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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Solos. |
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Data corrente: |
06/02/2015 |
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Data da última atualização: |
06/02/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LEITE, D. C. A.; BALIEIRO, F. C.; C. A. PIRES; MADARI, B. E.; ROSADO, A. S.; COUTINHO, H. L. C.; PEIXOTO, R. S. |
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Afiliação: |
D. C. A. LEITE, UFRJ; FABIANO DE CARVALHO BALIEIRO, CNPS; C. A. PIRES; BEATA EMOKE MADARI, CNPAF; A. S. ROSADO, UFRJ; HEITOR LUIZ DA COSTA COUTINHO, CNPS; R. S. PEIXOTO, UFRJ. |
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Título: |
Comparison of DNA extraction protocols for microbial communities from soil treated with biochar. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 45, n. 1, p. 175-183, 2014. |
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DOI: |
10.1590/S1517-83822014000100023 |
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Idioma: |
Inglês |
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Conteúdo: |
Many studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercialDNAextraction kits, the FastDNA® SPIN Kit for Soil (FD kit), the PowerSoil® DNA Isolation Kit (PS kit) and the ZR Soil Microbe DNA Kit MiniprepTM (ZR kit), for extracting microbial genomicDNAfrom sand treated with different types of biochar. The methods were evaluated by comparing the DNA yields and purity and by analysing the bacterial and fungal community profiles generated by PCR-DGGE. Our results showed that the PCR-DGGE profiles for bacterial and fungal communities were highly affected by the purity and yield of the different DNA extracts. Among the tested kits, the PS kit was the most efficient with respect to the amount and purity of recovered DNA and considering the complexity of the generated DGGE microbial fingerprint from the sand-biochar samples. MenosMany studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercialDNAextraction kits, the FastDNA® SPIN Kit for Soil (FD kit), the PowerSoil® DNA Isolation Kit (PS kit) and the ZR Soil Microbe DNA Kit MiniprepTM (ZR kit), for extracting microbial genomicDNAfrom sand treated with different types of biochar. The methods were evaluated by comparing the DNA yields and purity and by analysing the bacterial and fungal community profiles generated by PCR-DGGE. Our results showed that the PCR-DGGE profiles for bacterial and fungal communities were highly affected by the purity and yield of the different DNA extracts. Among the tested kits, the PS kit was the most efficient with respect to the amount and purity of recovered DNA and considering the complexity of the generated DGGE microbial fingerprint from the sand-... Mostrar Tudo |
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Palavras-Chave: |
Comunidades microbianas; Extração de DNA; Índice de pureza do DNA; PCR-DGGE. |
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Thesaurus Nal: |
biochar. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/117433/1/BJMv45n1a23.pdf
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Marc: |
LEADER 02342naa a2200265 a 4500
001 2007986
005 2015-02-06
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1590/S1517-83822014000100023$2DOI
100 1 $aLEITE, D. C. A.
245 $aComparison of DNA extraction protocols for microbial communities from soil treated with biochar.$h[electronic resource]
260 $c2014
520 $aMany studies have evaluated the effects of biochar application on soil structure and plant growth. However, there are very few studies describing the effect of biochar on native soil microbial communities. Microbial analysis of environmental samples requires accurate and reproducible methods for the extraction of DNA from samples. Because of the variety among microbial species and the strong adsorption of the phosphate backbone of the DNA molecule to biochar, extracting and purifying high quality microbial DNA from biochar-amended soil is not a trivial process and can be considerably more difficult than the extraction of DNA from other environmental samples. The aim of this study was to compare the relative efficacies of three commercialDNAextraction kits, the FastDNA® SPIN Kit for Soil (FD kit), the PowerSoil® DNA Isolation Kit (PS kit) and the ZR Soil Microbe DNA Kit MiniprepTM (ZR kit), for extracting microbial genomicDNAfrom sand treated with different types of biochar. The methods were evaluated by comparing the DNA yields and purity and by analysing the bacterial and fungal community profiles generated by PCR-DGGE. Our results showed that the PCR-DGGE profiles for bacterial and fungal communities were highly affected by the purity and yield of the different DNA extracts. Among the tested kits, the PS kit was the most efficient with respect to the amount and purity of recovered DNA and considering the complexity of the generated DGGE microbial fingerprint from the sand-biochar samples.
650 $abiochar
653 $aComunidades microbianas
653 $aExtração de DNA
653 $aÍndice de pureza do DNA
653 $aPCR-DGGE
700 1 $aBALIEIRO, F. C.
700 1 $aC. A. PIRES
700 1 $aMADARI, B. E.
700 1 $aROSADO, A. S.
700 1 $aCOUTINHO, H. L. C.
700 1 $aPEIXOTO, R. S.
773 $tBrazilian Journal of Microbiology, São Paulo$gv. 45, n. 1, p. 175-183, 2014.
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Registro original: |
Embrapa Solos (CNPS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
01/04/2013 |
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Data da última atualização: |
01/04/2013 |
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Tipo da produção científica: |
Documentos |
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Autoria: |
SILVA, A. F.; SILVA, M. M. da; SANTANA, L. M. de; MOREIRA, A. N.; PEREIRA, J. R. B.; AMORIM, S. A. S.; FRANÇA, C. R. R. S.; SANTOS, A. P. G.; MELO, R, B. de. |
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Afiliação: |
ALINEAUREA FLORENTINO SILVA, CPATSA; MAIRON MOURA DA SILVA; LUIZ MANOEL DE SANTANA; ANDRÉA NUNES MOREIRA; JOSÉ ROBERTO BRITO PEREIRA; SEBASTIÃO ANTÔNIO SANTOS AMORIM; CARLA REGINE REGES SILVA FRANÇA; ANA PAULA GUIMARÃES SANTOS; ROSEMARY BARBOSA DE MELO. |
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Título: |
Controle alternativo do moleque-da-bananeira e manejo de hortas agroecológicas em comunidades do Semiárido nordestino. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Petrolina: Embrapa Semiárido, 2012. |
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Páginas: |
44 p. |
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Série: |
(Embrapa Semiárido. Documentos, 252). |
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Idioma: |
Português |
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Conteúdo: |
Campo de pesquisa participativa do Projeto Maria Tereza; Campo de pesquisa participativa do Núcleo 4 do Projeto de Irrigação Senador Nilo Coelho; Campo de aprendizagem tecnológica de horta agroecológica comunitária em Nova Descoberta, Petrolina, PE; Campo de pesquisa participativa sobre a permanência das características químicas do biofertilizante vairo preparado na comunidade de Nova Descoberta; Campo de pesquisa participativa sobre a adaptação de diferentes variedades de alface em cultivo agroecológico na comunidade de Nova Descoberta; campo de aprendizagem tecnológica: Comportamento econômico e produtivo da horta comunitária agroecológica de Nova Descoberta; Campo de pesquisa participativa de cultivo de diferentes espécies em horta agroecológica comunitária com a utilização de água de poço. |
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Palavras-Chave: |
Disease; Horta agroecológica; Moleque-da-bananeira; Semiárido. |
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Thesagro: |
Alface; Banana; Coentro; Cosmopolites Sordidus; Doença; Horticultura; Manejo; Pimentão; Praga de Planta. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/80053/1/SDC252.pdf
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Marc: |
LEADER 01916nam a2200385 a 4500
001 1954583
005 2013-04-01
008 2012 bl uuuu u0uu1 u #d
100 1 $aSILVA, A. F.
245 $aControle alternativo do moleque-da-bananeira e manejo de hortas agroecológicas em comunidades do Semiárido nordestino.$h[electronic resource]
260 $aPetrolina: Embrapa Semiárido$c2012
300 $a44 p.
490 $a(Embrapa Semiárido. Documentos, 252).
520 $aCampo de pesquisa participativa do Projeto Maria Tereza; Campo de pesquisa participativa do Núcleo 4 do Projeto de Irrigação Senador Nilo Coelho; Campo de aprendizagem tecnológica de horta agroecológica comunitária em Nova Descoberta, Petrolina, PE; Campo de pesquisa participativa sobre a permanência das características químicas do biofertilizante vairo preparado na comunidade de Nova Descoberta; Campo de pesquisa participativa sobre a adaptação de diferentes variedades de alface em cultivo agroecológico na comunidade de Nova Descoberta; campo de aprendizagem tecnológica: Comportamento econômico e produtivo da horta comunitária agroecológica de Nova Descoberta; Campo de pesquisa participativa de cultivo de diferentes espécies em horta agroecológica comunitária com a utilização de água de poço.
650 $aAlface
650 $aBanana
650 $aCoentro
650 $aCosmopolites Sordidus
650 $aDoença
650 $aHorticultura
650 $aManejo
650 $aPimentão
650 $aPraga de Planta
653 $aDisease
653 $aHorta agroecológica
653 $aMoleque-da-bananeira
653 $aSemiárido
700 1 $aSILVA, M. M. da
700 1 $aSANTANA, L. M. de
700 1 $aMOREIRA, A. N.
700 1 $aPEREIRA, J. R. B.
700 1 $aAMORIM, S. A. S.
700 1 $aFRANÇA, C. R. R. S.
700 1 $aSANTOS, A. P. G.
700 1 $aMELO, R, B. de
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Registro original: |
Embrapa Semiárido (CPATSA) |
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