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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
22/03/2012 |
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Data da última atualização: |
22/03/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ELLINGSON, J. S.; WANG, Y.; LAYTON, S.; ZANELLA, J. R. C.; ROOF, M. B.; FAABERG, K. S. |
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Afiliação: |
JOSHUA S. ELLINGSON, Iowa State University; YUE WANG, University of Minnesota; SARAH LAYTON, Boerhinger Ingelheim Vetmedica; JANICE REIS CIACCI ZANELLA, CNPSA; MICHAEL B. ROOF, Boehringer Ingelheim Vetmedica; KAY S. FAABERG, University of Minnesota. |
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Título: |
Vaccine efficacy of porcine reproductive and respiratory syndrome virus chimeras. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Vaccine, v. 28, n. 14, p. 2679-2686, 2010. |
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Idioma: |
Inglês |
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Notas: |
Projeto/Plano de Ação: 03.09.00.046. |
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Conteúdo: |
The vaccine efficacy of six PRRSV Type 2 infectious clones, including five chimeras and a strain-specific deletion mutant, were examined using a respiratory challenge model in growing swine. The chimeras were constructed from different combinations of a licensed modified live vaccine (Ingelvac® PRRS MLV) and a virulent field isola e (wt MN184) which differ by 14.3% on a nucleotide basis, while the deletion mutant tested had a broad deletion in the nsp2 region of strain MN184. The appearance of antibodies and virus characterization revealed regions of the genome that could influence PRRSV replication in vivo. Swine growth, clinical signs and lung lesions were also monitored. Average daily weight gain was negatively and directly impacted by some vaccines, and after challenge, vaccination with different constructs led to variable weight gain. We determined that 3 of the tested chimeras, including two previously published chimeras [1] and one in which strain MN184 ORF5-6 was placed on the background of Ingelvac® PRRS MLV were able to prevent lung consolidation to a similar extent as traditionally prepared cell-passaged attenuated vaccines. The study suggested that only specific chimeras can attenuate clinical signs in swine and that attenuation cannot be directly linked to primary virus replication. Additionally, the strain MN184 deletion mutant was not found to have been sufficiently attenuated nor efficacious against heterologous challenge with strain JA-142. |
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Thesagro: |
Suíno; Vacina; Vírus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02126naa a2200229 a 4500
001 1919883
005 2012-03-22
008 2011 bl uuuu u00u1 u #d
100 1 $aELLINGSON, J. S.
245 $aVaccine efficacy of porcine reproductive and respiratory syndrome virus chimeras.$h[electronic resource]
260 $c2011
500 $aProjeto/Plano de Ação: 03.09.00.046.
520 $aThe vaccine efficacy of six PRRSV Type 2 infectious clones, including five chimeras and a strain-specific deletion mutant, were examined using a respiratory challenge model in growing swine. The chimeras were constructed from different combinations of a licensed modified live vaccine (Ingelvac® PRRS MLV) and a virulent field isola e (wt MN184) which differ by 14.3% on a nucleotide basis, while the deletion mutant tested had a broad deletion in the nsp2 region of strain MN184. The appearance of antibodies and virus characterization revealed regions of the genome that could influence PRRSV replication in vivo. Swine growth, clinical signs and lung lesions were also monitored. Average daily weight gain was negatively and directly impacted by some vaccines, and after challenge, vaccination with different constructs led to variable weight gain. We determined that 3 of the tested chimeras, including two previously published chimeras [1] and one in which strain MN184 ORF5-6 was placed on the background of Ingelvac® PRRS MLV were able to prevent lung consolidation to a similar extent as traditionally prepared cell-passaged attenuated vaccines. The study suggested that only specific chimeras can attenuate clinical signs in swine and that attenuation cannot be directly linked to primary virus replication. Additionally, the strain MN184 deletion mutant was not found to have been sufficiently attenuated nor efficacious against heterologous challenge with strain JA-142.
650 $aSuíno
650 $aVacina
650 $aVírus
700 1 $aWANG, Y.
700 1 $aLAYTON, S.
700 1 $aZANELLA, J. R. C.
700 1 $aROOF, M. B.
700 1 $aFAABERG, K. S.
773 $tVaccine$gv. 28, n. 14, p. 2679-2686, 2010.
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| 1. |  | ELLINGSON, J. S.; WANG, Y.; LAYTON, S.; ZANELLA, J. R. C.; ROOF, M. B.; FAABERG, K. S. Vaccine efficacy of porcine reproductive and respiratory syndrome virus chimeras. Vaccine, v. 28, n. 14, p. 2679-2686, 2010. Projeto/Plano de Ação: 03.09.00.046.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
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