| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Amazônia Oriental. |
|
Data corrente: |
27/10/2015 |
|
Data da última atualização: |
30/05/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SARAIVA, N. Z.; OLIVEIRA, C. S.; LEAL, C. L. V.; LIMA, M. R. de; CALLADO, M. del; VANTINI, R.; MONTEIRO, F. M.; NICIURA, S. C. M.; GARCIA, J. M. |
|
Afiliação: |
NAIARA ZOCCAL SARAIVA, CPATU; CLARA SLADE OLIVEIRA, CNPGL; Cláudia Lima Verde Leal, USP; Marina Ragagnin de Lima, UNESP; Maite Del Collado, UNESP / USP; Roberta Vantini, UNESP; Fabio Morato Monteiro, Centro APTA Bovinos de Corte, Instituto de Zootecnia; SIMONE CRISTINA MEO NICIURA, CPPSE; Joaquim Mansano Garcia, UNESP. |
|
Título: |
Chemically induced enucleation of activated bovine oocytes: chromatin and microtubule organization and production of viable cytoplasts. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
Zygote, v. 23, n. 6, p. 852-862, Dec. 2015. |
|
DOI: |
http://dx.doi.org/10.1017/S0967199414000537 |
|
Idioma: |
Inglês |
|
Conteúdo: |
As the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6?70.0% and blastocyst yield of 15.5?24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos. MenosAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic deve... Mostrar Tudo |
|
Palavras-Chave: |
Bovine; Chemically induced enucleation; Microtubule; Nuclear transfer. |
|
Thesaurus Nal: |
chromatin. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02641naa a2200289 a 4500
001 2027313
005 2022-05-30
008 2015 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1017/S0967199414000537$2DOI
100 1 $aSARAIVA, N. Z.
245 $aChemically induced enucleation of activated bovine oocytes$bchromatin and microtubule organization and production of viable cytoplasts.$h[electronic resource]
260 $c2015
520 $aAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6?70.0% and blastocyst yield of 15.5?24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos.
650 $achromatin
653 $aBovine
653 $aChemically induced enucleation
653 $aMicrotubule
653 $aNuclear transfer
700 1 $aOLIVEIRA, C. S.
700 1 $aLEAL, C. L. V.
700 1 $aLIMA, M. R. de
700 1 $aCALLADO, M. del
700 1 $aVANTINI, R.
700 1 $aMONTEIRO, F. M.
700 1 $aNICIURA, S. C. M.
700 1 $aGARCIA, J. M.
773 $tZygote$gv. 23, n. 6, p. 852-862, Dec. 2015.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Amazônia Oriental (CPATU) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Clima Temperado. |
|
Data corrente: |
26/10/2017 |
|
Data da última atualização: |
26/10/2017 |
|
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
|
Autoria: |
COMIN, J. J.; OLIVEIRA, R. A. de; LOSS, A.; SALUME, J. A.; SETE, P. B.; MÜLLER JUNIOR, V.; VENTURA, B. S.; SOUZA, M.; NAVA, G.; LOURENZI, C. R.; FERREIRA, P. A. A.; SOARES, C. R. F. S.; LOVATO, P. E.; MELO, G. W. B. de; BRUNETTO, G. |
|
Afiliação: |
Jucinei José Comin; Rodolfo Assis de Oliveira; Arcângelo Loss; Jamilli Almeida Salume; Paula Beatriz Sete; Vilmar Müller Júnior; Bárbara Santos Ventura; Monique Souza; GILBERTO NAVA, CPACT; Cledimar Rogério Lourenzi; Paulo Ademar Avelar Ferreira; Cláudio Roberto Fonseca Sousa Soares; Paulo Emílio Lovato; George Wellington Bastos de Melo; Gustavo Brunetto. |
|
Título: |
Uso de plantas de cobertura de inverno e ciclagem de nutrientes em pomares de pereira. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
In: CIOTTA, M.N.; BRUNETTO, G.; COMIN, J.; PASA, M.S.; SETE, P.B (orgs.). Workshop sobre frutificação e adubação de pereiras, 1.; Florianópolis, SC: Epagri, 2017. 120p. (Documentos 276) |
|
Páginas: |
p. 87-102 |
|
Idioma: |
Português |
|
Thesagro: |
Fruticultura; Pyrus Communis. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/165615/1/Gilberto-Nava-Anais-Workshop-Pereiras-18-08-2017.pdf
|
|
Marc: |
LEADER 01022naa a2200313 a 4500
001 2078260
005 2017-10-26
008 2017 bl uuuu u00u1 u #d
100 1 $aCOMIN, J. J.
245 $aUso de plantas de cobertura de inverno e ciclagem de nutrientes em pomares de pereira.$h[electronic resource]
260 $c2017
300 $ap. 87-102
650 $aFruticultura
650 $aPyrus Communis
700 1 $aOLIVEIRA, R. A. de
700 1 $aLOSS, A.
700 1 $aSALUME, J. A.
700 1 $aSETE, P. B.
700 1 $aMÜLLER JUNIOR, V.
700 1 $aVENTURA, B. S.
700 1 $aSOUZA, M.
700 1 $aNAVA, G.
700 1 $aLOURENZI, C. R.
700 1 $aFERREIRA, P. A. A.
700 1 $aSOARES, C. R. F. S.
700 1 $aLOVATO, P. E.
700 1 $aMELO, G. W. B. de
700 1 $aBRUNETTO, G.
773 $tIn: CIOTTA, M.N.; BRUNETTO, G.; COMIN, J.; PASA, M.S.; SETE, P.B (orgs.). Workshop sobre frutificação e adubação de pereiras, 1.; Florianópolis, SC: Epagri, 2017. 120p. (Documentos 276)
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Clima Temperado (CPACT) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
