6.0 buffer to eliminate the PLO/calcium synergy when present in the bean system, but not in the soybean system for both viruses. The similarities seen between the leaf and suspension culture protoplasts were suggestive of the latter being equally 'natural' as a system, as well as one which is more reliable. The kind and number of the complexities seen in the two virus/protoplast systems were advanced as circumstantial evidence for the possibility of receptor mediated endocytosis as the mode of infection by plant viruses in protoplasts-especially when considering such a theory as an alternative to the membrane 'wouding' hypothesis. MenosProtoplasts derived from soybean ( Glycine max L. cv. 'Harosoy 63' ) liquid suspension cultures were inoculated with Bean Pod Mottle Virus ( BPMW ) in order to ascertain the effects of various inoculum amendments on the virus infection system with the ultimate goal of elucidating possible infection mechanisms. Among the parameters examined were the effects of virus concentration, protoplast concentration, buffer (pH and concentration ), poly-ornithine (PLO), calcium or magnesium chloride, and temperature on percentage infection obtained. Virus concentration effects showed a sigmoidal increase in infection with concentration increase. Protoplast concentration showed an inverse relationship to infection. Potassium phosphate buffer showed a strong optimum for infection at pH 5.6. This was used as partial evidence for the involvement of histidine in the infection process. Effects of buffer concentration were pH dependent. PLO was not required for BPMV infection, but was found to be stimulatory. A synergistic effect on increasing virus infection was demonstrated between PLO and CaCl2 at low virus concentrations. CaCl2 proved more stimulatory to BPMV infec tion than did MgCl2. Pre-incubation experiments for both buffer and the divalent cations showed that pre- incubation with the virus was nessary for most of the stimulatory effect to be obtained, but that PLO-induced infection was actually somewhat improved wheh pre-incubation was not allowed: BPMV was relatively temperature ind... Mostrar Tudo |
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Palavras-Chave: |
Cowpea mosaic viruses; Plant diseases. |
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Thesagro: |
Doença de Planta; Glycine Max; Infecção; Protoplasto; Soja; Vírus. |
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Thesaurus Nal: |
Bean pod mottle virus; infection; plant viruses; protoplasts; soybeans. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03657nam a2200289 a 4500
001 1559934
005 2000-05-30
008 1980 bl uuuu m 00u1 u #d
100 1 $aLESNEY, M. S.
245 $aStudies on the mechanisms of plant virus infection in two leguminous protoplast systems. I. Bean pod mottle virus infection of suspension culture derived soybean protoplast. II. Infection of bean leaf protoplast with bean pod mottle and cowpea mosaic viruses.
260 $a[S.l.]: Michigan State University$c1980
300 $a101p.
500 $aPhD. Thesis.
520 $aProtoplasts derived from soybean ( Glycine max L. cv. 'Harosoy 63' ) liquid suspension cultures were inoculated with Bean Pod Mottle Virus ( BPMW ) in order to ascertain the effects of various inoculum amendments on the virus infection system with the ultimate goal of elucidating possible infection mechanisms. Among the parameters examined were the effects of virus concentration, protoplast concentration, buffer (pH and concentration ), poly-ornithine (PLO), calcium or magnesium chloride, and temperature on percentage infection obtained. Virus concentration effects showed a sigmoidal increase in infection with concentration increase. Protoplast concentration showed an inverse relationship to infection. Potassium phosphate buffer showed a strong optimum for infection at pH 5.6. This was used as partial evidence for the involvement of histidine in the infection process. Effects of buffer concentration were pH dependent. PLO was not required for BPMV infection, but was found to be stimulatory. A synergistic effect on increasing virus infection was demonstrated between PLO and CaCl2 at low virus concentrations. CaCl2 proved more stimulatory to BPMV infec tion than did MgCl2. Pre-incubation experiments for both buffer and the divalent cations showed that pre- incubation with the virus was nessary for most of the stimulatory effect to be obtained, but that PLO-induced infection was actually somewhat improved wheh pre-incubation was not allowed: BPMV was relatively temperature independent in the presence of inoculum amendments ( buffer, PLO, CaCl2 ), but temperature dependent in their absence; infection then was better at higher temperatures. Protoplasts were isolated from bean ( Phaseolus vulgaris L. cv. 'Pencil Pod Wax ') leaves and infected with both BPMV and cowpea mosaic virus (CPMV) in order to compare infection for the two viruses in the two different systems. The same PLO/calcium synergy was evident as in the soybean system. The optimum pH in the bean system for BPMV was 5.6. The primary difference observed between the two ystems was the ability of pH 6.0 buffer to eliminate the PLO/calcium synergy when present in the bean system, but not in the soybean system for both viruses. The similarities seen between the leaf and suspension culture protoplasts were suggestive of the latter being equally 'natural' as a system, as well as one which is more reliable. The kind and number of the complexities seen in the two virus/protoplast systems were advanced as circumstantial evidence for the possibility of receptor mediated endocytosis as the mode of infection by plant viruses in protoplasts-especially when considering such a theory as an alternative to the membrane 'wouding' hypothesis.
650 $aBean pod mottle virus
650 $ainfection
650 $aplant viruses
650 $aprotoplasts
650 $asoybeans
650 $aDoença de Planta
650 $aGlycine Max
650 $aInfecção
650 $aProtoplasto
650 $aSoja
650 $aVírus
653 $aCowpea mosaic viruses
653 $aPlant diseases
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Registro original: |
Embrapa Cerrados (CPAC) |
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Biblioteca |
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Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
31/10/2012 |
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Data da última atualização: |
27/06/2019 |
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Tipo da produção científica: |
Orientação de Tese de Pós-Graduação |
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Autoria: |
LIMA, A. R. A. |
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Afiliação: |
ÂNGELA ROBERTA ALVES LIMA, UFPel. |
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Título: |
Agricultoras no cuidado da família com uso das plantas medicinais. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
2012. |
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Páginas: |
115 f. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Notas: |
Dissertação (Mestrado em Enfermagem) - Faculdade de Enfermagem, Universidade Federal de Pelotas, orientadora Rita Maria Heck: UFPel; Coorientadora
Rosa Lia Barbieri; Embrapa Clima Temperado. |
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Palavras-Chave: |
Cuidado familiar; Cultura; Enfermagem rural; Plantas medicinais; Saúde da família. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/69093/1/Dissertacao.pdf
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Marc: |
LEADER 00668nam a2200181 a 4500
001 1938582
005 2019-06-27
008 2012 bl uuuu m 00u1 u #d
100 1 $aLIMA, A. R. A.
245 $aAgricultoras no cuidado da família com uso das plantas medicinais.
260 $a2012.$c2012
300 $a115 f.$c1 CD-ROM.
500 $aDissertação (Mestrado em Enfermagem) - Faculdade de Enfermagem, Universidade Federal de Pelotas, orientadora Rita Maria Heck: UFPel; Coorientadora Rosa Lia Barbieri; Embrapa Clima Temperado.
653 $aCuidado familiar
653 $aCultura
653 $aEnfermagem rural
653 $aPlantas medicinais
653 $aSaúde da família
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