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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
15/03/2017 |
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Data da última atualização: |
15/03/2017 |
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Autoria: |
TATEYAMA, S.; KAWANO, A.; YAMAGUCHI, R.; NOSAKA, D.; KONDO, F. |
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Título: |
Culture conditions and cell morphology of goat milk-derived mammary epithelial cells in plate culture. |
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Ano de publicação: |
1988 |
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Fonte/Imprenta: |
Japanese Journal of Veterinary Science v. 50, n. 6, p. 1192-1199,, Dec. 1988. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: In order to provide a procedure for studying the mechanism of milk production or carcinogenesis of mammary cells, we attempted to culture mammary epithelial cells from goat milk, and estimated the optimal conditions for the culture system. Cell proliferation was accomplished satisfactorily by using Eagle's minimum essential medium supplemented with 30% fetal calf serum (FCS). However, favorable proliferation was not observed when less than 20% FCS was present. In the course of culture, the cells adhered to plastic dishes within 1 or 2 days after seeding and formed small colonies within 5 days. Such colonies continued to increase in size and made contact with neighboring colonies to form sheets within 10 to 20 days. The sheet-forming cells showed various types of cell morphology, including spindle-like, polygonal and stellate forms. At the 5th or 6th passage, the main proportion of the cell population was comprised of elongated cells and pleomorphic cells. Most of the cells were positive for antikeratin and antiactin antiserum. Electron microscopy of vertical sections of the cell sheet revealed that the cells had flat nuclei and extremely extended cytoplasm. They exhibited an apparent epithelial nature in that they were interconnected by interdigitations and possessed microvilli and desmosomes. Morphological features of differentiation of these cultured cells, such as production of casein granules or lipid droplets, were not observed in the present culture system. |
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Palavras-Chave: |
Cultured milk; Diferenciação celular; Epitélio. |
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Thesagro: |
Caprino; Glândula mamária; Leite de cabra; Leite fermentado. |
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Thesaurus Nal: |
Cell differentiation; epithelial cells; Epithelium; Goat milk; Mammary glands. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02383naa a2200313 a 4500
001 2067040
005 2017-03-15
008 1988 bl uuuu u00u1 u #d
100 1 $aTATEYAMA, S.
245 $aCulture conditions and cell morphology of goat milk-derived mammary epithelial cells in plate culture.
260 $c1988
520 $aAbstract: In order to provide a procedure for studying the mechanism of milk production or carcinogenesis of mammary cells, we attempted to culture mammary epithelial cells from goat milk, and estimated the optimal conditions for the culture system. Cell proliferation was accomplished satisfactorily by using Eagle's minimum essential medium supplemented with 30% fetal calf serum (FCS). However, favorable proliferation was not observed when less than 20% FCS was present. In the course of culture, the cells adhered to plastic dishes within 1 or 2 days after seeding and formed small colonies within 5 days. Such colonies continued to increase in size and made contact with neighboring colonies to form sheets within 10 to 20 days. The sheet-forming cells showed various types of cell morphology, including spindle-like, polygonal and stellate forms. At the 5th or 6th passage, the main proportion of the cell population was comprised of elongated cells and pleomorphic cells. Most of the cells were positive for antikeratin and antiactin antiserum. Electron microscopy of vertical sections of the cell sheet revealed that the cells had flat nuclei and extremely extended cytoplasm. They exhibited an apparent epithelial nature in that they were interconnected by interdigitations and possessed microvilli and desmosomes. Morphological features of differentiation of these cultured cells, such as production of casein granules or lipid droplets, were not observed in the present culture system.
650 $aCell differentiation
650 $aepithelial cells
650 $aEpithelium
650 $aGoat milk
650 $aMammary glands
650 $aCaprino
650 $aGlândula mamária
650 $aLeite de cabra
650 $aLeite fermentado
653 $aCultured milk
653 $aDiferenciação celular
653 $aEpitélio
700 1 $aKAWANO, A.
700 1 $aYAMAGUCHI, R.
700 1 $aNOSAKA, D.
700 1 $aKONDO, F.
773 $tJapanese Journal of Veterinary Science$gv. 50, n. 6, p. 1192-1199,, Dec. 1988.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| Registros recuperados : 9 | |
| 4. |  | QUEIROZ, C. B. de; GASPAROTTO, L.; SOUSA, N. R.; SILVA, G. F. da. Desenvolvimento de IRAP (Inter-Retrotransposon Amplified Polymorphism) para Mycosphaerella fijiensis. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. 1 CD-ROM. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 304).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 5. | | PAIXÃO, R. V.; QUEIROZ, C. B. de; GASPAROTTO, L.; SOUSA, N. R.; SILVA, G. F. da. Triagem de iniciadores ISSR para o desenvolvimento de REMAP (Retrotransposon-Microsatellite Amplified Polymorphism) em Micosphaerella fijiensis. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. 1 CD-ROM. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 304).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 6. | | QUEIROZ, C. B. de; MIRANDA, E. C.; GASPAROTTO, L.; HANADA, R. E.; SOUSA, N. R.; SILVA, G. F. da. Análise da população de Mycosphaerella fijiensis por meio do marcador M13 fingerprinting. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS; WORKSHOP EM BIOPROSPECÇÃO E CONSERVAÇÃO DE PLANTAS NATIVAS DO SEMI-ÁRIDO, 3.; WORKSHOP INTERNACIONAL SOBRE BIOENERGIA E MEIO AMBIENTE, 2010, Salvador. Bancos de germoplasma: descobrir a riqueza, garantir o futuro: anais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. 1 CD-ROM. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 304).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 9. | | SOUSA, A.; QUEIROZ, C. B. de; GUALBERTO, G. F.; GASPAROTTO, L.; SOUSA, N. R.; GOMES, P. da C.; SOUZA, R. F. de; HANADA, R. E.; PAIXÃO, R. V.; PAIXÃO, R. V.; SILVA, G. F. da. Diversidade genética e estrutura da população de Mycosphaerella fijiensis no Brasil. Tropical Plant Pathology, Brasília, DF, v. 37, 2012. 1 CD-ROM. Suplemento. Edição do 45º Congresso Brasileiro de Fitopatologia, 2012, Manaus. Mesa redonda 6/39.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| Registros recuperados : 9 | |
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