Data/hora: 30/05/2024 23:30:56

Biblioteca(s): | Embrapa Pantanal.
Data corrente: | 19/04/1996
Data da última atualização: | 03/04/2017
Autoria: | RILEY, D. E.; WAGNER, B.; POLLEY, L.; KRIEGER, J. N.
Título: | PCR-Based study of conserved and variable DNA sequences of Tritrichomonas foetus isolates from Saskatchewan, Canada.
Ano de publicação: | 1995
Fonte/Imprenta: | Journal Clinical Microbiology, v.33, n.5, p.1308-1313, 1995.
Idioma: | Inglês
Conteúdo: | The protozoan parasite Tritrichomonas foetus causes infetility and spontaneous abortion in cattle. In Saskatchevan, Canada, the culture prevalence of trichomonads was 65 of 1,048 (6%) among 1,048 bulls tested within a 1-year period ending in April 1994. Saskatchewan was previously to be free of the parasite. To confirm the culture results, possible T. foetus DNA presence was determined by the PCR. All of the 16 culture-positive isolates tested were PCR positive by a single-band test, but one PCR produt was weak. DNAfingerprinting by both T17 PCR and randomly amplified polymorphic DNA PCR revelated genetic variation or polymorphism among the T. foetus isolates. T17 PCR also revealed conserved loci that distinguished these T. foetus isolates from Trichomonas vaginalis, from a variety of other protozoa, and from prokaryotes. TCO-1 PCR, a PCR test designed to sample DNA sequences homologous to the 5 flank of a highly conservbed cell division control gene, detected genetic polymorphism at low stringency and a conserved, single locus a higher stringency. These findings suggested that T. foetus isolates exhibit both conserved genetic loci and polymorphic loci detectable by independent PCR methos. Both conserved and polymorphic genetic loci may prove useful for improved clinical diagnosis of T. foetus. The polymorphic loci detected by PCR suggested either a long history of infection or multiple lines of T. foetus infection in Saskatchewan. Polymorphic loci detected by PCR may provide data for epidemiologic studies of T. foetus.
Palavras-Chave: | Disease; Tritrichomona.
Thesagro: | Doença; Gado.
Thesaurus Nal: | cattle.
Categoria do assunto: | --
Registro original: | Embrapa Pantanal (CPAP)
Exemplares: | CPAP - (31606-1) - ADC - SP - PP - SP14799 -  - 14799 - DIS