| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
22/02/2013 |
|
Data da última atualização: |
07/03/2023 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso |
|
Autoria: |
CARVALHO, J. O.; MICHALCZECHEN-LACERDA, V. A.; SARTORI, R.; RODRIGUES, F. C.; BRAVIM, O.; FRANCO, M. M.; DODE, M. A. N. |
|
Afiliação: |
JOSE O. CARVALHO, UNIVERSIDADE DE SÃO PAULO; VALQUIRIA A. MICHALCZECHEN-LACERDA, UnB; ROBERTO SARTORI, UNIVERSIDADE DE SÃO PAULO; FERNANDA C. RODRIGUES; OTAVIO BRAVIM, UnB; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
|
Título: |
The methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow-cytometric sex sorting. |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
Molecular Reproduction e Development, v. 79, p. 77-84, 2012. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The objectives of this study were to investigate the effect of sexing by flow cytometry on the methylation patterns of the IGF2 and IGF2R genes. Frozen-thawed, unsorted, and sex-sorted spermsamples fromfour Nellore bulls were used. Each ejaculate was separated into three fractions: non-sexed (NS), sexed forX-sperm(SX), and sexed for Y-sperm (SY). Sperm were isolated from the extender, cryoprotectant, and other cell types by centrifugation on a 40:70%Percoll gradient, and spermpellets were used for genomic DNA isolation. DNA was used for analyses of the methylation patterns by bisul?te sequencing.Methylation status of the IGF2 and IGF2Rgeneswere evaluated by sequencing 195 and 147 individual clones, respectively. No global differences in DNAmethylation were found between NS, SX, and SY groups for the IGF2 (P¼0.09) or IGF2R genes (P¼0.38). Very speci?c methylation patterns were observed in the 25th and 26th CpG sites in the IGF2R gene. representing higher methylation in NS than in the SX and SY groups compared with the other CpG sites. Further, individual variation in methylation patterns was found among bulls. In conclusion, the sex-sorting procedure by flow cytometry did not affect the overall DNA methylation patterns of the IGF2 and IGF2R genes, although individual variation in their methyla-tion patterns among bulls was observed. |
|
Palavras-Chave: |
Gene IGF2; Gene IGF2R; Sexagem por citometria. |
|
Thesagro: |
Bovino. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179337/1/Carvalho-et-al-2012-Molecular-Reproduction-and-Development.pdf
|
|
Marc: |
LEADER 02074nam a2200229 a 4500
001 1950725
005 2023-03-07
008 2012 bl uuuu u00u1 u #d
100 1 $aCARVALHO, J. O.
245 $aThe methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow-cytometric sex sorting.$h[electronic resource]
260 $aMolecular Reproduction e Development, v. 79, p. 77-84$c2012
520 $aThe objectives of this study were to investigate the effect of sexing by flow cytometry on the methylation patterns of the IGF2 and IGF2R genes. Frozen-thawed, unsorted, and sex-sorted spermsamples fromfour Nellore bulls were used. Each ejaculate was separated into three fractions: non-sexed (NS), sexed forX-sperm(SX), and sexed for Y-sperm (SY). Sperm were isolated from the extender, cryoprotectant, and other cell types by centrifugation on a 40:70%Percoll gradient, and spermpellets were used for genomic DNA isolation. DNA was used for analyses of the methylation patterns by bisul?te sequencing.Methylation status of the IGF2 and IGF2Rgeneswere evaluated by sequencing 195 and 147 individual clones, respectively. No global differences in DNAmethylation were found between NS, SX, and SY groups for the IGF2 (P¼0.09) or IGF2R genes (P¼0.38). Very speci?c methylation patterns were observed in the 25th and 26th CpG sites in the IGF2R gene. representing higher methylation in NS than in the SX and SY groups compared with the other CpG sites. Further, individual variation in methylation patterns was found among bulls. In conclusion, the sex-sorting procedure by flow cytometry did not affect the overall DNA methylation patterns of the IGF2 and IGF2R genes, although individual variation in their methyla-tion patterns among bulls was observed.
650 $aBovino
653 $aGene IGF2
653 $aGene IGF2R
653 $aSexagem por citometria
700 1 $aMICHALCZECHEN-LACERDA, V. A.
700 1 $aSARTORI, R.
700 1 $aRODRIGUES, F. C.
700 1 $aBRAVIM, O.
700 1 $aFRANCO, M. M.
700 1 $aDODE, M. A. N.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Roraima. Para informações adicionais entre em contato com cpafrr.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Roraima. |
|
Data corrente: |
27/02/2009 |
|
Data da última atualização: |
18/04/2012 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
|
Autoria: |
SANTOS, F. C. dos; ALBUQUERQUE FILHO, M. R. de; FERREIRA, G. B.; CARVALHO, M. C. S.; SILVA FILHO, J. L. da; PEDROSA, M. B.; SANTOS, J. B. dos; FREIRE, R. M. M.; ALENCAR, A. R. de. |
|
Afiliação: |
Flávia Cristina dos Santos, CPAC; Manuel Ricardo de Albuquerque Filho, CPAC; Gilvan Barbosa Ferreira, CPAF-RR; Maria Conceição Santana Carvalho, CNPA; João Luis da Silva Filho, CNPA; Murilo Barbosa Pedrosa, Fundação Bahia; João Batista dos Santos, EBDA; Rosa Maria Mendes Freire, CNPA; Arnaldo Rocha de Alencar, CNPA. |
|
Título: |
Adubação de manutenção com nitrogênio e enxofre para o algodoeiro cultivado em solo arenoso do Cerrado baiano. |
|
Ano de publicação: |
2008 |
|
Fonte/Imprenta: |
In: SIMPÓSIO NACIONAL CERRADO, 9.; SIMPÓSIO INTERNACIONAL SAVANAS TROPICAIS, 2., 2008, Brasília, DF. Desafios e estratégias para o equilíbrio entre sociedade, agronegócio e recursos naturais: anais... Planaltina, DF: Embrapa Cerrados, 2008. |
|
Idioma: |
Português |
|
Palavras-Chave: |
Nutrição de planta; Qualidade de fibra. |
|
Thesagro: |
Algodão; Fertilidade do Solo. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00986naa a2200253 a 4500
001 1694809
005 2012-04-18
008 2008 bl --- 0-- u #d
100 1 $aSANTOS, F. C. dos
245 $aAdubação de manutenção com nitrogênio e enxofre para o algodoeiro cultivado em solo arenoso do Cerrado baiano.
260 $c2008
650 $aAlgodão
650 $aFertilidade do Solo
653 $aNutrição de planta
653 $aQualidade de fibra
700 1 $aALBUQUERQUE FILHO, M. R. de
700 1 $aFERREIRA, G. B.
700 1 $aCARVALHO, M. C. S.
700 1 $aSILVA FILHO, J. L. da
700 1 $aPEDROSA, M. B.
700 1 $aSANTOS, J. B. dos
700 1 $aFREIRE, R. M. M.
700 1 $aALENCAR, A. R. de
773 $tIn: SIMPÓSIO NACIONAL CERRADO, 9.; SIMPÓSIO INTERNACIONAL SAVANAS TROPICAIS, 2., 2008, Brasília, DF. Desafios e estratégias para o equilíbrio entre sociedade, agronegócio e recursos naturais: anais... Planaltina, DF: Embrapa Cerrados, 2008.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Roraima (CPAF-RR) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
