Data/hora: 22/06/2024 04:41:53

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 08/02/2021
Data da última atualização: | 09/02/2021
Autoria: | LEASTRO, M. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; NAVARRO, J. S.
Afiliação: | MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico, São Paulo; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, Universidad Politécnica de Valencia; JESÚSA. SÁNCHEZ NAVARRO, Universidad Politécnica de Valencia.
Título: | Unravelling the involvement of cilevirus p32 protein in the viral transport.
Ano de publicação: | 2021
Fonte/Imprenta: | Scientifc Reports, n.11, v.2943, 2021.
Idioma: | Inglês
Conteúdo: | Citrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model for the role of the p32 in the intra- and intercellular viral spread.
Thesagro: | Doença de Planta; Fruta Cítrica.
Thesaurus Nal: | Citrus; Plant diseases and disorders.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (33232-1) - UPC - AP - DD - Publicação digital -  -  - DIS