Data/hora: 24/06/2024 06:01:29

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 20/10/1995
Data da última atualização: | 20/10/1995
Autoria: | LURO, F.; LAIGRET, F.; BOVE, J. M.; OLLITRAULT, P.
Afiliação: | Station de Recherche Agronomique de Corse, Institut National de la Recherche Agonomique, et al. Laboratoire de Biologie Celluaire et Moleculaire.
Título: | DNA amplified fingerprinting, a useful tool for determination of genetic Origin and diversity analysis in citrus.
Ano de publicação: | 1995
Fonte/Imprenta: | HortScience, v.30, n.5, p.1063-1067, 1995.
Idioma: | Inglês
Conteúdo: | We used three short repetitive nucleotide sequences [(GTG)5, (TCC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted with restricted citrus DNA or as single primers in polymerase chain reaction amplication experiments with total genomic DNA. We tested the ability of the sequences to discriminate between seedlings of zygotic or nucellar origin in the progeny of a Volkamer lemon (Citrus volkameriana Ten. & Pasq.) tree. The genetic variability within two species [Citrus sinensis (L) Osbeck (sweet oranges) and Citrus reticulata Blanco and relatives (mandarins)] also was evaluated. DNA amplified fingerprinting with single primers was the  more successful technique fordiscriminating between nucellar and zygotic seedlings. Although we were not able to distinguish among 10 cultivars of C. sinensis, all 10 reticulata cultivars tested were distinguishable. However, it still is difficult to indentiy the putative parents of a hybrid plant when the two parental genomes are closely related.
Palavras-Chave: | PCR; RAPD.
Thesaurus Nal: | plant breeding; polymorphism.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (3462-1) - ADC - AP - -- - 635.05 -  - 635.05 - DIS