Data/hora: 22/09/2024 16:39:49

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	12/04/1999	
Data da última atualização: 	12/04/1999	
Autoria: 	BAURENS, F.-C.; NOYER, J.-L.; LANAUD, C.; LAGODA, P. J. L.	
Afiliação: 	CIRAD.	
Título: 	Use of competitive PCR to assay copy number of repetitive elements in banana	
Ano de publicação: 	1996	
Fonte/Imprenta: 	Mol. Gen. Genet, v.253, p.57-64, 1996	
Idioma: 	Inglês	
Conteúdo: 	Banana is one of the most important sub-tropical fruit crops. Genetic improvement by traditional breeding strategies is difficult and better knowledge of genomic structure is needed. Repeated sequences are powerful markers for genetic fingerprinting. The method proposed here to determine the copy number of nuclear repetitive elements is based on competitive reverse transcription-polymerase chain reaction and can also be used for quantifying cytosolic sequences. The reliability of this method was investigated on crude preparations of total DNA. Variations due to the heterogeneity of crude DNA extracts showed that a single locus reference is needed for accurate quantification. A mapped microsatellite locus was used to normalize copy number measurements.	
Palavras-Chave: 	Copy number; DNA repetitivo; Musa-competitive PCR; Numero de copias; PCR competitivo; Repeated DNA.	
Thesagro: 	Banana.	
Thesaurus Nal: 	Musa.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (17121-1) - ADD - SP - -- - S11345 -  - S11345 - DIS