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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
05/06/2018 |
Data da última atualização: |
30/11/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ABREU, F. R. M.; DEDICOVA, B.; VIANELLO, R. P.; LANNA, A. C.; OLIVEIRA, J. A. V. de; VIEIRA, A. F.; MORAIS, O. P.; MENDONÇA, J. A.; BRONDANI, C. |
Afiliação: |
FERNANDA RAQUEL MARTINS ABREU, UFG; BEATA DEDICOVA, CIAT; ROSANA PEREIRA VIANELLO, CNPAF; ANNA CRISTINA LANNA, CNPAF; JOAO AUGUSTO VIEIRA DE OLIVEIRA, UFG; ARIADNA FARIA VIEIRA, UFG; ODILON PEIXOTO MORAIS; JOAO ANTONIO MENDONCA, CNPAF; CLAUDIO BRONDANI, CNPAF. |
Título: |
Overexpression of a phospholipase (OsPLDa1) for drought tolerance in upland rice (Oryza sativa L.). |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Protoplasma, v. 255, n. 6, p. 1751-1761, Nov. 2018. |
ISSN: |
1615-6102 |
DOI: |
10.1007/s00709-018-1265-6 |
Idioma: |
Inglês |
Conteúdo: |
This work aimed to evaluate the drought tolerance of transformed plants of the cultivar BRSMG Curinga that overexpress the rice phospholipase D &1(OsPLD&1) gene. The productivity of independent transformation event plants of the OsPLD&1 gene was evaluated in an experiment where 19 days of water deficit were applied at the reproductive stage, a very strict growing condition for upland rice. The non-genetically modified cultivar (NGM) under drought treatment reduced productivity by 89% compared with that under irrigated treatment, whereas transformed plants (PLD&1_E2) reduced productivity by only 41%. After the drought treatment, the PLD&1_E2 plants productivity was five times greater than that of the NGM plant. Moreover, no adverse effects on growth and development of the transgenic plants were observed. Seven days after the resumption of irrigation, PLD&1_E2 plants had higher stomatal conductance, greater photosynthetic rate, and transpiration rate than did NGM plants, as well as a higher expression level of the OsPLD&1 gene. A delay in the senescence process was observed in these PLD&1_E2 plants, and this was determined for the recovery of photosynthesis, with greater expression of the Rubisco and lower expression of the SOD. This finding was suggestive of decreased oxidative stress, probably due to gas exchange by the partial closure of the stomata of these transformed plants, which prevented the formation of reactive oxygen species. OsPLD&1 gene overexpression resulted in a reduction in production loss under severe water deficit and revealed a possibility for the development of upland rice cultivars that are more tolerant to extreme drought conditions. MenosThis work aimed to evaluate the drought tolerance of transformed plants of the cultivar BRSMG Curinga that overexpress the rice phospholipase D &1(OsPLD&1) gene. The productivity of independent transformation event plants of the OsPLD&1 gene was evaluated in an experiment where 19 days of water deficit were applied at the reproductive stage, a very strict growing condition for upland rice. The non-genetically modified cultivar (NGM) under drought treatment reduced productivity by 89% compared with that under irrigated treatment, whereas transformed plants (PLD&1_E2) reduced productivity by only 41%. After the drought treatment, the PLD&1_E2 plants productivity was five times greater than that of the NGM plant. Moreover, no adverse effects on growth and development of the transgenic plants were observed. Seven days after the resumption of irrigation, PLD&1_E2 plants had higher stomatal conductance, greater photosynthetic rate, and transpiration rate than did NGM plants, as well as a higher expression level of the OsPLD&1 gene. A delay in the senescence process was observed in these PLD&1_E2 plants, and this was determined for the recovery of photosynthesis, with greater expression of the Rubisco and lower expression of the SOD. This finding was suggestive of decreased oxidative stress, probably due to gas exchange by the partial closure of the stomata of these transformed plants, which prevented the formation of reactive oxygen species. OsPLD&1 gene overexpression resulted in... Mostrar Tudo |
Thesagro: |
Arroz; Engenharia Genética; Oryza Sativa; Resistência a Seca; Stress. |
Thesaurus Nal: |
Abiotic stress; Drought tolerance; Gene expression; Genetic engineering; Phospholipases; Rice. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02737naa a2200373 a 4500 001 2092221 005 2018-11-30 008 2018 bl uuuu u00u1 u #d 022 $a1615-6102 024 7 $a10.1007/s00709-018-1265-6$2DOI 100 1 $aABREU, F. R. M. 245 $aOverexpression of a phospholipase (OsPLDa1) for drought tolerance in upland rice (Oryza sativa L.).$h[electronic resource] 260 $c2018 520 $aThis work aimed to evaluate the drought tolerance of transformed plants of the cultivar BRSMG Curinga that overexpress the rice phospholipase D &1(OsPLD&1) gene. The productivity of independent transformation event plants of the OsPLD&1 gene was evaluated in an experiment where 19 days of water deficit were applied at the reproductive stage, a very strict growing condition for upland rice. The non-genetically modified cultivar (NGM) under drought treatment reduced productivity by 89% compared with that under irrigated treatment, whereas transformed plants (PLD&1_E2) reduced productivity by only 41%. After the drought treatment, the PLD&1_E2 plants productivity was five times greater than that of the NGM plant. Moreover, no adverse effects on growth and development of the transgenic plants were observed. Seven days after the resumption of irrigation, PLD&1_E2 plants had higher stomatal conductance, greater photosynthetic rate, and transpiration rate than did NGM plants, as well as a higher expression level of the OsPLD&1 gene. A delay in the senescence process was observed in these PLD&1_E2 plants, and this was determined for the recovery of photosynthesis, with greater expression of the Rubisco and lower expression of the SOD. This finding was suggestive of decreased oxidative stress, probably due to gas exchange by the partial closure of the stomata of these transformed plants, which prevented the formation of reactive oxygen species. OsPLD&1 gene overexpression resulted in a reduction in production loss under severe water deficit and revealed a possibility for the development of upland rice cultivars that are more tolerant to extreme drought conditions. 650 $aAbiotic stress 650 $aDrought tolerance 650 $aGene expression 650 $aGenetic engineering 650 $aPhospholipases 650 $aRice 650 $aArroz 650 $aEngenharia Genética 650 $aOryza Sativa 650 $aResistência a Seca 650 $aStress 700 1 $aDEDICOVA, B. 700 1 $aVIANELLO, R. P. 700 1 $aLANNA, A. C. 700 1 $aOLIVEIRA, J. A. V. de 700 1 $aVIEIRA, A. F. 700 1 $aMORAIS, O. P. 700 1 $aMENDONÇA, J. A. 700 1 $aBRONDANI, C. 773 $tProtoplasma$gv. 255, n. 6, p. 1751-1761, Nov. 2018.
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Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca(s): |
Embrapa Roraima. |
Data corrente: |
08/02/2008 |
Data da última atualização: |
17/07/2017 |
Tipo da produção científica: |
Folder/Folheto/Cartilha |
Autoria: |
FERREIRA, G. B.; SMIDERLE, O. J.; NOBREGA, M. B. de M.; GONDIM, T. M. de S.; ANDRADE, F. P. de; SUASSUNA, N. D.; COUTINHO, W. de M.; SEVERINO, L. S.; FREIRE, R. M. M.; VASCONCELOS, R. A.; SILVA, G. A. da; MOTA, J. R.; LEITE, M. do C. de L.; ARAGÃO, W. M. de; CARVALHO, H. W. L. de; OLIVEIRA, I. R. de; LIRA, M. A.; SANTOS, F. das C.; SANTIAGO, A. N.; DOURADO, V. V.; MILANI, M. |
Afiliação: |
Gilvan Barbosa Ferreira, CPAF-RR; Oscar José Smirdele, CPAF-RR; Maíra Milani, Embrapa Algodão; Márcia Barreto de Medeiros Nobrega, Embrapa Algodão; Tarcísio Marcos de Sousa Gondim, Embrapa Algodão; Francisco Pereira de Andrade, Embrapa Algodão; Nelson Di. |
Título: |
BRS Energia |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
Campina Grande: Embrapa Algodão, 2007. |
Páginas: |
não paginado |
Idioma: |
Português |
Notas: |
Folder |
Palavras-Chave: |
Altura da BRS energia; BRS Energia; Folhas; Número de frutos; Sementes; Tamanho do cacho; Teor de óleo. |
Thesagro: |
Caule. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAF-RR/7099/1/Folder_brs_energia.pdf
|
Marc: |
LEADER 01186nam a2200457 a 4500 001 1691482 005 2017-07-17 008 2007 bl uuuu u0uu1 u #d 100 1 $aFERREIRA, G. B. 245 $aBRS Energia 260 $aCampina Grande: Embrapa Algodão$c2007 300 $anão paginado 500 $aFolder 650 $aCaule 653 $aAltura da BRS energia 653 $aBRS Energia 653 $aFolhas 653 $aNúmero de frutos 653 $aSementes 653 $aTamanho do cacho 653 $aTeor de óleo 700 1 $aSMIDERLE, O. J. 700 1 $aNOBREGA, M. B. de M. 700 1 $aGONDIM, T. M. de S. 700 1 $aANDRADE, F. P. de 700 1 $aSUASSUNA, N. D. 700 1 $aCOUTINHO, W. de M. 700 1 $aSEVERINO, L. S. 700 1 $aFREIRE, R. M. M. 700 1 $aVASCONCELOS, R. A. 700 1 $aSILVA, G. A. da 700 1 $aMOTA, J. R. 700 1 $aLEITE, M. do C. de L. 700 1 $aARAGÃO, W. M. de 700 1 $aCARVALHO, H. W. L. de 700 1 $aOLIVEIRA, I. R. de 700 1 $aLIRA, M. A. 700 1 $aSANTOS, F. das C. 700 1 $aSANTIAGO, A. N. 700 1 $aDOURADO, V. V. 700 1 $aMILANI, M.
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