Registro Completo |
Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
25/02/2000 |
Data da última atualização: |
31/03/2017 |
Autoria: |
JAMROZ, R. C.; GUERRERO, F. D.; KAMMLAH, D. M.; KUNZ, S. E. |
Título: |
Role of the kdr and super-kdr sodium channel mutations in pyrethroid resistance: correlation of allelic frequency to resistance level in wild and laboratory populations of horn flies (Haematobia irritans). |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
Insect Biochemistry and Molecular Biology, v.28, p.1031-1037, 1998. |
Idioma: |
Inglês |
Conteúdo: |
The kdr and super-kdr point mutations found in the insect sodium channel gene are postulated to confer knockdown resistance (kdr) to pyrethroids. Using an allele-specific PCR assay to detect these mutations in individual horn flies, Haematobia irritans (L.), we determined ~the allelic frequency of the kdr and super-kdr mutations in several wild and laboratory populations. Wild populations with very similar allelic frequencies had resistance levels that ranged widely from 3- to 18-fold relative toa susceptible population. Conversely, the kdr allele frequency in a lab population with 17-fold resistance was nearly double thar found in a heavily pressured wild population with 18-fold resistance. We conclude that, althought the kdr mutation confers significant levels of pyrethroid resistance, a subtantial component of resistance in insecticidally pressured populations is conferred by mechanisms that are PBO-suppressible. High super-kdr allele frequencies were detected in two resistant lab populations, but in wild populations with equivalent resistance the super-kdr allele frequency was very low. Interestingly, in over 1200 individuals assayed, the super-kdr mutation was never detected in the absence of the kdr mutation. |
Palavras-Chave: |
Canal de sodio; Horn fly; Insect; Mosca-do-chifre; Pyrethroid resistance; Sodium channel. |
Thesagro: |
Haematobia Irritans; Inseto; População. |
Thesaurus Nal: |
population. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02115naa a2200277 a 4500 001 1799784 005 2017-03-31 008 1998 bl --- 0-- u #d 100 1 $aJAMROZ, R. C. 245 $aRole of the kdr and super-kdr sodium channel mutations in pyrethroid resistance$bcorrelation of allelic frequency to resistance level in wild and laboratory populations of horn flies (Haematobia irritans). 260 $c1998 520 $aThe kdr and super-kdr point mutations found in the insect sodium channel gene are postulated to confer knockdown resistance (kdr) to pyrethroids. Using an allele-specific PCR assay to detect these mutations in individual horn flies, Haematobia irritans (L.), we determined ~the allelic frequency of the kdr and super-kdr mutations in several wild and laboratory populations. Wild populations with very similar allelic frequencies had resistance levels that ranged widely from 3- to 18-fold relative toa susceptible population. Conversely, the kdr allele frequency in a lab population with 17-fold resistance was nearly double thar found in a heavily pressured wild population with 18-fold resistance. We conclude that, althought the kdr mutation confers significant levels of pyrethroid resistance, a subtantial component of resistance in insecticidally pressured populations is conferred by mechanisms that are PBO-suppressible. High super-kdr allele frequencies were detected in two resistant lab populations, but in wild populations with equivalent resistance the super-kdr allele frequency was very low. Interestingly, in over 1200 individuals assayed, the super-kdr mutation was never detected in the absence of the kdr mutation. 650 $apopulation 650 $aHaematobia Irritans 650 $aInseto 650 $aPopulação 653 $aCanal de sodio 653 $aHorn fly 653 $aInsect 653 $aMosca-do-chifre 653 $aPyrethroid resistance 653 $aSodium channel 700 1 $aGUERRERO, F. D. 700 1 $aKAMMLAH, D. M. 700 1 $aKUNZ, S. E. 773 $tInsect Biochemistry and Molecular Biology$gv.28, p.1031-1037, 1998.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Pantanal (CPAP) |
|