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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/04/1999 |
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Data da última atualização: |
12/04/1999 |
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Autoria: |
BAURENS, F.-C.; NOYER, J.-L.; LANAUD, C.; LAGODA, P. J. L. |
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Afiliação: |
CIRAD. |
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Título: |
Use of competitive PCR to assay copy number of repetitive elements in banana |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Mol. Gen. Genet, v.253, p.57-64, 1996 |
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Idioma: |
Inglês |
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Conteúdo: |
Banana is one of the most important sub-tropical fruit crops. Genetic improvement by traditional breeding strategies is difficult and better knowledge of genomic structure is needed. Repeated sequences are powerful markers for genetic fingerprinting. The method proposed here to determine the copy number of nuclear repetitive elements is based on competitive reverse transcription-polymerase chain reaction and can also be used for quantifying cytosolic sequences. The reliability of this method was investigated on crude preparations of total DNA. Variations due to the heterogeneity of crude DNA extracts showed that a single locus reference is needed for accurate quantification. A mapped microsatellite locus was used to normalize copy number measurements. |
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Palavras-Chave: |
Copy number; DNA repetitivo; Musa-competitive PCR; Numero de copias; PCR competitivo; Repeated DNA. |
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Thesagro: |
Banana. |
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Thesaurus Nal: |
Musa. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01422naa a2200253 a 4500
001 1648090
005 1999-04-12
008 1996 bl --- 0-- u #d
100 1 $aBAURENS, F.-C.
245 $aUse of competitive PCR to assay copy number of repetitive elements in banana
260 $c1996
520 $aBanana is one of the most important sub-tropical fruit crops. Genetic improvement by traditional breeding strategies is difficult and better knowledge of genomic structure is needed. Repeated sequences are powerful markers for genetic fingerprinting. The method proposed here to determine the copy number of nuclear repetitive elements is based on competitive reverse transcription-polymerase chain reaction and can also be used for quantifying cytosolic sequences. The reliability of this method was investigated on crude preparations of total DNA. Variations due to the heterogeneity of crude DNA extracts showed that a single locus reference is needed for accurate quantification. A mapped microsatellite locus was used to normalize copy number measurements.
650 $aMusa
650 $aBanana
653 $aCopy number
653 $aDNA repetitivo
653 $aMusa-competitive PCR
653 $aNumero de copias
653 $aPCR competitivo
653 $aRepeated DNA
700 1 $aNOYER, J.-L.
700 1 $aLANAUD, C.
700 1 $aLAGODA, P. J. L.
773 $tMol. Gen. Genet$gv.253, p.57-64, 1996
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| 186. |  | GRIGOLETTI JUNIOR, A.; SANTOS, A. F. dos; AUER, C. G. Perspectivas do uso do controle biológico contra doenças florestais. Floresta, Curitiba, v. 30, n. 1/2, p. 155-165, jun./dez. 2000. Edição dos Anais do Seminário sobre Proteção Florestal: Incêndios, Pragas, e Doenças.| Biblioteca(s): Embrapa Florestas. |
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