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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
04/10/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA, I.; ANTUNES, G. R.; PAIVA, D. de S.; LANGE, C. C.; GUIMARÃES, S. E. F.; MARTINS, M. F. |
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Afiliação: |
ISABELA FONSECA, UFV; GUSTAVO RESENDE ANTUNES, UFJF; DAISYLÉA DE SOUZA PAIVA, UFJF; CARLA CHRISTINE LANGE, CNPGL; SIMONE ELISA FACIONI GUIMARÃES, UFV; MARTA FONSECA MARTINS, CNPGL. |
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Título: |
Differential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 10, n. 3, p. 1295-1303, 2011. |
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DOI: |
https://doi.org/10.4238/vol10-3gmr1096 |
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Idioma: |
Inglês |
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Conteúdo: |
Among the potential public health problems of animal production, infectious-contagious diseases stand out. Mastitis is among the main diseases affecting dairy cattle. One of the most promising options to reduce the problems caused by this disease, besides proper sanitary and management practices, is selective breeding of resistant animals. To shed light on the immune response mechanisms involved in the resistance/susceptibility phenotype to this disease, we quantified the relative expression of the genes IL-2, IL-6, IL-8, IL-12, IFN-γ, TNF-α, TLR-2, SEMA5A, and FEZL in cells of crossbreed dairy cows, divided into two groups, one healthy and the other suffering from clinical mastitis. Total RNA was extracted from the cells in the milk from the animals in each group (with and without clinical mastitis). Gene expression was determined using the real-time PCR method. The levels of gene expression were compared, and the cows with mastitis were found to express 2.5 times more TLR-2 than those free of mastitis (P < 0.05). There were no significant differences in the expression of the other genes. |
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Palavras-Chave: |
Real-time PCR; Resistance to mastitis; Toll-like receptor 2 gene. |
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Thesaurus Nal: |
immune response. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/54216/1/Differential-expression-of-genes-during.pdf
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Marc: |
LEADER 01871naa a2200241 a 4500
001 1902309
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4238/vol10-3gmr1096$2DOI
100 1 $aFONSECA, I.
245 $aDifferential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows.$h[electronic resource]
260 $c2011
520 $aAmong the potential public health problems of animal production, infectious-contagious diseases stand out. Mastitis is among the main diseases affecting dairy cattle. One of the most promising options to reduce the problems caused by this disease, besides proper sanitary and management practices, is selective breeding of resistant animals. To shed light on the immune response mechanisms involved in the resistance/susceptibility phenotype to this disease, we quantified the relative expression of the genes IL-2, IL-6, IL-8, IL-12, IFN-γ, TNF-α, TLR-2, SEMA5A, and FEZL in cells of crossbreed dairy cows, divided into two groups, one healthy and the other suffering from clinical mastitis. Total RNA was extracted from the cells in the milk from the animals in each group (with and without clinical mastitis). Gene expression was determined using the real-time PCR method. The levels of gene expression were compared, and the cows with mastitis were found to express 2.5 times more TLR-2 than those free of mastitis (P < 0.05). There were no significant differences in the expression of the other genes.
650 $aimmune response
653 $aReal-time PCR
653 $aResistance to mastitis
653 $aToll-like receptor 2 gene
700 1 $aANTUNES, G. R.
700 1 $aPAIVA, D. de S.
700 1 $aLANGE, C. C.
700 1 $aGUIMARÃES, S. E. F.
700 1 $aMARTINS, M. F.
773 $tGenetics and Molecular Research$gv. 10, n. 3, p. 1295-1303, 2011.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 12 | |
| 2. |  | OSTER, A. H.; MORAES, M. G. de; VALDEBENITO-SANHUEZA, R. M.; CORRENT, A.; BENDER, R. J. Isolamento de RNA para o estudo da expressão diferencial de genes durante a interação entre frutos de maçãs e Botryosphaeria dothidea. In: CONGRESSO BRASILEIRO DE FRUTICULTURA,18., 2004, Florianópolis. Tecnologia, competitividade, sustentabilidade: anais... Florianópolis: SBF, 2004. Não paginado. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 8. | | ALVES, R. M. M.; LEMOS, G. B. de; COSTA, M. P.; BARDUCHE, D. MORAES, M. G. de; CASTRO, E. M. de; C. FILHA, A. C. Efeitos do thidiazuron na atividade da fosfatase ácida durante a micropropagação da arnica (Solidago microglosa). In: CONGRESSO BRASILEIRO DE FISIOLOGIA VEGETAL, 5., 1995, Lavras. Resumos... Lavras: UFLA, 1995. p. 196.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amapá. |
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| 9. | | OLIVEIRA, E. G. de; SANTOS, F. J. de S.; OLIVEIRA, V. Q. de; MESQUISTA, P. E. C.; MORAES, M. G. de; SOUSA, R. R. de; GOMES, I. G. R. F. Influence of stocking density on water quality and growth performance in production of juvenile pirarucu, Arapaima gigas, in irrigation canals. Brazilian Journal of Development, Curitiba, v. 6, n. 2, p. 8725-8743, Feb. 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 10. | | OLIVEIRA, E. G. de; PINHEIRO, A. B.; OLIVEIRA, V. Q. de; SILVA JÚNIOR, A. R. M. da; MORAES, M. G. de; ROCHA, I. R. C. B.; SOUSA, R. R. de; COSTA, F. H. F. Effects of stocking density on the performance of juvenile pirarucu (Arapaima gigas) in cages. Aquaculture, Amsterdam, v. 370-371, p. 96-101, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 11. | | OLIVEIRA, V. Q. de; MATOS, A. R. B.; BEZERRA, T. A.; MESQUITA, P. E. C.; OLIVEIRA, V. Q. de; MORAES, A. M. de; OLIVEIRA, E. G. de; MORAES, M. G. de; SOUSA, R. R. de; COSTA, F. H. F. Preliminary studies on the optimum feeding rate for pirarucu Arapaima gigas juveniles reared in floating cages. International Journal of Aquaculture, v. 3, n. 25, p. 147-151, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 12. | | COSTA, N. B.; FARIA, D. R.; MENDONÇA, S. M.; MORAES, M. G. de; COELHO, G. R. C.; FILIPPI, M. C. C. de; BHOSALE, R.; CASTRO, A. P. de; LANNA, A. C. Silicon and bioagents pretreatments synergistically improve upland rice performance during water stress. Plant Stress, v. 7, 100142, Mar. 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 12 | |
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