Data/hora: 21/06/2024 17:59:18

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	17/08/2021	
Data da última atualização: 	17/08/2021	
Autoria: 	LEAL, G. R.; GRACIOSA, M. A. G.; MONTEIRO, C. A. S.; PASOLINI, R.; CAMARGO, A. J. dos R.; OLIVEIRA, C. S.; VASCONCELOS, C. O. de P.; NOGUEIRA, L. A. G.; FERREIRA, A. M. R.; SERAPIAO, R. V.	
Afiliação: 	GABRIELA RAMOS LEAL, Universidade Federal Fluminense; MARIA ALICE GUIMARAES GRACIOSA, Pesagro; CLARA ANA SANTOS MONTEIRO, Universidade Federal Fluminense; RENATA PASOLINI, Pesagro; AGOSTINHO JORGE DOS REIS CAMARGO, Pesagro; CLARA SLADE OLIVEIRA, CNPGL; CARLOS OTAVIO DE PAULA VASCONCELOS, Universidade Federal Fluminense; LUIZ ALTAMIRO GARCIA NOGUEIRA, Universidade Federal Fluminense; ANA MARIA REIS FERREIRA, Universidade Federal Fluminense; RAQUEL VARELLA SERAPIAO, Pesagro.	
Título: 	The SPOM-adapted IVM system improves in vitro production of bovine embryos.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Theriogenology, v. 158, p. 277-282, 2020.	
DOI: 	https://doi.org/10.1016/j.theriogenology.2020.09.024	
Idioma: 	Inglês	
Conteúdo: 	This study aimed to test the effects of an IVM SPOM adaptation (SPOM-adapted IVM) on the production, total number of cells (TNC), apoptosis, and cryotolerance (post-warming survival and cytoskeleton actin integrity) of bovine IVP embryos. Two experiments were conducted with two experimental groups based on IVM treatment: A control group (TCM 199 without FCS) and an SPOM-adapted group (TCM 199 with forskolin and IBMX in pre-IVM and IVM with cilostamide). The first experiment evaluated embryo in vitro production, TNC, and apoptosis rate on D9 of development. In the second experiment, embryos were vitrified/warmed at D7 (control fresh and vitrified; SPOM-adapted fresh and vitrified) and assessed regarding post-warming survival rates and cytoskeleton actin integrity. Statistical analysis was performed using GraphPad INSTAT software at a significance level of 5%. An increase (p < 0.05) in blastocyst production was observed in the SPOM-adapted group comparing to the control group. There was no difference (p > 0.05) in the TNC or apoptosis rate between the groups. Regarding cryopreservation, no differences were found (p > 0.05) in actin integrity or post-warming survival rates between the vitrified groups. In both vitrified groups, we observed a significantly lower uninjured pattern of actin integrity compared to the fresh groups (p < 0.05). We conclude that the SPOM-adapted IVM system is beneficial for blastocyst production and does not affect the quality and cryotolerance of the produced embryos.	
Palavras-Chave: 	Cilostamida; Cilostamide.	
Thesagro: 	Bovino; Criopreservação; Embrião Animal; Reprodução Animal.	
Thesaurus Nal: 	Cryopreservation.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (25241-1) - UPC - AP - DD -  -  -  - DIS