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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
22/03/2016 |
Data da última atualização: |
19/06/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PÉREZ-MONTAÑO, F.; CERRO, P. del; JIMÉNEZ-GUERRERO, I.; LÓPEZ-BAENA, F. J.; CUBO, M. T.; HUNGRIA, M.; MEGÍAS, M.; OLLERO, F. J. |
Afiliação: |
Francisco Péres-Montaño, Universidade de Sevilla; Pablo del Cerro, Universidade de Sevilia; Irene Jiménez-Guerreiro, Universidade de Sevilla; Francisco Javier López-Baena, Universidade de Sevilla; maria Teresa Cubo, Universidade de Sevilla; MARIANGELA HUNGRIA DA CUNHA, CNPSO; Manuel Megías, Universidade de Sevilla; Francisco Javier Ollero, Uiversidade de Sevilla. |
Título: |
RNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
BMC Genomics, v. 17, n. 1, p. 198, Mar. 2016. |
ISSN: |
1471-2164 |
DOI: |
10.1186/s12864-016-2543-3 |
Idioma: |
Inglês |
Conteúdo: |
Rhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the expression of nodulation genes in the absence of flavonoids. Thus, in the presence of both nodulation inducer molecules, apigenin and salt, R. tropici CIAT 899 up-regulated the same set of symbiotic genes. It could be possible that the increases in the transcription levels of several genes related to nodulation under saline conditions could represent a strategy to establish symbiosis under abiotic stressing conditions. MenosRhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the e... Mostrar Tudo |
Palavras-Chave: |
Lipochitooligosaccharides; Nod factors; Nodutlation; RNA-seq. |
Thesagro: |
Nodulação; Rhizobium. |
Thesaurus Nal: |
Apigenin; Rhizobium tropici; Salt stress. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142584/1/RNA-seq-analysis-of-the-rhizobium...pdf
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Marc: |
LEADER 02939naa a2200337 a 4500 001 2041600 005 2017-06-19 008 2016 bl uuuu u00u1 u #d 022 $a1471-2164 024 7 $a10.1186/s12864-016-2543-3$2DOI 100 1 $aPÉREZ-MONTAÑO, F. 245 $aRNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt.$h[electronic resource] 260 $c2016 520 $aRhizobium tropici strain CIAT 899 establishes effective symbioses with several legume species, including Phaseolus vulgaris and Leucaena leucocephala. This bacterium synthesizes a large variety of nodulation factors in response to nod-gene inducing flavonoids and, surprisingly, also under salt stress conditions. The aim of this study was to identify differentially expressed genes in the presence of both inducer molecules, and analyze the promoter regions located upstream of these genes. Results obtained by RNA-seq analyses of CIAT 899 induced with apigenin, a nod gene-inducing flavonoid for this strain, or salt allowed the identification of 19 and 790 differentially expressed genes, respectively. Fifteen of these genes were up-regulated in both conditions and were involved in the synthesis of both Nod factors and indole-3-acetic acid. Transcription of these genes was presumably activated through binding of at least one of the five NodD proteins present in this strain to specific nod box promoter sequences when the bacterium was induced by both apigenin and salt. Finally, under saline conditions, many other transcriptional responses were detected, including an increase in the transcription of genes involved in trehalose catabolism, chemotaxis and protein secretion, as well as ribosomal genes, and a decrease in the transcription of genes involved in transmembrane transport. To our knowledge this is the first time that a transcriptomic study shows that salt stress induces the expression of nodulation genes in the absence of flavonoids. Thus, in the presence of both nodulation inducer molecules, apigenin and salt, R. tropici CIAT 899 up-regulated the same set of symbiotic genes. It could be possible that the increases in the transcription levels of several genes related to nodulation under saline conditions could represent a strategy to establish symbiosis under abiotic stressing conditions. 650 $aApigenin 650 $aRhizobium tropici 650 $aSalt stress 650 $aNodulação 650 $aRhizobium 653 $aLipochitooligosaccharides 653 $aNod factors 653 $aNodutlation 653 $aRNA-seq 700 1 $aCERRO, P. del 700 1 $aJIMÉNEZ-GUERRERO, I. 700 1 $aLÓPEZ-BAENA, F. J. 700 1 $aCUBO, M. T. 700 1 $aHUNGRIA, M. 700 1 $aMEGÍAS, M. 700 1 $aOLLERO, F. J. 773 $tBMC Genomics$gv. 17, n. 1, p. 198, Mar. 2016.
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Embrapa Soja (CNPSO) |
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Registros recuperados : 4 | |
1. | | CERRO, P. del; PÉREZ-MONTAÑO, F.; GIL-SERRANO, A.; LÓPEZ-BAENA, F. J.; MEGÍAS, M.; HUNGRIA, M.; OLLERO, F. J. The Rhizobium tropici CIAT 899 NodD2 protein regulates the production of Nod factors under salt stress in a flavonoid-independent manner. Scientific Reports, v. 7, n. 46712, May, 2017.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Soja. |
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2. | | DEL-CERRO, P.; PÉREZ-MONTAÑO, F.; JIMÉNEZ-GUERRERO, I.; LÓPEZ BAENA, F. J.; MEGÍAS, M.; HUNGRIA, M.; OLLERO MÁRQUEZ, F. J. RNA-SEQ analysis of rhizobium tropici ciat 899 genome in the presence of apigenin and salt. In: REUNIÃO LATINOAMERICANA DE RIZOBIOLOGIA - RELAR, 27., 2016, Londrina. Fortalecendo as parcerias Sul-Sul: anais. Curitiba: SBCS-NEPAR, 2016. p. 184. Editores: Mariangela Hungria, Douglas Fabiano Gomes, Arnaldo Colozzi Filho.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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3. | | PÉREZ-MONTAÑO, F.; CERRO, P. del; JIMÉNEZ-GUERRERO, I.; LÓPEZ-BAENA, F. J.; CUBO, M. T.; HUNGRIA, M.; MEGÍAS, M.; OLLERO, F. J. RNA-seq analysis of the Rhizobium tropici CIAT 899 Transcripitome shows similarites in the activation patterns of symbiotic genes in the presence of apigenin and salt. BMC Genomics, v. 17, n. 1, p. 198, Mar. 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Soja. |
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4. | | CERRO, P. del; AYALA-GARCÍA, P.; JIMÉNEZ-GUERRERO, I.; LÓPEZ-BAENA, F. J.; VINARDELL, J. M.; MEGÍAS, M.; HUNGRIA, M.; GIL-SERRANO, A. M.; PÉREZ-MONTÃNO, F.; OLLERO, F. J. The non-flavonoid inducible nodA3 and the flavonoid regulated nodA1 genes of Rhizobium tropici CIAT 899 guarantee nod factor production and nodulation of different host legumes. Plant and Soil, v. 440, p. 185-200, 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Soja. |
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Registros recuperados : 4 | |
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