Data/hora: 23/06/2024 21:08:17

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 06/05/1996
Data da última atualização: | 06/05/1996
Autoria: | KOJIMA, K.; GOTO, A.; YAMADA, Y.
Afiliação: | Kuchinotsu Branch, Fruit Tree Research Station Kuchinotsu, Nagasaki, 859-25, Japan.
Título: | Simultaneous Measurement of ABA, IAA and GAs in Citrus Fruits.
Ano de publicação: | 1995
Fonte/Imprenta: | Bull. Fruit Tree Res. Stn. n.27, p.1-10, 1995
Idioma: | Inglês
Conteúdo: | Method for Simultaneous measurement of ABA, IAA and GAs in citrus fruit (Citrus unshiu Marc. cv. Ozaki) was investigated. When both ether and ethyl acetate extracts from whole fruits were chromatographed by HPLC, almost GAs activity was present in the early eluted fractions. As these fractions were gradually diluted and were applied for the bioassay, GAs activity calculated from a standard curve tended to increase until the threshold for measurement of GAs activity, possibly because the substances that inhibit the GAs activity were diluted. Thus when only the early effluent on HPLC from ether and the bioassay together, the simple assay of total GAs content could be developed. Using this simple procedure, it was observed that within a young citrus fruit 60 daysafter full bloom ABA and IAA were present in pulp and GAs in axis at highest concentrations. Thus, this method should be proved very useful for the simultaneous measurement of ABA, IAA and GAs from one extract of citrus fruits.
Palavras-Chave: | Chromatographed; Indole-3-acetic; simultaneous measerement.
Thesagro: | Ácido Abscisico; Citrus Unshiu; Cromatografia.
Thesaurus Nal: | abscisic acid; Citrus; gibberellins.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (3698-1) - ADC - AP - -- - 634.0405 -  - 634.0405 - DIS