Data/hora: 01/06/2024 08:17:58

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 20/05/1993
Data da última atualização: | 20/05/1993
Autoria: | GARNSEY, S. M.; BAR-JOSEPH, M.; LEE, R. F.
Afiliação: | U.S. Department of Agriculture, Agricultural Research Service, Horticultural Research Laboratory, Orlando, FL 32803, USA.
Título: | Applications of serological indexing to develop control strategies for citrus tristeza virus.
Ano de publicação: | 1981
Fonte/Imprenta: | Tokyo, Japan: International Society of Citricultural, 1981.
Volume: | v.1
Páginas: | p.448-452
Idioma: | Inglês
Conteúdo: | The development of antisera specific to citrus tristeza virus (CTV) and use of CTV antisera with sensitive, rapid detection technique such as enzyme linked immunosorbent assay (ELISA) has greatly facilitated CTV indexing. These serological indexing techniques have created new opportunities to control CTV and new opportunities for research. In areas where CTV is not yet widespread, such as Israel, parts of the U.S., and parts of the Mediterranean Basin, serology has offered new hope for eradication or long-term suppression of CTV. The ELISA technique has been used to test more than 600,000 trees for CTV in a countrywide survey in Israel. New loci of infection, some symptomless, were located rapidly. Because results can be obtained within hours after sampling, diseased trees can be removed rapidly to reduce secondary spread. Serology has been used in Florida to test budwood trees for CTV and to monitor natural spread in field plantings. Serological indexing is adpted for the rapid, repeated testing of many samples in epidemiology studies, and detection of CTV in aphids by ELISA has been reported. ELISA is being used to identify CTV-resistant hybrids in crosses between Citrus and Poncirus trifoliata (L.) Raf. and other hybrids, and this rapid, quantitative measurrement of virus titer has improved screening. Serology is also being used in research on mild strain cross protection to verify presence of symptomless, mild isolates in inoculated, protected trees. However, mild and severe CTV strains...
Palavras-Chave: | Cross protection; Enzyme linked immunosorbent assay (ELISA); Host resistance; Immunodiffusion; Immunofluorescence; Radio immunosorbent assay; serologically specific electron microscopy (SSEM).
Thesaurus Nal: | epidemiology.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (13073-1) - ADD - PL - -- - 634.3040601 - I61p - 1992000203 - DIS