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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
30/04/2003 |
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Data da última atualização: |
13/03/2017 |
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Autoria: |
FIGUEIREDO, J. F.; PELLEGRIN, A. O.; FOSCOLO, C. B.; MACHADO, R. P.; MIRANDA, K. L.; LAGE, A. P. |
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Afiliação: |
UFMG/EV (Belo Horizonte, MG); EMBRAPA Pantanal (Corumba, MS). |
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Título: |
Evaluation of direct fluorescent antibody test for the diagnosis of Bovine Genital Campylobacteriosis. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Revista Latinoamericana de Microbiologia, v.44, n.3/4, p.118-123, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
The direct fluorescent antibody test (DFAT) for the diagnosis of Bovine Genital Campylo- bacteriosis was assessed for its detectgion limit, observer effect, sensitivity and specificity. In addition, the specificity of the fluorescent conjugate was tested against Campylobacter sp., Arcobacter sp., Helicobacter sp., E. coli and other bacteria from the preputial flora. Ten - fold dilutions of C. fetus subs. venerealis NCTC 10354 in PBS or preputial washing with or without centrifugation were used. All experiments were done in duplicate by three observers. Positive and negative controls were included in each assay. The detection li- mits of DFAT were 104 CFU/ml for PBS and non - centrifuged preputial washings and 102 CFU/ml for centrifuged preputial washing. There was no observer effect. The sensitivity and specificity of DFAT were 92.59% and 88.89%, respectively. The DFAT was observed to be sensitive, specific and the effect of experienced observers was minimal on test per- formance. |
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Palavras-Chave: |
Antibody test; Bovine genital campylobacteriosis; Campilobacteriosis genital bobina; Diagnosis; Direct fluorescent; Imunofluorescencia direta; Lavado prepucial; Preputial washing. |
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Thesagro: |
Campylobacter Fetus; Diagnostico. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01915naa a2200301 a 4500
001 1810638
005 2017-03-13
008 2002 bl --- 0-- u #d
100 1 $aFIGUEIREDO, J. F.
245 $aEvaluation of direct fluorescent antibody test for the diagnosis of Bovine Genital Campylobacteriosis.
260 $c2002
520 $aThe direct fluorescent antibody test (DFAT) for the diagnosis of Bovine Genital Campylo- bacteriosis was assessed for its detectgion limit, observer effect, sensitivity and specificity. In addition, the specificity of the fluorescent conjugate was tested against Campylobacter sp., Arcobacter sp., Helicobacter sp., E. coli and other bacteria from the preputial flora. Ten - fold dilutions of C. fetus subs. venerealis NCTC 10354 in PBS or preputial washing with or without centrifugation were used. All experiments were done in duplicate by three observers. Positive and negative controls were included in each assay. The detection li- mits of DFAT were 104 CFU/ml for PBS and non - centrifuged preputial washings and 102 CFU/ml for centrifuged preputial washing. There was no observer effect. The sensitivity and specificity of DFAT were 92.59% and 88.89%, respectively. The DFAT was observed to be sensitive, specific and the effect of experienced observers was minimal on test per- formance.
650 $aCampylobacter Fetus
650 $aDiagnostico
653 $aAntibody test
653 $aBovine genital campylobacteriosis
653 $aCampilobacteriosis genital bobina
653 $aDiagnosis
653 $aDirect fluorescent
653 $aImunofluorescencia direta
653 $aLavado prepucial
653 $aPreputial washing
700 1 $aPELLEGRIN, A. O.
700 1 $aFOSCOLO, C. B.
700 1 $aMACHADO, R. P.
700 1 $aMIRANDA, K. L.
700 1 $aLAGE, A. P.
773 $tRevista Latinoamericana de Microbiologia$gv.44, n.3/4, p.118-123, 2002.
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Registro original: |
Embrapa Pantanal (CPAP) |
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| 1. |  | PINTO, T. M. F.; MOREIRA, R. F.; MATOS, M. N. C.; SOARES, V. V. M.; AGUIAR, M. V. de A.; ARAGÃO, P. de T. T. D. de; ALVES FILHO, J. G.; MORENO, F. B. M. B.; MONTEIRO-MOREIRA, A. C. de O.; COSTA, C. R. R.; LIMA FILHO, J. L. de; ELOY, A. M. X.; CUNHA, R. M. S. da. Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus). Animal Reproduction, v. 16, n. 4, p. 902-913, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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