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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
16/09/1993 |
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Data da última atualização: |
16/09/1993 |
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Autoria: |
YOKOMI, R. K.; GARNSEY, S. M.; PERMAR, T. A.; LEE, R. F.; YOUTSEY, C. O. |
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Afiliação: |
USDA, ARS, 2120 Camden Road, Orlando, FL 32803, USA. |
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Título: |
Natural spread of severe citrus tristeza virus isolates in citrus preinfected with mild CTV isolates. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Riverside, California: International Organization of Citrus Virologists, 1991. |
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Páginas: |
p.86-92 |
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Idioma: |
Inglês |
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Notas: |
Conference of the International Organization of Citrus virologists, 11., 1991, Riverside, California, Proceedings... |
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Conteúdo: |
Natural spread of severe citrus tristeza virus (CTV) isolates was monitored in a 2-ha, 306-tree plot near Dundee, Florida. This plot, planted in spring 1986, consisted of Hamlin orange and Redblush grapefruit trees on sour orange rootstock preinfected with fourteen different CTV mild isolates. The trees were surveyed for symptoms of CTV decline in November 1988, March 1989, and October 1989, and assayed by enzyme-linked immunosorbent assay (ELISA). A monoclonal antibody, CTV-MCA13, which reacts specifically to severe decline-inducing isolates of CTV, and a broad-spectrum monoclonal antibody, 3DF1, were used in double sandwich indirect assay. After 3 yr of natural challenge, 3DF1-based ELISA indicated that 10 of 18 (56%) Hamlin and four of 19 (21%) Redblush grapefruit control trees became infected with CTV; seven of these Hamlin and all Redblush trees naturally infected with CTV also reacted with CTV-MCA13, indicating that they were infected with a severe isolate. Infection levels of CTV severe isolates in preinfected Hamlin treatments ranged from 0 to 50% based on CTV-MCA13 ELISA, with two treatments free of severe isolates. In Redblush grapefruit, infection levels of CTV severe isolates ranged from 0 to 22% but 11 CTV mild isolate treatments remained free from severe CTV infection. Decline symptoms were observed in 34 Hamlin and six grapefruit trees and supported the CTV-MCA13 ELISA results. Although substantial differences occurred between Hamlin treatments, results were not statistically different due to the ... MenosNatural spread of severe citrus tristeza virus (CTV) isolates was monitored in a 2-ha, 306-tree plot near Dundee, Florida. This plot, planted in spring 1986, consisted of Hamlin orange and Redblush grapefruit trees on sour orange rootstock preinfected with fourteen different CTV mild isolates. The trees were surveyed for symptoms of CTV decline in November 1988, March 1989, and October 1989, and assayed by enzyme-linked immunosorbent assay (ELISA). A monoclonal antibody, CTV-MCA13, which reacts specifically to severe decline-inducing isolates of CTV, and a broad-spectrum monoclonal antibody, 3DF1, were used in double sandwich indirect assay. After 3 yr of natural challenge, 3DF1-based ELISA indicated that 10 of 18 (56%) Hamlin and four of 19 (21%) Redblush grapefruit control trees became infected with CTV; seven of these Hamlin and all Redblush trees naturally infected with CTV also reacted with CTV-MCA13, indicating that they were infected with a severe isolate. Infection levels of CTV severe isolates in preinfected Hamlin treatments ranged from 0 to 50% based on CTV-MCA13 ELISA, with two treatments free of severe isolates. In Redblush grapefruit, infection levels of CTV severe isolates ranged from 0 to 22% but 11 CTV mild isolate treatments remained free from severe CTV infection. Decline symptoms were observed in 34 Hamlin and six grapefruit trees and supported the CTV-MCA13 ELISA results. Although substantial differences occurred between Hamlin treatments, results were n... Mostrar Tudo |
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Palavras-Chave: |
Aphid vectors; Mild strain cross protection; Mnoclonal antibody; Quick decline; tristeza control. |
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Thesagro: |
Elisa. |
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Thesaurus Nal: |
epidemiology. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02443naa a2200277 a 4500
001 1644223
005 1993-09-16
008 1991 bl uuuu u00u1 u #d
100 1 $aYOKOMI, R. K.
245 $aNatural spread of severe citrus tristeza virus isolates in citrus preinfected with mild CTV isolates.
260 $c1991
300 $ap.86-92
500 $aConference of the International Organization of Citrus virologists, 11., 1991, Riverside, California, Proceedings...
520 $aNatural spread of severe citrus tristeza virus (CTV) isolates was monitored in a 2-ha, 306-tree plot near Dundee, Florida. This plot, planted in spring 1986, consisted of Hamlin orange and Redblush grapefruit trees on sour orange rootstock preinfected with fourteen different CTV mild isolates. The trees were surveyed for symptoms of CTV decline in November 1988, March 1989, and October 1989, and assayed by enzyme-linked immunosorbent assay (ELISA). A monoclonal antibody, CTV-MCA13, which reacts specifically to severe decline-inducing isolates of CTV, and a broad-spectrum monoclonal antibody, 3DF1, were used in double sandwich indirect assay. After 3 yr of natural challenge, 3DF1-based ELISA indicated that 10 of 18 (56%) Hamlin and four of 19 (21%) Redblush grapefruit control trees became infected with CTV; seven of these Hamlin and all Redblush trees naturally infected with CTV also reacted with CTV-MCA13, indicating that they were infected with a severe isolate. Infection levels of CTV severe isolates in preinfected Hamlin treatments ranged from 0 to 50% based on CTV-MCA13 ELISA, with two treatments free of severe isolates. In Redblush grapefruit, infection levels of CTV severe isolates ranged from 0 to 22% but 11 CTV mild isolate treatments remained free from severe CTV infection. Decline symptoms were observed in 34 Hamlin and six grapefruit trees and supported the CTV-MCA13 ELISA results. Although substantial differences occurred between Hamlin treatments, results were not statistically different due to the ...
650 $aepidemiology
650 $aElisa
653 $aAphid vectors
653 $aMild strain cross protection
653 $aMnoclonal antibody
653 $aQuick decline
653 $atristeza control
700 1 $aGARNSEY, S. M.
700 1 $aPERMAR, T. A.
700 1 $aLEE, R. F.
700 1 $aYOUTSEY, C. O.
773 $tRiverside, California: International Organization of Citrus Virologists, 1991.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | AZEVEDO, F. A. de; MOURÃO FILHO, F. de A. A.; SCHINOR, E. H.; PAOLI, L. G. de; MENDES, B. M. J.; HARAKAVA, R.; GABRIEL, D. W.; LEE, R. F. GUS gene expression driven by a citrus promoter in transgenic tobacco and 'Valencia' sweet orange. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 41, n. 11, p. 1623-1628, nov. 2006 Título em português: Expressão do gene GUS controlado por promotor de citros em plantas transgênicas de tabaco e laranja 'Valência'.| Biblioteca(s): Embrapa Unidades Centrais. |
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