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Registro Completo |
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
27/06/1995 |
Data da última atualização: |
27/06/1995 |
Autoria: |
KRANZ, R. G.; HASELKORN, R. |
Título: |
Ammonia-constitutive nitrogen fixation mutants of Rhodobacter capsulatus. |
Ano de publicação: |
1988 |
Fonte/Imprenta: |
Gene, v.71, p.65-74, 1988. |
Idioma: |
Português |
Palavras-Chave: |
FBN. |
Thesagro: |
Amônia; Mutação. |
Thesaurus Nal: |
Rhodobacter capsulatus. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00444naa a2200169 a 4500 001 1610585 005 1995-06-27 008 1988 bl --- 0-- u #d 100 1 $aKRANZ, R. G. 245 $aAmmonia-constitutive nitrogen fixation mutants of Rhodobacter capsulatus. 260 $c1988 650 $aRhodobacter capsulatus 650 $aAmônia 650 $aMutação 653 $aFBN 700 1 $aHASELKORN, R. 773 $tGene$gv.71, p.65-74, 1988.
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Embrapa Agrobiologia (CNPAB) |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
22/04/2016 |
Data da última atualização: |
10/07/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
CERRO, P. del; ROLLA-SANTOS, A. A. P.; VALDERRAMA-FERNANDEZ, R.; GIL-SERRANO, A.; BELLOGÍN, R. A.; GOMES, D. F.; MONTAÑO, F. P.; MEGÍAS, M.; HUNGRIA, M.; JAVIER OLLERO, F. |
Afiliação: |
PABLO DEL CERRO, Universidad de Sevilla; AMANDA A. P. ROLLA SANTOS, Pós-graduanda; ROCÍO VALDERRAMA FERNANDEZ, Universidad de Sevilla; ANTONIO GIL SERRANO, Universidad de Sevilla; RAMÓN A. BELLOGÍN, Universidad de Sevilla; DOUGLAS FABIANO GOMES, Pós-graduando; FRANCISCO PÉREZ MONTAÑO, Universidad de Sevilla; MANUEL MEGÍAS, Universidad de Sevilla; MARIANGELA HUNGRIA DA CUNHA, CNPSO; FRANCISCO JAVIER OLLERO, Universidad de Sevilla. |
Título: |
NrcR, a new transcriptional regulator of Rhizobium tropici CIAT 899 involved in the Legume root-nodule symbiosis. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
PLOS One, v. 11, n. 4, e0154029, Apr. 2016. |
ISSN: |
1932-6203 |
DOI: |
10.1371/journal.pone.0154029 |
Idioma: |
Inglês |
Conteúdo: |
The establishment of nitrogen-fixing rhizobium-legume symbioses requires a highly complex cascade of events. In this molecular dialogue the bacterial NodD transcriptional regulators in conjunction with plant inducers, mostly flavonoids, are responsible for the biosynthesis and secretion of Nod factors which are key molecules for successful nodulation. Other transcriptional regulators related to the symbiotic process have been identified in rhizobial genomes, including negative regulators such as NolR. Rhizobium tropici CIAT 899 is an important symbiont of common bean (Phaseolus vulgaris L.), and its genome encompasses intriguing features such as five copies of nodD genes, as well as other possible transcriptional regulators including the NolR protein. Here we describe and characterize a new regulatory gene located in the non-symbiotic plasmid pRtrCIAT899c, that shows homology (46% identity) with the nolR gene located in the chromosome of CIAT 899. The mutation of this gene, named nrcR (nolR-like plasmid c Regulator), enhanced motility and exopolysaccharide production in comparison to the wild-type strain. Interestingly, the number and decoration of Nod Factors produced by this mutant were higher than those detected in the wildtype strain, especially under salinity stress. The nrcR mutant showed delayed nodulation and reduced competitiveness with P. vulgaris, and reduction in nodule number and shoot dry weight in both P. vulgaris and Leucaena leucocephala. Moreover, the mutant exhibited reduced capacity to induce the nodC gene in comparison to the wild-type CIAT 899. The finding of a new nod-gene regulator located in a non-symbiotic plasmid may reveal the existence of even more complex mechanisms of regulation of nodulation genes in R. tropici CIAT 899 that may be applicable to other rhizobial species. MenosThe establishment of nitrogen-fixing rhizobium-legume symbioses requires a highly complex cascade of events. In this molecular dialogue the bacterial NodD transcriptional regulators in conjunction with plant inducers, mostly flavonoids, are responsible for the biosynthesis and secretion of Nod factors which are key molecules for successful nodulation. Other transcriptional regulators related to the symbiotic process have been identified in rhizobial genomes, including negative regulators such as NolR. Rhizobium tropici CIAT 899 is an important symbiont of common bean (Phaseolus vulgaris L.), and its genome encompasses intriguing features such as five copies of nodD genes, as well as other possible transcriptional regulators including the NolR protein. Here we describe and characterize a new regulatory gene located in the non-symbiotic plasmid pRtrCIAT899c, that shows homology (46% identity) with the nolR gene located in the chromosome of CIAT 899. The mutation of this gene, named nrcR (nolR-like plasmid c Regulator), enhanced motility and exopolysaccharide production in comparison to the wild-type strain. Interestingly, the number and decoration of Nod Factors produced by this mutant were higher than those detected in the wildtype strain, especially under salinity stress. The nrcR mutant showed delayed nodulation and reduced competitiveness with P. vulgaris, and reduction in nodule number and shoot dry weight in both P. vulgaris and Leucaena leucocephala. Moreover, the mutan... Mostrar Tudo |
Thesagro: |
Fixação de nitrogenio. |
Thesaurus NAL: |
Nitrogen fixation. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142515/1/NrcR-a-new-transcriptional....pdf
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Marc: |
LEADER 02681naa a2200277 a 4500 001 2043848 005 2017-07-10 008 2016 bl uuuu u00u1 u #d 022 $a1932-6203 024 7 $a10.1371/journal.pone.0154029$2DOI 100 1 $aCERRO, P. del 245 $aNrcR, a new transcriptional regulator of Rhizobium tropici CIAT 899 involved in the Legume root-nodule symbiosis.$h[electronic resource] 260 $c2016 520 $aThe establishment of nitrogen-fixing rhizobium-legume symbioses requires a highly complex cascade of events. In this molecular dialogue the bacterial NodD transcriptional regulators in conjunction with plant inducers, mostly flavonoids, are responsible for the biosynthesis and secretion of Nod factors which are key molecules for successful nodulation. Other transcriptional regulators related to the symbiotic process have been identified in rhizobial genomes, including negative regulators such as NolR. Rhizobium tropici CIAT 899 is an important symbiont of common bean (Phaseolus vulgaris L.), and its genome encompasses intriguing features such as five copies of nodD genes, as well as other possible transcriptional regulators including the NolR protein. Here we describe and characterize a new regulatory gene located in the non-symbiotic plasmid pRtrCIAT899c, that shows homology (46% identity) with the nolR gene located in the chromosome of CIAT 899. The mutation of this gene, named nrcR (nolR-like plasmid c Regulator), enhanced motility and exopolysaccharide production in comparison to the wild-type strain. Interestingly, the number and decoration of Nod Factors produced by this mutant were higher than those detected in the wildtype strain, especially under salinity stress. The nrcR mutant showed delayed nodulation and reduced competitiveness with P. vulgaris, and reduction in nodule number and shoot dry weight in both P. vulgaris and Leucaena leucocephala. Moreover, the mutant exhibited reduced capacity to induce the nodC gene in comparison to the wild-type CIAT 899. The finding of a new nod-gene regulator located in a non-symbiotic plasmid may reveal the existence of even more complex mechanisms of regulation of nodulation genes in R. tropici CIAT 899 that may be applicable to other rhizobial species. 650 $aNitrogen fixation 650 $aFixação de nitrogenio 700 1 $aROLLA-SANTOS, A. A. P. 700 1 $aVALDERRAMA-FERNANDEZ, R. 700 1 $aGIL-SERRANO, A. 700 1 $aBELLOGÍN, R. A. 700 1 $aGOMES, D. F. 700 1 $aMONTAÑO, F. P. 700 1 $aMEGÍAS, M. 700 1 $aHUNGRIA, M. 700 1 $aJAVIER OLLERO, F. 773 $tPLOS One$gv. 11, n. 4, e0154029, Apr. 2016.
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