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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
09/01/2019 |
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Data da última atualização: |
24/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MELO, J. O.; MARTINS, L. G. C.; BARROS, B. de A.; PIMENTA, M. R.; LANA, U. G. de P.; DUARTE, C. E. M.; PASTINA, M. M.; GUIMARÃES, C. T.; SCHAFFERT, R. E.; KOCHIAN, L. V.; FONTES, E. P. B.; MAGALHAES, J. V. de. |
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Afiliação: |
Janaina O. Melo, Universidade Federal de Minas Gerais; Laura G. C. Martins, Universidade Federal de Viçosa; BEATRIZ DE ALMEIDA BARROS, CNPMS; Maiana R. Pimenta, Universidade Federal de Viçosa; UBIRACI GOMES DE PAULA LANA, CNPMS; Christiane E. M. Duarte, Universidade Federal de Viçosa; MARIA MARTA PASTINA, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; ROBERT EUGENE SCHAFFERT, CNPMS; Leon V. Kochian, University of Saskatchewan, Canada; Elizabeth P. B. Fontes, Universidade Federal de Viçosa; JURANDIR VIEIRA DE MAGALHAES, CNPMS. |
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Título: |
Repeat variants for the SbMATE transporter protect sorghum roots from aluminum toxicity by transcriptional interplay in cis and trans. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Proceedings of the National Academy of Sciences of the United States of America, Washington, v. 116, n. 1, p. 313-318, 2019. |
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DOI: |
10.1073/pnas.1808400115 |
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Idioma: |
Inglês |
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Notas: |
Publicado online em 13 dez. 2018. |
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Conteúdo: |
Acidic soils, where aluminum (Al) toxicity is a major agricultural constraint, are globally widespreadand are prevalent in developing countries. In sorghum, the root citrate transporter SbMATE confers Al tolerance by protecting root apices from toxic Al3+, but can exhibit reduced expression when introgressed into different lines. We show that allele-specific SbMATE transactivation occurs and is caused by factors located away from SbMATE. Using expressionQTL mapping and expression genome-wide association mapping, we establish that SbMATE transcription is controlled in a bipartite fashion,primarily incis butalsointrans.Multiallelicpromotertransactivation and ChIP analyses demonstrated that intermolecular effects on SbMATE expression arise from a WRKY and a zinc fingerDHHC transcription factor (TF) that bind to and trans-activate the SbMATE promoter. A haplotype analysis in sorghum RILs indicates that the TFs influence SbMATE expression and Al tolerance. Variation in SbMATE expression likely results from changes in tandemly repeated cis sequences flanking a transposable element (a miniature inverted repeattransposable element)insertion inthe SbMATE promoter, which are recognized by the Al3+-responsive TFs. According to our model, repeat expansion in Al-tolerant genotypes increases TF recruitment and, hence, SbMATE expression, which is, in turn, lower in Al-sensitive genetic backgrounds as a result of lower TF expression and fewer binding sites. We thus show that even dominant cis regulation of an agronomically important gene can be subjected to precise intermolecular finetuning. These concerted cis/trans interactions, which allow the plant to sense and respond to environmental cues, such as Al3+ toxicity, can now be used to increase yields and food security on acidic soils. MenosAcidic soils, where aluminum (Al) toxicity is a major agricultural constraint, are globally widespreadand are prevalent in developing countries. In sorghum, the root citrate transporter SbMATE confers Al tolerance by protecting root apices from toxic Al3+, but can exhibit reduced expression when introgressed into different lines. We show that allele-specific SbMATE transactivation occurs and is caused by factors located away from SbMATE. Using expressionQTL mapping and expression genome-wide association mapping, we establish that SbMATE transcription is controlled in a bipartite fashion,primarily incis butalsointrans.Multiallelicpromotertransactivation and ChIP analyses demonstrated that intermolecular effects on SbMATE expression arise from a WRKY and a zinc fingerDHHC transcription factor (TF) that bind to and trans-activate the SbMATE promoter. A haplotype analysis in sorghum RILs indicates that the TFs influence SbMATE expression and Al tolerance. Variation in SbMATE expression likely results from changes in tandemly repeated cis sequences flanking a transposable element (a miniature inverted repeattransposable element)insertion inthe SbMATE promoter, which are recognized by the Al3+-responsive TFs. According to our model, repeat expansion in Al-tolerant genotypes increases TF recruitment and, hence, SbMATE expression, which is, in turn, lower in Al-sensitive genetic backgrounds as a result of lower TF expression and fewer binding sites. We thus show that even dominant c... Mostrar Tudo |
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Palavras-Chave: |
Estresse abiótico; Regulação transcricional; Toxicidade do alumínio. |
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Thesagro: |
Gene; Genética. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02927naa a2200337 a 4500
001 2103451
005 2020-01-24
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1073/pnas.1808400115$2DOI
100 1 $aMELO, J. O.
245 $aRepeat variants for the SbMATE transporter protect sorghum roots from aluminum toxicity by transcriptional interplay in cis and trans.$h[electronic resource]
260 $c2019
500 $aPublicado online em 13 dez. 2018.
520 $aAcidic soils, where aluminum (Al) toxicity is a major agricultural constraint, are globally widespreadand are prevalent in developing countries. In sorghum, the root citrate transporter SbMATE confers Al tolerance by protecting root apices from toxic Al3+, but can exhibit reduced expression when introgressed into different lines. We show that allele-specific SbMATE transactivation occurs and is caused by factors located away from SbMATE. Using expressionQTL mapping and expression genome-wide association mapping, we establish that SbMATE transcription is controlled in a bipartite fashion,primarily incis butalsointrans.Multiallelicpromotertransactivation and ChIP analyses demonstrated that intermolecular effects on SbMATE expression arise from a WRKY and a zinc fingerDHHC transcription factor (TF) that bind to and trans-activate the SbMATE promoter. A haplotype analysis in sorghum RILs indicates that the TFs influence SbMATE expression and Al tolerance. Variation in SbMATE expression likely results from changes in tandemly repeated cis sequences flanking a transposable element (a miniature inverted repeattransposable element)insertion inthe SbMATE promoter, which are recognized by the Al3+-responsive TFs. According to our model, repeat expansion in Al-tolerant genotypes increases TF recruitment and, hence, SbMATE expression, which is, in turn, lower in Al-sensitive genetic backgrounds as a result of lower TF expression and fewer binding sites. We thus show that even dominant cis regulation of an agronomically important gene can be subjected to precise intermolecular finetuning. These concerted cis/trans interactions, which allow the plant to sense and respond to environmental cues, such as Al3+ toxicity, can now be used to increase yields and food security on acidic soils.
650 $aGene
650 $aGenética
653 $aEstresse abiótico
653 $aRegulação transcricional
653 $aToxicidade do alumínio
700 1 $aMARTINS, L. G. C.
700 1 $aBARROS, B. de A.
700 1 $aPIMENTA, M. R.
700 1 $aLANA, U. G. de P.
700 1 $aDUARTE, C. E. M.
700 1 $aPASTINA, M. M.
700 1 $aGUIMARÃES, C. T.
700 1 $aSCHAFFERT, R. E.
700 1 $aKOCHIAN, L. V.
700 1 $aFONTES, E. P. B.
700 1 $aMAGALHAES, J. V. de
773 $tProceedings of the National Academy of Sciences of the United States of America, Washington$gv. 116, n. 1, p. 313-318, 2019.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 53 | |
| 41. |  | SOUSA, S. M. de; CLARK, R.; MENDES, F. F.; OLIVEIRA, A. C. de; KOCHIAN, L. V.; VASCONCELOS, M. J. V.; PARENTONI, S. N.; GUIMARAES, C. T.; MAGALHAES, J. V. Phenotypes and genes associated with root traits to search to phosphorus acquisition efficiency in maize. In: ANNUAL MAIZE GENETICS CONFERENCE, 54., 2011, Portland, Oregon. Program and abstracts... Portland: [s.n.], 2012. p. 156.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 42. | | SOUSA, S. M. de; CLARK, R. T.; MENDES, F. F.; OLIVEIRA, A. C.; KOCHIAN, L. V.; VASCONCELOS, M. J. V.; PARENTONI, S. N.; GUIMARAES, C. T.; MAGALHAES, J. V. Phenotypes and genes associated with root traits underlying phosphorus acquisition efficiency in maize. In: INTERNATIONAL CROP SCIENCE CONGRESS, 6., 2012, Bento Gonçalves. [Proceedings...] [S.l.]: International Crop Science Society, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 43. | | SOUSA, S. M. de; CLARK, R. T.; MENDES, F. F.; OLIVEIRA, A. C. de; VASCONCELOS, M. J. V. de; PARENTONI, S. N.; KOCHIAN, L. V.; GUIMARAES, C. T.; MAGALHAES, J. V. A role for root morphology and related candidate genes in P acquisition efficiency in maize. Functional Plant Biology, Victoria, v. 39, n. 11, p. 925-935, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
|  |
| 44. | | GUIMARAES, C. T.; MAGALHAES, J. V. D.; JARDIM, S. N.; ALMEIDA, R. V.; MARON, L. G.; PAIVA, E.; ALVES, V. M. C.; VIANA, J. M. S.; HOEKENGA, O.; PARENTONI, S. N.; KOCHIAN, L. V. Validation of aluminum tolerance QTL in maize. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 155.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 45. | | MARON, L. G.; GUIMARAES, C. T.; KIRST, M.; ALBERT, P. S.; BIRCHLER, J. A.; BRADBURY, P. J.; BUCKLER, E. S.; COLUCCIO, A. E.; DANILOVA, T. V.; KUDMA, D.; MAGALHAES, J. V.; PIÑEROS, M. A.; SCHATZ, M. C.; WING, R. A.; KOCHIAN, L. V. Aluminum tolerance in maize is associated with higher MATE 1 gene copy number. Proceedings of the National Academy of Sciences of the United States of America, Washington,v. 110, n. 13, p. 5241-5246, Mar. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 46. | | BERNARDINO, K. C.; PASTINA, M. M.; MENEZES, C. B. de; SOUSA, S. M. de; MACIEL, L. S.; CARVALHO JÚNIOR, G.; GUIMARÃES, C. T.; BARROS, B. de A.; SILVA, L. da C. e; CARNEIRO, P. C. S.; SCHAFFERT, R. E.; KOCHIAN, L. V.; MAGALHAES, J. V. de. The genetic architecture of phosphorus efficiency in sorghum involves pleiotropic QTL for root morphology and grain yield under low phosphorus availability in the soil. BMC Plant Biology, v. 19, n. 87, p. 1-15, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 47. | | GUIMARAES, C. T.; SIMOES, C. C.; PASTINA, M. M.; MARON, L. G.; MAGALHAES, J. V.; VASCONCELLOS, R. C. C.; GUIMARAES, L. J. M.; LANA, U. G. de P.; TINOCO, C. F. S.; NODA, R. W.; BELICUAS, S. N. J.; KOCHIAN, L. V.; ALVES, V. M. C.; PARENTONI, S. N. Genetic dissection of Al tolerance QTLs in the maize genome by high density SNP scan. BMC Genomics, v. 15, n. 153, p. 1-14, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 48. | | MAGALHAES, J. V. de; LIU, J.; GUIMARAES, C. T.; LANA, U. G. de P.; ALVES, V. M. C.; WANG, Y-H.; SCHAFFERT, R. E.; HOEKENGA, O. A.; PINEROS, M. A.; SHAFF, J. E.; KLEIN, P. E.; CARNEIRO, N. P.; COELHO, C. M.; TRICK, H. N.; KOCHIAN, L. V. A gene in the multidrug and toxic compound extrusion (MATE) family confers aluminum tolerance in sorghum. Nature Genetics, New York, v. 39, n. 9, p. 1156-1161, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 49. | | KOCHIAN, L. V.; MAGALHAES, J.; GUIMARÃES, C.; ALVES, V.; CARNEIRO, N.; CARNEIRO, A.; SCHAFFERT, R.; BRAMMER, S.; CONSOLI, L.; SILVA JUNIOR, J. P. da; MINELLA, E.; CAIERÃO, E.; NASCIMENTO JUNIOR, A.; BRESEGHELLO, F.; NEVES, P.; GUDU, S.; MARON, L.; HOEKENGA, O.; LIU, J.; BUCKLER, E. Isolation and characterisation of aluminum tolerance genes in the cereals: an integrated functional genomic, molecular genetic and physiological analysis. In: GENERATION CHALLENGE PROGRAMME CULTIVATING PLANT DIVERSITY FOR THE RESOURCE-POOR, 2008, Mexico. Project abstracts... Mexico: Generation Challenge programme, 2008. p. 23-25.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 50. | | KOCHIAN, L. V.; MAGALHAES, J.; GUIMARAES, C.; ALVES, V.; CARNEIRO, N.; CARNEIRO, A.; SCHAFFERT, R.; BRAMMER, S.; CONSOLI, L.; SILVA JUNIOR, J. P. da; MINELLA, E.; CAIERAO, E.; NASCIMENTO JUNIOR, A. do; BRESEGHELLO, F.; NEVES, P.; GUDU, S.; MARON, L.; HOEKENGA, O.; LIU, J.; BUCKLER, E. Isolation and characterisation of aluminum tolerance genes in the cereals: an integrated functional genomic, molecular genetic and physiological analysis. In: GENERATION CHALLENGE PROGRAMME CULTIVATING PLANT DIVERSITY FOR THE RESOURCE-POOR, 2008, Mexico. Project abstracts... Mexico: Generation Challenge programme, 2008. p. 23-25.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 51. | | HUFNAGEL, B.; SOUSA, S. M. de; ASSIS, L.; GUIMARAES, C. T.; LEISER, W.; AZEVEDO, G. C.; NEGRI, B.; LARSON, B. G.; SHAFF, J. E.; PASTINA, M. M.; BARROS, B. A.; WELTZIEN, E.; RATTUNDE, H. F. W.; VIANA, J. H.; CLARK, R. T.; FALCÃO, A.; GAZAFFI, R.; GARCIA, A. A. F.; SCHAFFERT, R. E.; KOCHIAN, L. V.; MAGALHAES, J. V. Duplicate and conquer: multiple homologs of PHOSPHORUS-STARVATION TOLERANCE1 enhance phosphorus acquisition and sorghum performance on low-phosphorus soils. Plant Physiology, Bethesda, v. 166, p. 659-677, Oct. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 52. | | MELO, J. O.; MARTINS, L. G. C.; BARROS, B. de A.; PIMENTA, M. R.; LANA, U. G. de P.; DUARTE, C. E. M.; PASTINA, M. M.; GUIMARÃES, C. T.; SCHAFFERT, R. E.; KOCHIAN, L. V.; FONTES, E. P. B.; MAGALHAES, J. V. de. Repeat variants for the SbMATE transporter protect sorghum roots from aluminum toxicity by transcriptional interplay in cis and trans. Proceedings of the National Academy of Sciences of the United States of America, Washington, v. 116, n. 1, p. 313-318, 2019. Publicado online em 13 dez. 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 53. | | CANIATO, F. F.; GUIMARAES, C. T.; HAMBLIN, M.; BILLOT, C.; RAMI, J.-F.; HUFNAGEL, B.; KOCHIAN, L. V.; LIU, J.; GARCIA, A. A. F.; HASH, C. T.; RAMU, P.; MITCHELL, S.; KRESIVICH, S.; OLIVEIRA, A. C. de; AVELLAR, G. de; BORÉM, A.; GLASZMANN, J.-C.; SCHAFFERT, R. E.; MAGALHAES, J. V. The relationship between population structure and aluminum tolerance in cultivated sorghum. Plos One, San Francisco, v. 6, n. 6, Jun. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 53 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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