| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
28/05/1996 |
|
Data da última atualização: |
28/05/1996 |
|
Autoria: |
MOYA-LEON, M.A.; JOHN, P. |
|
Afiliação: |
Department of Agricultural Botany, Plant science laboratories, The University of Reading, Reading RG6 6AS, U.K. |
|
Título: |
Purification and biochemical characterization of 1-Aminocyclopropane-1-carboxylate oxidase from banana fruit. |
|
Ano de publicação: |
1995 |
|
Fonte/Imprenta: |
Phytochemistry, v.39, n.1, p.15-20, 1995. |
|
Idioma: |
Inglês |
|
Conteúdo: |
A novel method using PEG and ammonium sulphate was used to concentrate ACC oxidase from a crude extract prepared fron the pulp of ripe fruit of banana (Musa AAA group, Cavendish subgroup). The 145-fold purification of the ACC oxidase to electrophoretic homogeneity was carried out in four chromatographic steps: hydrophobic interaction, anion exchange, chromatofocusing and gel filtration. The molecular mass of the purified enzyme was estimated to be 40 000 by gel filtration and 36 000 by SDS-PAGE, indicating that the enzyme is active asa monomer. The enzyme was recognized by a polyclonal antibody directed against a recombinant polypeptide derived from the tomato ACC oxidase, showing that the banana enzyme shares immunogenic epitopes with ACC oxidasses from other fruits. An isoelectric point at pH 4.9 was estimated by chromatofocusing. The Km with respct to ACC was found to be 56 uM. Ascorbate, fe and sodium bicarbonate were required for the activity of the enzyme, and the presence of ascorbate was found to be necessary to avoid loss of activity during the purification. In general, the properties of the ACC oxidasse from this monocotyledonous source resemble those of the enzyme previously purified from apple fruit. |
|
Palavras-Chave: |
1-aminocylopropane-1-carboxylate (ACC) oxidase; Cavendish subgroup; Cultivar cavendish; Enzimas; Enzime; Enzimology; Fruit; Hormone; Musa AAA group; Musa spp. |
|
Thesagro: |
Banana; Fruta; Hormônio; Musaceae. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02038naa a2200301 a 4500
001 1634334
005 1996-05-28
008 1995 bl uuuu u00u1 u #d
100 1 $aMOYA-LEON, M.A.
245 $aPurification and biochemical characterization of 1-Aminocyclopropane-1-carboxylate oxidase from banana fruit.
260 $c1995
520 $aA novel method using PEG and ammonium sulphate was used to concentrate ACC oxidase from a crude extract prepared fron the pulp of ripe fruit of banana (Musa AAA group, Cavendish subgroup). The 145-fold purification of the ACC oxidase to electrophoretic homogeneity was carried out in four chromatographic steps: hydrophobic interaction, anion exchange, chromatofocusing and gel filtration. The molecular mass of the purified enzyme was estimated to be 40 000 by gel filtration and 36 000 by SDS-PAGE, indicating that the enzyme is active asa monomer. The enzyme was recognized by a polyclonal antibody directed against a recombinant polypeptide derived from the tomato ACC oxidase, showing that the banana enzyme shares immunogenic epitopes with ACC oxidasses from other fruits. An isoelectric point at pH 4.9 was estimated by chromatofocusing. The Km with respct to ACC was found to be 56 uM. Ascorbate, fe and sodium bicarbonate were required for the activity of the enzyme, and the presence of ascorbate was found to be necessary to avoid loss of activity during the purification. In general, the properties of the ACC oxidasse from this monocotyledonous source resemble those of the enzyme previously purified from apple fruit.
650 $aBanana
650 $aFruta
650 $aHormônio
650 $aMusaceae
653 $a1-aminocylopropane-1-carboxylate (ACC) oxidase
653 $aCavendish subgroup
653 $aCultivar cavendish
653 $aEnzimas
653 $aEnzime
653 $aEnzimology
653 $aFruit
653 $aHormone
653 $aMusa AAA group
653 $aMusa spp
700 1 $aJOHN, P.
773 $tPhytochemistry$gv.39, n.1, p.15-20, 1995.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 2 | |
| 2. |  | DIAZ, V. S.; ZANATTA, R. A. F.; AMÊNDOLA, D.; PAOLINETI, V. W.; LIMA, A. D.; SEOANE, C. E. S. Produção de açaí a partir dos frutos do palmito juçara. In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 7., 2008, Colombo. Anais. Colombo: Embrapa Florestas, 2008. 1 CD-ROM. Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
|    |
| Registros recuperados : 2 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
