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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
16/09/1993 |
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Data da última atualização: |
16/09/1993 |
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Autoria: |
ROCHA-PENA, M. A.; LEE, R. F.; PERMAR, T. A.; YOKOMI, R. K.; GARNSEY, S. M. |
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Afiliação: |
Univ. Florida, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA. |
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Título: |
Use of enzyme-linked immunosorbent and dot-immunobinding assays to evaluate two mild-strain cross protection experiments after challenge with a severe citrus tristeza virus isolate. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Riverside, California: International Organization of Citrus Virologists, 1991. |
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Páginas: |
93-102 |
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Idioma: |
Inglês |
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Notas: |
Conference of the International Organization of Citrus Virologists, 11., 1991, Riverside, California, Proceedings... |
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Conteúdo: |
In two experiments citrus trees on sour orange rootstock were pre-inoculated with 14 mild citrus tristeza virus (CTV) isolates and were then graft or aphid challenged with a severe quick decline CTV isolate after the trees were stablished in the field. Double antibody sandwich ELISA (DAS-ELISA) with polyclonal antibodies was used to detect CTV infection, and DAS-indirect ELISA using MCA-13 monoclonal antibody was used to detect severe CTV isolates. All trees in the two experiments were surveyed in August and November, 1989. DAS-ELISA in the August survey showed all the pre-inoculated plants to be infected with CTV, and only one plant in the aphid challenged experiment was positive when assayed with DAS-indirect ELISA using the MCA-13 monoclonal antibody. In N ovember, 1989, six addittional plants were positive when assayed by DAS-indirect ELISA with MCA-13. Some originally helthy uninoculated control plants were found to be infect by DAS-ELISA but not by DAS-indirect ELISA, indicating that some natural spread of mild CTV isolates had occured in both experiments. A dot-immunobinding assay was compared to DAS-ELISA and DAS-indirect ELISA and found to be more rapid and as sensitive in detection of CTV in crude preparations. The absence of reaction of the MCA-13 monoclonal antibody in both DAS-indirect ELISA and dot-immunobinding assay on most of the plants previously challenged with the severe CTV isolate suggests that the mild CTV isolates may by providing cross protection against the severe challenge isolates. MenosIn two experiments citrus trees on sour orange rootstock were pre-inoculated with 14 mild citrus tristeza virus (CTV) isolates and were then graft or aphid challenged with a severe quick decline CTV isolate after the trees were stablished in the field. Double antibody sandwich ELISA (DAS-ELISA) with polyclonal antibodies was used to detect CTV infection, and DAS-indirect ELISA using MCA-13 monoclonal antibody was used to detect severe CTV isolates. All trees in the two experiments were surveyed in August and November, 1989. DAS-ELISA in the August survey showed all the pre-inoculated plants to be infected with CTV, and only one plant in the aphid challenged experiment was positive when assayed with DAS-indirect ELISA using the MCA-13 monoclonal antibody. In N ovember, 1989, six addittional plants were positive when assayed by DAS-indirect ELISA with MCA-13. Some originally helthy uninoculated control plants were found to be infect by DAS-ELISA but not by DAS-indirect ELISA, indicating that some natural spread of mild CTV isolates had occured in both experiments. A dot-immunobinding assay was compared to DAS-ELISA and DAS-indirect ELISA and found to be more rapid and as sensitive in detection of CTV in crude preparations. The absence of reaction of the MCA-13 monoclonal antibody in both DAS-indirect ELISA and dot-immunobinding assay on most of the plants previously challenged with the severe CTV isolate suggests that the mild CTV isolates may by providing cross protection aga... Mostrar Tudo |
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Palavras-Chave: |
Citrus excelsa. |
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Thesagro: |
Doença; Elisa; Pomelo. |
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Thesaurus Nal: |
blight; Florida. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02442naa a2200265 a 4500
001 1644224
005 1993-09-16
008 1991 bl uuuu u00u1 u #d
100 1 $aROCHA-PENA, M. A.
245 $aUse of enzyme-linked immunosorbent and dot-immunobinding assays to evaluate two mild-strain cross protection experiments after challenge with a severe citrus tristeza virus isolate.
260 $c1991
300 $a93-102
500 $aConference of the International Organization of Citrus Virologists, 11., 1991, Riverside, California, Proceedings...
520 $aIn two experiments citrus trees on sour orange rootstock were pre-inoculated with 14 mild citrus tristeza virus (CTV) isolates and were then graft or aphid challenged with a severe quick decline CTV isolate after the trees were stablished in the field. Double antibody sandwich ELISA (DAS-ELISA) with polyclonal antibodies was used to detect CTV infection, and DAS-indirect ELISA using MCA-13 monoclonal antibody was used to detect severe CTV isolates. All trees in the two experiments were surveyed in August and November, 1989. DAS-ELISA in the August survey showed all the pre-inoculated plants to be infected with CTV, and only one plant in the aphid challenged experiment was positive when assayed with DAS-indirect ELISA using the MCA-13 monoclonal antibody. In N ovember, 1989, six addittional plants were positive when assayed by DAS-indirect ELISA with MCA-13. Some originally helthy uninoculated control plants were found to be infect by DAS-ELISA but not by DAS-indirect ELISA, indicating that some natural spread of mild CTV isolates had occured in both experiments. A dot-immunobinding assay was compared to DAS-ELISA and DAS-indirect ELISA and found to be more rapid and as sensitive in detection of CTV in crude preparations. The absence of reaction of the MCA-13 monoclonal antibody in both DAS-indirect ELISA and dot-immunobinding assay on most of the plants previously challenged with the severe CTV isolate suggests that the mild CTV isolates may by providing cross protection against the severe challenge isolates.
650 $ablight
650 $aFlorida
650 $aDoença
650 $aElisa
650 $aPomelo
653 $aCitrus excelsa
700 1 $aLEE, R. F.
700 1 $aPERMAR, T. A.
700 1 $aYOKOMI, R. K.
700 1 $aGARNSEY, S. M.
773 $tRiverside, California: International Organization of Citrus Virologists, 1991.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | AZEVEDO, F. A. de; MOURÃO FILHO, F. de A. A.; SCHINOR, E. H.; PAOLI, L. G. de; MENDES, B. M. J.; HARAKAVA, R.; GABRIEL, D. W.; LEE, R. F. GUS gene expression driven by a citrus promoter in transgenic tobacco and 'Valencia' sweet orange. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 41, n. 11, p. 1623-1628, nov. 2006 Título em português: Expressão do gene GUS controlado por promotor de citros em plantas transgênicas de tabaco e laranja 'Valência'.| Biblioteca(s): Embrapa Unidades Centrais. |
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