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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
24/01/2000 |
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Data da última atualização: |
24/01/2000 |
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Autoria: |
LEE, R. F. |
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Afiliação: |
University of Florida, IFAS, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred. |
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Título: |
Use of double stranded RNAs to diagnose citrus tristeza virus strains. |
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Ano de publicação: |
1984 |
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Fonte/Imprenta: |
Proc. Ann. Meet. Fla. State Hort. Soc., v.97, p.53-56, 1984. |
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Idioma: |
Inglês |
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Conteúdo: |
Double stranded ribonucleic acids (dsRNAs) were examined as a possible way to diagnose citrus tristeza virus (CTV) strains. The dsRNAs were purified from CTV-infected bark tissue by the use of CF-11 cellulose followed by polyacrylamide gel electrophoresis. The sizes of dsRNAs purified from 5 CTV isolates which differed widely in their biological activity were compared. Each CTV isolate had a 13 X 10 dalton dsRNA corresponding to the reported fulllength replicaative form as well as several shorter (subgenomic) dsRNAs. The number and sizes of the subgenomic dsRNAs for each CTV isolate were unique from each host but seemed to be affected by the citrus cultivar used as the host. |
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Palavras-Chave: |
Diagnosis; Diseases; Doencas; Orange. |
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Thesagro: |
Diagnostico; Laranja; Vírus. |
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Categoria do assunto: |
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Marc: |
LEADER 01207naa a2200205 a 4500
001 1648599
005 2000-01-24
008 1984 bl --- 0-- u #d
100 1 $aLEE, R. F.
245 $aUse of double stranded RNAs to diagnose citrus tristeza virus strains.
260 $c1984
520 $aDouble stranded ribonucleic acids (dsRNAs) were examined as a possible way to diagnose citrus tristeza virus (CTV) strains. The dsRNAs were purified from CTV-infected bark tissue by the use of CF-11 cellulose followed by polyacrylamide gel electrophoresis. The sizes of dsRNAs purified from 5 CTV isolates which differed widely in their biological activity were compared. Each CTV isolate had a 13 X 10 dalton dsRNA corresponding to the reported fulllength replicaative form as well as several shorter (subgenomic) dsRNAs. The number and sizes of the subgenomic dsRNAs for each CTV isolate were unique from each host but seemed to be affected by the citrus cultivar used as the host.
650 $aDiagnostico
650 $aLaranja
650 $aVírus
653 $aDiagnosis
653 $aDiseases
653 $aDoencas
653 $aOrange
773 $tProc. Ann. Meet. Fla. State Hort. Soc.$gv.97, p.53-56, 1984.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | AZEVEDO, F. A. de; MOURÃO FILHO, F. de A. A.; SCHINOR, E. H.; PAOLI, L. G. de; MENDES, B. M. J.; HARAKAVA, R.; GABRIEL, D. W.; LEE, R. F. GUS gene expression driven by a citrus promoter in transgenic tobacco and 'Valencia' sweet orange. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 41, n. 11, p. 1623-1628, nov. 2006 Título em português: Expressão do gene GUS controlado por promotor de citros em plantas transgênicas de tabaco e laranja 'Valência'.| Biblioteca(s): Embrapa Unidades Centrais. |
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