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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
30/08/2018 |
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Data da última atualização: |
30/08/2018 |
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Autoria: |
SANTOS, A. C.; LEITE, F. P. L.; VIANNA, A. M.; WEEGE, G. B.; FINGER, I. S.; MULLER, V.; CURCIO, B. R.; NOGUEIRA, C. E. W. |
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Afiliação: |
Alice C. Santos, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Fábio P. L. Leite, Centro de Desenvolvimento Tecnológico - UFPel; Ana M. Vianna, Centro de Desenvolvimento Tecnológico - UFPel; Guilherme B. Weege, Laboratório de Parasitologia Molecular e Imunologia - UFPel; Ilusca S. Finger, Centro de Desenvolvimento Tecnológico - UFPel; Vitória Müller, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Bruna R. Curcio, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Carlos E. W. Nogueira, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel. |
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Título: |
Dynamics of humoral immune response in pregnant mares and foals vaccinated with Theileria equi recombinant EMA-2. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 6, p. 1105-1109, junho 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Dinâmica da resposta imune humoral em éguas gestantes e potros vacinados com EMA-2 recombinante de Theileria equi. |
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Conteúdo: |
Theileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares presented antibody levels of 1.0432±0.33. Foals delivered by vaccinated mares presented levels of antibodies greater than those of foals delivered by control mares after their first time sucking (at about twelve hours after birth). Foals vaccinated in the second month of life showed an 8.3-fold increase in antibody levels when compared to baseline values. The vaccination schedule with rEMA-2 was able to stimulate humoral immunity in pregnant mares. Vaccine immunoglobins were concentrated in the colostrum of vaccinated mares and foals delivered by these mares showed an increase in serum levels of vaccine antibodies after the first-time sucking. MenosTheileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares prese... Mostrar Tudo |
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Palavras-Chave: |
Hemoprotozoan; Hemoprotozoário; Imunidade humoral; Piroplasmose equina; REMA-2. |
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Thesagro: |
Eqüino. |
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Thesaurus Nal: |
Equine piroplasmosis; Horses; Humoral immunity; Parasitoses; Theileria equi. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182208/1/Dynamics-of-humoral-immune-response-in-pregnant-mares.pdf
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Marc: |
LEADER 03294naa a2200349 a 4500
001 2094866
005 2018-08-30
008 2018 bl uuuu u00u1 u #d
100 1 $aSANTOS, A. C.
245 $aDynamics of humoral immune response in pregnant mares and foals vaccinated with Theileria equi recombinant EMA-2.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Dinâmica da resposta imune humoral em éguas gestantes e potros vacinados com EMA-2 recombinante de Theileria equi.
520 $aTheileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares presented antibody levels of 1.0432±0.33. Foals delivered by vaccinated mares presented levels of antibodies greater than those of foals delivered by control mares after their first time sucking (at about twelve hours after birth). Foals vaccinated in the second month of life showed an 8.3-fold increase in antibody levels when compared to baseline values. The vaccination schedule with rEMA-2 was able to stimulate humoral immunity in pregnant mares. Vaccine immunoglobins were concentrated in the colostrum of vaccinated mares and foals delivered by these mares showed an increase in serum levels of vaccine antibodies after the first-time sucking.
650 $aEquine piroplasmosis
650 $aHorses
650 $aHumoral immunity
650 $aParasitoses
650 $aTheileria equi
650 $aEqüino
653 $aHemoprotozoan
653 $aHemoprotozoário
653 $aImunidade humoral
653 $aPiroplasmose equina
653 $aREMA-2
700 1 $aLEITE, F. P. L.
700 1 $aVIANNA, A. M.
700 1 $aWEEGE, G. B.
700 1 $aFINGER, I. S.
700 1 $aMULLER, V.
700 1 $aCURCIO, B. R.
700 1 $aNOGUEIRA, C. E. W.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 6, p. 1105-1109, junho 2018
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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| Registros recuperados : 25 | |
| 3. |  | SANTOS JUNIOR, A. G.; CUNHA, R. C.; ANDREOTTI, R.; LEITE, F. P. L. Avaliação de diferentes métodos de purificação da proteína bm86-cg expressa em pichia pastoris. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., 2010, Campo Grande, MS. [Anais...]. Campo Grande, MS: Colégio Brasileiro de Parasitologia Veterinária, 2010. 1 CD-ROM. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 4. | | ANDREOTTI, R.; SAIMO-KAHWA, M.; CUNHA, R. C.; BARROS, J. C.; LEITE, F. P. L. Characterization and Production of recombinant protein Ra92A from Rhipicephalus appendiculatus aiming tick vaccine antigen in Uganda. In: INTERNATIONAL CONGRESS OF PARASITOLOGY, 13., 2014, Mexico. Conference abstracts... Mexico City, Mexico: Academic Committee ICOPA Congress, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
|  |
| 8. | | CUNHA, R. C.; LEITE, F. P. L.; PINTO, L. da S.; SATO, T.; ANDREOTTI, R. Subclonagem do gene Bm86-CG em pPIC9 para expressão heteróloga em Pichia pastoris. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA 21.; ENCONTRO DE PARASITOLOGIA DO MERCOSUL, 2., 2009, Foz do Iguaçu. Novos horizontes em parasitologia: anais. Foz do Iguaçu: SBP, 2009. p. 1298. Revista Patologia Tropical, v. 38, jul.-set 2009. Suplemento 2.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 9. | | CUNHA, R. C.; PÉREZ DE LEÓN, A. A.; LEITE, F. P. L.; GUERRERO, F. D.; PINTO, L. S.; ANDREOTTI, R. Assessment of bovine immunoprotection against Rhipicephalus microplus using Bm86-CG antigen expressed in Pichia pastoris. In: AMERICAN ASSOCIATION OF VETERINARY PARASITOLOGISTS ANNUAL MEETING, 56.; LIVESTOCK INSECT WORKERS CONFERENCE, 55.; INTERNATIONAL SYMPOSIUM ON ECTOPARASITES OF PETS, 11.,2011,ST. LOUIS, MO. Sustainable parasite and vector control: proceedings. St. Louis,MO: American Association of Veterinary Parasitologists, 2011. p.95| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 10. | | PINHEIRO, A. F.; MADRI, I. M.; BORSUK, S.; ANDREOTTI, R.; BERME, M. E. A.; LEITE, F. P. L. The adjuvant immune modulation effect in experimental vaccine against Neospora caninum using rNP43 expressed in Pichia pastoris. In: INTERNATIONAL MEETING ON APICOMPLEXAN PARASITES IN FARM ANIMALS, 2012, Lisbon. Apicomplexa in farm animals: proceedings. Lisboa: Escola Superior de Tecnologia da Saúde de Lisboa , 2012. p.131 Poster 66, Apicowplexa2012| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 11. | | ANDREOTTI, R.; MATOS, M. de F. C.; PAIVA, F.; PELLEGRIN, A. O.; LEITE, F. P. L.; VINCENT, A.; KREUTZ, F. Desenvolvimento de métodos de diagnóstico, controle e avaliação epidemiológico da neosporose. In: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. Anais... Campo Grande, MS: Embrapa Gado de Corte, 2009. 2 p. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 12. | | PINTO, N. P.; MINHO, A. P.; GASPAR, E. B.; DOMINGUES, R.; MOURA, M. Q. de; LEITE, F. P. L. Controle de Haemonchus contortus em ovinos utilizando Saccharomyces cerevisiae. In: ENCONTRO DE PÓS-GRADUAÇÃO DA UFPEL, 19., 2017, Pelotas, RS. Anais... Pelota, RS: UFPel, 2017. p. 1-4.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 14. | | PINHEIRO, A. F.; ROLOFF, B. C.; MOREIRA, A. da S.; BERNE, M. E. A.; ANDREOTTI, R.; LEITE, F. P. L. Identification of suitable adjuvant for vaccine formulation with the Neospora caninum antigen NcSRS2. Vaccine, v. 36, n. 9, p. 1154-1159, February 2018| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 15. | | ANDREOTTI, R.; PRATA, M. C. de A.; GUIMARAES, M. F. M.; LÉON, A. P. de; MELO, A. K. M.; LEITE, F. P. L. Abordagem molecular no diagnóstico da resistência e no controle do carrapato-do-boi. In: SIMPÓSIO EMBRAPA LABEX DE SANIDADE ANIMAL, 1., 2009, Campo Grande, MS. Anais... Campo Grande, MS: Embrapa Gado de Corte, 2009. 2 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 16. | | SANTOS JUNIOR, A. G. dos; PIRAINE, R. E. A.; CUNHA, R. C.; NIZOLI, L. Q.; ANDREOTTI, R.; LEITE, F. P. L. Avaliação de métodos para obtenção de proteínas recombinantes. Science and Animal Health, v.5, n.2, p. 166-177, maio/ago. 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 17. | | RODRIGUES, S. C.; LUCAS, C.; BORSUK, S.; FERNANDES, C. H.; ANDREOTTI, R.; LEITE, F. P. L.; OSHIRO, L. M.; MATOS, M. de F. C.; BERNE, M. E. A. Desenvolvimento de um ELISA para diagnóstico de Neospora caninum em bovinos utilizando NCSR2 recombinante. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA 21.; ENCONTRO DE PARASITOLOGIA DO MERCOSUL, 2., 2009, Foz do Iguaçu. Novos horizontes em parasitologia: anais. Foz do Iguaçu: SBP, 2009. p. 200. Revista Patologia Tropical, v. 38, jul.-set 2009. Suplemento 2.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 18. | | RODRIGUES, S. C.; LUCAS, C.; BORSUK, S.; FERNANDES, C. H.; LEITE, F. P. L.; PINTO, L. da S.; ANDREOTTI, R.; BERNE, M. E. A. Desenvolvimento de vacinas recombinantes para o controle de Neospora caninum. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA 21.; ENCONTRO DE PARASITOLOGIA DO MERCOSUL, 2., 2009, Foz do Iguaçu. Novos horizontes em parasitologia: anais. Foz do Iguaçu: SBP, 2009. p. 122. Revista Patologia Tropical, v. 38, jul.-set 2009. Suplemento 2.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 19. | | CUNHA, R. C.; LEÓN, A. A. P. de; LEITE, F. P. L.; PINTO, L. da S.; SANTOS JÚNIOR, A. G. dos; SILVA, R. A. e. Bovine immunoprotection against Rhipicephalus (Boophilus) microplus with recombinant Bm86-Campo Grande antigen. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 21, n. 3, p. 1-9, jul.-set. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 20. | | PINTO, N. B.; GASPAR, E. B.; MINHO, A. P.; MOURA, M. Q. de; CAPELLA, G. de A.; LEITE, F. P. L. Redução da prolificidade de Haemonchus contortus em ovinos estimulados por levedura. In: ENCONTRO DE PÓS-GRADUAÇÃO, 21.; SEMANA INTEGRADA, 5., 2019, Pelotas. Anais... Pelotas: UFPel, 2019. ENPOS.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| Registros recuperados : 25 | |
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