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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
27/10/2015 |
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Data da última atualização: |
30/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; LEAL, C. L. V.; LIMA, M. R. de; CALLADO, M. del; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. |
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Afiliação: |
NAIARA ZOCCAL SARAIVA, CPATU; CLARA SLADE OLIVEIRA, CNPGL; Cláudia Lima Verde Leal, USP; Marina Ragagnin de Lima, UNESP; Maite Del Collado, UNESP / USP; Roberta Vantini, UNESP; Fabio Morato Monteiro, Centro APTA Bovinos de Corte, Instituto de Zootecnia; SIMONE CRISTINA MEO NICIURA, CPPSE; Joaquim Mansano Garcia, UNESP. |
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Título: |
Chemically induced enucleation of activated bovine oocytes: chromatin and microtubule organization and production of viable cytoplasts. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Zygote, v. 23, n. 6, p. 852-862, Dec. 2015. |
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DOI: |
http://dx.doi.org/10.1017/S0967199414000537 |
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Idioma: |
Inglês |
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Conteúdo: |
As the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6?70.0% and blastocyst yield of 15.5?24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos. MenosAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic deve... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Chemically induced enucleation; Microtubule; Nuclear transfer. |
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Thesaurus Nal: |
chromatin. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02641naa a2200289 a 4500
001 2027313
005 2022-05-30
008 2015 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1017/S0967199414000537$2DOI
100 1 $aSARAIVA, N. Z.
245 $aChemically induced enucleation of activated bovine oocytes$bchromatin and microtubule organization and production of viable cytoplasts.$h[electronic resource]
260 $c2015
520 $aAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6?70.0% and blastocyst yield of 15.5?24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos.
650 $achromatin
653 $aBovine
653 $aChemically induced enucleation
653 $aMicrotubule
653 $aNuclear transfer
700 1 $aOLIVEIRA, C. S.
700 1 $aLEAL, C. L. V.
700 1 $aLIMA, M. R. de
700 1 $aCALLADO, M. del
700 1 $aVANTINI, R.
700 1 $aMONTEIRO, F. M.
700 1 $aNICIURA, S. C. M.
700 1 $aGARCIA, J. M.
773 $tZygote$gv. 23, n. 6, p. 852-862, Dec. 2015.
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Embrapa Amazônia Oriental (CPATU) |
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| 41. |  | GIORDANO, L. B.; FERRAZ, E.; RESENDE, L. V.; ARAGÃO, F. A. S.; INOUE-NAGATA, A. K. Avaliação de linhagens de tomateiro para processamento industrial visando resistência a germinivírus. Horticultura Brasileira, Brasília, v. 21, n. 2, jul. 2003. Suplemento 2. Trabalho apresentado no 43º Congresso Brasileiro de Olericultura, 2003. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 21, n. 2, p. 350, jul. 2003. Suplemento 1.| Biblioteca(s): Embrapa Hortaliças. |
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| 42. | | SILVA, P. P.; INOUE-NAGATA, A. K.; RESENDE, R. O.; ÁVILA, A. C. Avaliação de patogenicidade de diferentes estirpes de Potato vírus Y (PVY) em batata. Tropical Plant Pathology, Brasília, DF, v. 33, p. S300, ago. 2008. Suplemento. Resumo VIR 060. Trabalho apresentado no 41. Congresso Brasileiro de Fitopatologia, 41. Annual Meeting of the Brazilian Phytopathological Society, Belo Horizonte, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 45. | | LUCINDA, N.; ROCHA, W. B. da; INOUE NAGATA, A. K.; NAGATA, T. Complete genome sequence of pepper yellow mosaic virus, a potyvirus, occurring in Brazil. Archives of Virology, New York, v. 157, n. 7, p. 1397-1401, Jul. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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| 46. | | LUCINDA, N.; INOUE-NAGATA, A. K.; KITAJIMA, E. W.; NAGATA, T. Complete genome sequencing of brugmansia suaveolens motlle virus. Virus Reviews & Research, São Paulo, v. 13, Supl. 2, p. 281, nov. 2008. Suplemento 2. Resumo. Trabalho apresentado no 19 National Meeting of Virology, 2008, Caxambu, Minas Gerais, Brasil.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Hortaliças. |
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| 47. | | LOVATO, F. A.; NAGATA, T.; ÁVILA, A. C. de; RESENDE, R. O.; INOUE-NAGATA, A. K. The complete nucleotide sequence of glycoprotein precursor of tomato chlorotic spot virus and groundnut ringspot virus. Fitopatologia Brasileira, Brasília, v. 29, p. S101-102, ago. 2004. Suplemento. Resumo . Trabalho apresentado no 37º Congresso Brasileiro de Fitopatologia, 2004.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 48. | | MARTINS, M. S. A.; HALWASS, M.; BEZERRA-AGASIE, I. C.; INOUE-NAGATA, A. K. Comparação de Tomato severe rugose virus isolado de tomate e pimenta. Tropical Plant Pathology, Brasília, DF, v. 33, p. S298, ago. 2008. Suplemento. Resumo VIR 053. Trabalho apresentado no 41. Congresso Brasileiro de Fitopatologia, 41. Annual Meeting of the Brazilian Phytopathological Society, Belo Horizonte, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 49. | | TEIXEIRA, E. C.; LUNA, C. P.; HALWASS, M.; INOUE-NAGATA, A. K. Caracterização molecular e biológica de um isolado de Sida micrantha mosaic virus que infecta Capsicum chinense. Tropical Plant Pathology, Brasília, DF, v. 33, p. S289, ago. 2008. Suplemento. Resumo VIR 017. Trabalho apresentado no 41. Congresso Brasileiro de Fitopatologia, 41. Annual Meeting of the Brazilian Phytopathological Society, Belo Horizonte, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 54. | | FERREIRA, G. B.; ÁVILA, A. C. de; NAGATA, T.; INOUE-NAGATA, A. K. Characterization of two potato virus y isolates collected in tomato and pepper plants. Virus Reviews & Research, Florianópolis, v. 9, p. 250-251, 2004. Suplement 1. Resumo. Trabalho apresentado no 15º National Meeting of Virology, São Pedro, 2004.| Biblioteca(s): Embrapa Hortaliças. |
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| 56. | | REGO, C. M.; NAKASU, E. Y. T.; INOUE-NAGATA, A. K. Begomovirus diversity in resistant and susceptible tomato plants. Virus Reviews and Research, Belo Horizonte, v. 20, p. 192, 2015. Supplement 1, ref. PIV 48. Edição dos Resumos do XXVI Brazilian Congress of Virology, X Mercour Meeting of Virology, 2015, Florianópolis.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 58. | | NAGATA, T.; INOUE-NAGATA, A.K.; DUSI, A. N.; KITAJIMA, E. W. Bidens mosaic potyvirus newly isolated from pea, its characteristics and serological relationship with other potyviruses. Fitopatologia Brasileira, Brasilia, v.20, n.3, p.473-478, set. 1995.| Biblioteca(s): Embrapa Hortaliças. |
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| 60. | | INOUE-NAGATA, A. K.; NAVAS-CASTILLO, J.; MELO, P. C. T. de; ÁVILA, A. C. de. Busca por Tomato yellow leaf curl virus e Tomato yellow leaf curl Sardinia virus em tomateiros. Horticultura Brasileira, Brasília, DF, v. 22, n. 4, p. 799-800, out./dez. 2004.| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 328 | |
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