Data/hora: 01/06/2024 07:51:48

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 16/09/1993
Data da última atualização: | 16/09/1993
Autoria: | ROCHA-PENA, M. A.; LEE, R. F.; PERMAR, T. A.; YOKOMI, R. K.; GARNSEY, S. M.
Afiliação: | Univ. Florida, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL 33850, USA.
Título: | Use of enzyme-linked immunosorbent and dot-immunobinding assays to evaluate two mild-strain cross protection experiments after challenge with a severe citrus tristeza virus isolate.
Ano de publicação: | 1991
Fonte/Imprenta: | Riverside, California: International Organization of Citrus Virologists, 1991.
Páginas: | 93-102
Idioma: | Inglês
Notas: | Conference of the International Organization of Citrus Virologists, 11., 1991, Riverside, California, Proceedings...
Conteúdo: | In two experiments citrus trees on sour orange rootstock were pre-inoculated with 14 mild citrus tristeza virus (CTV) isolates and were then graft or aphid challenged with a severe quick decline CTV isolate after the trees were stablished in the field. Double antibody sandwich ELISA (DAS-ELISA) with polyclonal antibodies was used to detect CTV infection, and DAS-indirect ELISA using MCA-13 monoclonal antibody was used to detect severe CTV isolates. All trees in the two experiments were surveyed in August and November, 1989. DAS-ELISA in the August survey showed all the pre-inoculated plants to be infected with CTV, and only one plant in the aphid challenged experiment was positive when assayed with DAS-indirect ELISA using the MCA-13 monoclonal antibody. In N ovember, 1989, six addittional plants were positive when assayed by DAS-indirect ELISA with MCA-13. Some originally helthy uninoculated control plants were found to be infect by DAS-ELISA but not by DAS-indirect ELISA, indicating that some natural spread of mild CTV isolates had occured in both experiments. A dot-immunobinding assay was compared to DAS-ELISA and DAS-indirect ELISA and found to be more rapid and as sensitive in detection of CTV in crude preparations. The absence of reaction of the MCA-13 monoclonal antibody in both DAS-indirect ELISA and dot-immunobinding assay on most of the plants previously challenged with the severe CTV isolate suggests that the mild CTV isolates may by providing cross protection against the severe challenge isolates.
Palavras-Chave: | Citrus excelsa.
Thesagro: | Doença; Elisa; Pomelo.
Thesaurus Nal: | blight; Florida.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (13279-1) - ADC - PL - -- - 634.3040601 - C748p - 1992000183 - DIS