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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
20/12/2019 |
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Data da última atualização: |
20/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
IARK, D.; BUZZO, A. J. dos R.; GARCIA, J. A. A.; CORREA, V. G.; HELM, C. V.; CORRÊA, R. C. G.; PERALTA, R. A.; MOREIRA, R. de F. P. M.; BRACHT, A.; PERALTA, R. M. |
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Afiliação: |
Daiane Iark, UEM; Ana Júlia dos Reis Buzzo, UEM; Jéssica Amanda Andrade Garcia, UEM; Vanesa Gesser Correa, UEM; CRISTIANE VIEIRA HELM, CNPF; Rúbia Carvalho Gomes Corrêa, UEM; Rosely A. Peralta, UFSC; Regina de Fátima Peralta Muniz Moreira, UFSC; Adelar Bracht, UEM; Rosane Marina Peralta, UEM. |
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Título: |
Enzymatic degradation and detoxification of azo dye Congo red by a new laccase from Oudemansiella canarii. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Bioresource Technology, 289, article 121655, Oct. 2019. 7 p. |
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DOI: |
10.1016/j.biortech.2019.121655 |
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Idioma: |
Inglês |
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Conteúdo: |
A single laccase with molecular weight of 41 kDa was produced by the white-rot fungus Oudemansiella canarii cultured on solid state fermentation using a mixture of sugarcane bagasse-wheat bran as substrate. The enzyme (5 U) was able to decolourize 80% of 50 mg/L Congo red within 24 h at 30 °C and pH 5.5. The relationship between the decolorization rate and dye concentration obeyed Michaelis?Menten kinetics, with KM and Vmax values of 46.180 ± 6.245 µM and 1.840 ± 0.101 µmol/min, respectively. Fourier transform infrared spectroscopy (FTIR) and mass spectrometry allowed to conclude that the laccase acts not only on the dye chromophore group, but also that it cleaves different covalent bonds, causing an effective fragmentation of the molecule. The action of the laccase caused a significant reduction in toxicity, as indicated by the Microtox test. In conclusion, O. canarii laccase could be useful in future biological strategies aiming at degrading azo dyes. |
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Palavras-Chave: |
Biorremediação; Dye degradation; Lacase. |
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Thesaurus NAL: |
Azo dyes; Laccase; White-rot fungi. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01891naa a2200313 a 4500
001 2117496
005 2019-12-20
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1016/j.biortech.2019.121655$2DOI
100 1 $aIARK, D.
245 $aEnzymatic degradation and detoxification of azo dye Congo red by a new laccase from Oudemansiella canarii.$h[electronic resource]
260 $c2019
520 $aA single laccase with molecular weight of 41 kDa was produced by the white-rot fungus Oudemansiella canarii cultured on solid state fermentation using a mixture of sugarcane bagasse-wheat bran as substrate. The enzyme (5 U) was able to decolourize 80% of 50 mg/L Congo red within 24 h at 30 °C and pH 5.5. The relationship between the decolorization rate and dye concentration obeyed Michaelis?Menten kinetics, with KM and Vmax values of 46.180 ± 6.245 µM and 1.840 ± 0.101 µmol/min, respectively. Fourier transform infrared spectroscopy (FTIR) and mass spectrometry allowed to conclude that the laccase acts not only on the dye chromophore group, but also that it cleaves different covalent bonds, causing an effective fragmentation of the molecule. The action of the laccase caused a significant reduction in toxicity, as indicated by the Microtox test. In conclusion, O. canarii laccase could be useful in future biological strategies aiming at degrading azo dyes.
650 $aAzo dyes
650 $aLaccase
650 $aWhite-rot fungi
653 $aBiorremediação
653 $aDye degradation
653 $aLacase
700 1 $aBUZZO, A. J. dos R.
700 1 $aGARCIA, J. A. A.
700 1 $aCORREA, V. G.
700 1 $aHELM, C. V.
700 1 $aCORRÊA, R. C. G.
700 1 $aPERALTA, R. A.
700 1 $aMOREIRA, R. de F. P. M.
700 1 $aBRACHT, A.
700 1 $aPERALTA, R. M.
773 $tBioresource Technology, 289, article 121655, Oct. 2019. 7 p.
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