| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Milho e Sorgo. |
|
Data corrente: |
29/10/2012 |
|
Data da última atualização: |
18/05/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
LANA, U. G. de P.; GOMES, E. A.; SILVA, D. D. da; COSTA, R. V. da; COTA, L. V.; PARREIRA, D. F.; SOUZA, I. R. P.; GUIMARAES, C. T. |
|
Afiliação: |
UBIRACI GOMES DE PAULA LANA, CNPMS; ELIANE APARECIDA GOMES, CNPMS; DAGMA DIONISIA DA SILVA, CNPMS; RODRIGO VERAS DA COSTA, CNPMS; LUCIANO VIANA COTA, CNPMS; ISABEL REGINA PRAZERES DE SOUZA, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS. |
|
Título: |
Detection and molecular diversity of Pantoea ananatis associated with white spot disease in maize, sorghum and crabgrass in Brazil. |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
Journal of Phytopathology, Berlin, v. 160, p. 441-448, 2012. |
|
DOI: |
10.1111/j.1439-0434.2012.01924.x |
|
Idioma: |
Inglês |
|
Conteúdo: |
Bacteria of the genus Pantoea have become important plant pathogens worldwide in recent years. Pantoea ananatis was reported as the cause of maize white spot, a serious maize disease in Brazil, causing significant yield losses. However, very little information is available about how to detect this pathogen, its genetic variability and the putative alternative hosts in maize-growing areas. To address these issues, we implemented a rapid and efficient PCR-based method to identify P. ananatis isolated from leaves showing white spot symptoms and evaluated its genetic diversity in maize, sorghum and crabgrass. Of the 29 bacteria isolated from typical water-soaked lesions of white spot disease that produced yellow colonies, 15 isolates were identified as P. ananatis by 16S rDNA sequencing and correctly detected by the PCR reaction, amplifying a specific fragment of the ice nucleation gene (ina). These P. ananatis isolates included 13 from maize, one from sorghum and one from crabgrass, while the other 14 yellow colony isolates were from other bacterial species, including two Pantoea species (Pantoea dispersa and Pantoea agglomerans) that were not amplified by the ina primers. These results indicate that the optimized PCR assay can be used to detect P. ananatis isolated from white spot lesions and could be used as a large-scale and cost-effective method of detecting this pathogen in leaf lesions on maize and other grasses. All isolates were evaluated for hypersensitive response (HR) on tobacco, revealing that some P. ananatis were able to induce HR. The high genetic variability revealed by rep-PCR did not differentiated the P. ananatis isolates based on their hosts or HR reaction. The detection, characterization and diversity of P.ananatis from maize, sorghum and crabgrass in our study can be applied in understanding epidemiology and designing control strategies for maize white spot disease in Brazil. MenosBacteria of the genus Pantoea have become important plant pathogens worldwide in recent years. Pantoea ananatis was reported as the cause of maize white spot, a serious maize disease in Brazil, causing significant yield losses. However, very little information is available about how to detect this pathogen, its genetic variability and the putative alternative hosts in maize-growing areas. To address these issues, we implemented a rapid and efficient PCR-based method to identify P. ananatis isolated from leaves showing white spot symptoms and evaluated its genetic diversity in maize, sorghum and crabgrass. Of the 29 bacteria isolated from typical water-soaked lesions of white spot disease that produced yellow colonies, 15 isolates were identified as P. ananatis by 16S rDNA sequencing and correctly detected by the PCR reaction, amplifying a specific fragment of the ice nucleation gene (ina). These P. ananatis isolates included 13 from maize, one from sorghum and one from crabgrass, while the other 14 yellow colony isolates were from other bacterial species, including two Pantoea species (Pantoea dispersa and Pantoea agglomerans) that were not amplified by the ina primers. These results indicate that the optimized PCR assay can be used to detect P. ananatis isolated from white spot lesions and could be used as a large-scale and cost-effective method of detecting this pathogen in leaf lesions on maize and other grasses. All isolates were evaluated for hypersensitive response (HR... Mostrar Tudo |
|
Palavras-Chave: |
Diversidade genética. |
|
Thesagro: |
Doença de planta; Mancha branca. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02743naa a2200253 a 4500
001 1938414
005 2017-05-18
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1111/j.1439-0434.2012.01924.x$2DOI
100 1 $aLANA, U. G. de P.
245 $aDetection and molecular diversity of Pantoea ananatis associated with white spot disease in maize, sorghum and crabgrass in Brazil.$h[electronic resource]
260 $c2012
520 $aBacteria of the genus Pantoea have become important plant pathogens worldwide in recent years. Pantoea ananatis was reported as the cause of maize white spot, a serious maize disease in Brazil, causing significant yield losses. However, very little information is available about how to detect this pathogen, its genetic variability and the putative alternative hosts in maize-growing areas. To address these issues, we implemented a rapid and efficient PCR-based method to identify P. ananatis isolated from leaves showing white spot symptoms and evaluated its genetic diversity in maize, sorghum and crabgrass. Of the 29 bacteria isolated from typical water-soaked lesions of white spot disease that produced yellow colonies, 15 isolates were identified as P. ananatis by 16S rDNA sequencing and correctly detected by the PCR reaction, amplifying a specific fragment of the ice nucleation gene (ina). These P. ananatis isolates included 13 from maize, one from sorghum and one from crabgrass, while the other 14 yellow colony isolates were from other bacterial species, including two Pantoea species (Pantoea dispersa and Pantoea agglomerans) that were not amplified by the ina primers. These results indicate that the optimized PCR assay can be used to detect P. ananatis isolated from white spot lesions and could be used as a large-scale and cost-effective method of detecting this pathogen in leaf lesions on maize and other grasses. All isolates were evaluated for hypersensitive response (HR) on tobacco, revealing that some P. ananatis were able to induce HR. The high genetic variability revealed by rep-PCR did not differentiated the P. ananatis isolates based on their hosts or HR reaction. The detection, characterization and diversity of P.ananatis from maize, sorghum and crabgrass in our study can be applied in understanding epidemiology and designing control strategies for maize white spot disease in Brazil.
650 $aDoença de planta
650 $aMancha branca
653 $aDiversidade genética
700 1 $aGOMES, E. A.
700 1 $aSILVA, D. D. da
700 1 $aCOSTA, R. V. da
700 1 $aCOTA, L. V.
700 1 $aPARREIRA, D. F.
700 1 $aSOUZA, I. R. P.
700 1 $aGUIMARAES, C. T.
773 $tJournal of Phytopathology, Berlin$gv. 160, p. 441-448, 2012.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Rondônia. Para informações adicionais entre em contato com cpafro.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Rondônia. |
|
Data corrente: |
28/01/1998 |
|
Data da última atualização: |
10/11/2021 |
|
Autoria: |
SOUZA, M. G.; AZEVEDO, D. M. P. de; MENDES, A. M. |
|
Afiliação: |
Embrapa Rondonia - C.Postal 406, CEP. 78900-000, Porto Velho, RO. |
|
Título: |
Avaliacao de linhagens de arroz resistentes a mancha de graos. |
|
Ano de publicação: |
1997 |
|
Fonte/Imprenta: |
In: ENCONTRO DE PESQUISADORES DE RONDONIA, 3., 1997, Porto Velho. Resumos... Porto Velho: UNIR, 1997. p.16. |
|
Idioma: |
Português |
|
Palavras-Chave: |
Brasil; Control; Controle; Disease; Drechslena oryzae; Phomasorghina; Rondonia; Vilhena. |
|
Thesagro: |
Arroz Sequeiro; Doença; Mancha do Grão; Oryza Sativa. |
|
Thesaurus NAL: |
rice. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00788naa a2200289 a 4500
001 1699948
005 2021-11-10
008 1997 bl uuuu u00u1 u #d
100 1 $aSOUZA, M. G.
245 $aAvaliacao de linhagens de arroz resistentes a mancha de graos.
260 $c1997
650 $arice
650 $aArroz Sequeiro
650 $aDoença
650 $aMancha do Grão
650 $aOryza Sativa
653 $aBrasil
653 $aControl
653 $aControle
653 $aDisease
653 $aDrechslena oryzae
653 $aPhomasorghina
653 $aRondonia
653 $aVilhena
700 1 $aAZEVEDO, D. M. P. de
700 1 $aMENDES, A. M.
773 $tIn: ENCONTRO DE PESQUISADORES DE RONDONIA, 3., 1997, Porto Velho. Resumos... Porto Velho: UNIR, 1997. p.16.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Rondônia (CPAF-RO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
