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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
19/07/2023 |
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Data da última atualização: |
19/07/2023 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
AZEVEDO, T. C.; GUIMARAES, F. F. M.; TRINDADE, R. dos S.; LANA, U. G. de P.; SOUZA, I. R. P. de. |
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Afiliação: |
TÁCILA CRISTINA AZEVEDO, Universidade Federal de São João del-Rei; FLÁVIA FERREIRA MENDES GUIMARAES, Faculdades Ciências da Vida; ROBERTO DOS SANTOS TRINDADE, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; ISABEL REGINA PRAZERES DE SOUZA, CNPMS. |
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Título: |
Comparison of protocols for chromosome duplication in haploid maize. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Revista Brasileira de Milho e Sorgo, v. 22, e1311, 2023. |
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DOI: |
https://doi.org/10.18512/rbms2023v22e1311 |
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Idioma: |
Inglês |
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Conteúdo: |
Different methods of chromosome duplication are used to obtain doubled haploids in maize; however, comparisons of efficiency among protocols have only begun to appear in the literature. Therefore, this study aimed to compare the efficiency of three protocols of chromosome duplication. A randomized block experimental design was used, with three replications composed of three treatments of chromosome duplication (duplication by colchicine injection in plants, duplication by immersion of roots in colchicine solution, and duplication by immersion of newly germinated seedlings in colchicine solution). Two hundred seventy haploid seeds were obtained from crossing the genotype source 91500212 with the haploid inducer hybrid Tail P1 × Tail P2, with 90 seeds for each treatment. In the chromosome duplication through injection, a colchicine solution at 0.125% was applied to the stem of the seedlings 15 days after germination. In the chromosome duplication through root immersion, 15 days after germination, the roots of the seedlings were immersed in a 1% colchicine solution for six hours, followed by removing colchicine residues and transplanting them in trays with the substrate. Finally, in the chromosome duplication of newly germinated seedlings, three days old seedlings were immersed in a 0.06% colchicine solution for 12 hours, then removed from the solution and transplanted in trays with the substrate. Twenty days after treatment, the plants were transplanted in 20-liter pots with fertilized soil in a greenhouse, beginning data collection. Results indicate that the injection protocol showed better survival, better practicality of application, and lower costs and risks. MenosDifferent methods of chromosome duplication are used to obtain doubled haploids in maize; however, comparisons of efficiency among protocols have only begun to appear in the literature. Therefore, this study aimed to compare the efficiency of three protocols of chromosome duplication. A randomized block experimental design was used, with three replications composed of three treatments of chromosome duplication (duplication by colchicine injection in plants, duplication by immersion of roots in colchicine solution, and duplication by immersion of newly germinated seedlings in colchicine solution). Two hundred seventy haploid seeds were obtained from crossing the genotype source 91500212 with the haploid inducer hybrid Tail P1 × Tail P2, with 90 seeds for each treatment. In the chromosome duplication through injection, a colchicine solution at 0.125% was applied to the stem of the seedlings 15 days after germination. In the chromosome duplication through root immersion, 15 days after germination, the roots of the seedlings were immersed in a 1% colchicine solution for six hours, followed by removing colchicine residues and transplanting them in trays with the substrate. Finally, in the chromosome duplication of newly germinated seedlings, three days old seedlings were immersed in a 0.06% colchicine solution for 12 hours, then removed from the solution and transplanted in trays with the substrate. Twenty days after treatment, the plants were transplanted in 20-liter pots with f... Mostrar Tudo |
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Palavras-Chave: |
Duplo haploide; Production of lines. |
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Thesagro: |
Linhagem; Milho; Zea Mays. |
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Thesaurus Nal: |
Doubled haploids. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1155039/1/Comparison-of-protocols-for-chromosome-duplication-in-haploid-maize.pdf
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Marc: |
LEADER 02438naa a2200253 a 4500
001 2155039
005 2023-07-19
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.18512/rbms2023v22e1311$2DOI
100 1 $aAZEVEDO, T. C.
245 $aComparison of protocols for chromosome duplication in haploid maize.$h[electronic resource]
260 $c2023
520 $aDifferent methods of chromosome duplication are used to obtain doubled haploids in maize; however, comparisons of efficiency among protocols have only begun to appear in the literature. Therefore, this study aimed to compare the efficiency of three protocols of chromosome duplication. A randomized block experimental design was used, with three replications composed of three treatments of chromosome duplication (duplication by colchicine injection in plants, duplication by immersion of roots in colchicine solution, and duplication by immersion of newly germinated seedlings in colchicine solution). Two hundred seventy haploid seeds were obtained from crossing the genotype source 91500212 with the haploid inducer hybrid Tail P1 × Tail P2, with 90 seeds for each treatment. In the chromosome duplication through injection, a colchicine solution at 0.125% was applied to the stem of the seedlings 15 days after germination. In the chromosome duplication through root immersion, 15 days after germination, the roots of the seedlings were immersed in a 1% colchicine solution for six hours, followed by removing colchicine residues and transplanting them in trays with the substrate. Finally, in the chromosome duplication of newly germinated seedlings, three days old seedlings were immersed in a 0.06% colchicine solution for 12 hours, then removed from the solution and transplanted in trays with the substrate. Twenty days after treatment, the plants were transplanted in 20-liter pots with fertilized soil in a greenhouse, beginning data collection. Results indicate that the injection protocol showed better survival, better practicality of application, and lower costs and risks.
650 $aDoubled haploids
650 $aLinhagem
650 $aMilho
650 $aZea Mays
653 $aDuplo haploide
653 $aProduction of lines
700 1 $aGUIMARAES, F. F. M.
700 1 $aTRINDADE, R. dos S.
700 1 $aLANA, U. G. de P.
700 1 $aSOUZA, I. R. P. de
773 $tRevista Brasileira de Milho e Sorgo$gv. 22, e1311, 2023.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 227 | |
| 82. |  | GONÇALVES, A. B. da S.; NOGUEIRA, R. A.; GOMES, E. A.; LANA, U. G. de P.; SOUSA, S. M. de; GUIMARAES, C. T. Colonização e diversidade genética de fungos micorrízicos arbusculares em linhagens de milho contrastantes para a eficiência no uso de P. In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA PIBIC/BIC JÚNIOR, 12., 2017, Sete Lagoas. [Trabalhos apresentados]. Sete Lagoas: Embrapa Milho e Sorgo, 2017.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 83. | | PAIVA, A. P. L. de; MAGALHAES, P. C.; CARVALHO, L. P. de; JALES, H. F.; GOMES JÚNIOR, C. C.; LANA, U. G. de P.; MARRIEL, I. E. Azospirillum brasilense innoculation combined with different levels of nitrogen fertilization and its effects in the grain sorghum subjected to water restriction. Revista Brasileira de Milho e Sorgo, v. 20, e1215, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 84. | | PAIVA, A. P. L. de; MAGALHAES, P. C.; CARVALHO, L. P. de; JALES, H. F.; GOMES JÚNIOR, C. C.; LANA, U. G. de P.; MARRIEL, I. E. Azospirillum brasilense para mitigação do estresse hídrico no sorgo BRS 332 submetido a diferentes doses de nitrogênio. Sete Lagoas: Embrapa Milho e Sorgo, 2021. 36 p. (Embrapa Milho e Sorgo. Boletim de Pesquisa e Desenvolvimento, 224). ODS 2, ODS 12, ODS 13.| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 85. | | SOUZA, I. R. P. de; GUILHEN, J. H. S.; ANDRADE, C. de L. T. de; PINTO, M. de O.; LANA, U. G. de P.; PASTINA, M. M. Major effect qtl on chromosome 3 conferring maize resistance to Sugarcane mosaic virus. Revista Brasileira de Milho e Sorgo, v. 18, n. 3, p. 322-339, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 87. | | GODINHO, B. T. V.; TAVARES, A. N. G.; RIBEIRO, V. P.; LANA, U. G. de P.; GOMES, E. A.; PFENNING, L. H. Isolamento e seleção de cepas Bacillus spp. para promoção do crescimento vegetal sob condição de estresse hídrico. In: CONGRESSO NACIONAL DE MILHO E SORGO, 32., 2018, Lavras. Soluções integradas para os sistemas de produção de milho e sorgo no Brasil: resumos. Sete Lagoas: Associação Brasileira de Milho e Sorgo, 2018. p. 8.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 88. | | GOMES, E. A.; OLIVEIRA-PAIVA, C. A.; LANA, U. G. de P.; NODA, R. W.; MARRIEL, I. E.; SOUZA, F. A. de. Influência do pH do solo na comunidade de fungos micorrízicos arbusculares associada a raízes de milho contrastantes para eficiência no uso de fósforo. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 26., 2011, Foz do Iguaçu. Resumos. Foz do Iguaçu: Sociedade Brasileira de Microbiologia, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 91. | | OLIVEIRA, R. G. de; CAMPOLINO, M. L.; GOMES, E. A.; SANTOS, F. C. dos; LANA, U. G. de P.; OLIVEIRA-PAIVA, C. A.; SOUSA, S. M. de. Inoculantion effect of phosphate-solubilizing bacteria on microbiota of mayze rhizosphere cultivated with different phosphate fertilization conditions. In: PHOSPHORUS IN SOILS AND PLANTS SYMPOSIUM, 7., 2022, Montevideo. Towards a sustainable phosphorus utilization in agroecosystems: book of abstracts. Montevideo: Instituto Nacional de Investigación Agropecuaria, 2022. p. 44.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 95. | | FIGUEIREDO, J. E. F.; GOMES, E. A.; GUIMARAES, C. T.; LANA, U. G. de P.; TEIXEIRA, M. A.; LIMA, G. V. C.; BRESSAN, W. Molecular analysis endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.). Brazilian Journal of Microbiology, São Paulo, v. 40, n. 3, p. 522-534, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 96. | | LIU, J.; MAGALHAES, J. V. de; GUIMARAES, C. T.; LANA, U. G. de P.; HOEKENGA, O.; SHAFF, J.; PINEROS, M.; WANG, Y.; SCHAFFERT, R. E.; KOCHIAN, L. Molecular cloning and characterization of AltSB, a major aluminum tolerance gene in sorghum. In: INTERNATIONAL PLANT & ANIMAL GENOMES CONFERENCE, 15., 2007, San Diego, CA. [Proceedings...]. [S. l.: s.n.], 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 97. | | PARREIRA, D. F.; ZAMBOLIM, L.; GOMES, E. A.; COSTA, R. V. da; SILVA, D. D. da; COTA, L. V.; LANA, U. G. de P.; SILVA, E. C. F. Estimativa da diversidade genética de Colletotrichum graminicola com marcadores ISSR. In: CONGRESSO NACIONAL DE MILHO E SORGO, 28.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA DO CARTUCHO, 4., 2010, Goiânia. Potencialidades, desafios e sustentabilidade: resumos expandidos... Sete Lagoas: ABMS, 2010. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 98. | | RIBEIRO, V. P.; GOMES, E. A.; SOUSA, S. M. de; LANA, U. G. de P.; COELHO, A. M.; MARRIEL, I. E.; OLIVEIRA-PAIVA, C. A. Co-inoculation with tropical strains of Azospirillum and Bacillus is more efficient than single inoculation for improving plant growth and nutrient uptake in maize. Archives of Microbiology, v. 204, article 143, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 227 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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