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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
09/01/2019 |
Data da última atualização: |
24/01/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MELO, J. O.; MARTINS, L. G. C.; BARROS, B. de A.; PIMENTA, M. R.; LANA, U. G. de P.; DUARTE, C. E. M.; PASTINA, M. M.; GUIMARÃES, C. T.; SCHAFFERT, R. E.; KOCHIAN, L. V.; FONTES, E. P. B.; MAGALHAES, J. V. de. |
Afiliação: |
Janaina O. Melo, Universidade Federal de Minas Gerais; Laura G. C. Martins, Universidade Federal de Viçosa; BEATRIZ DE ALMEIDA BARROS, CNPMS; Maiana R. Pimenta, Universidade Federal de Viçosa; UBIRACI GOMES DE PAULA LANA, CNPMS; Christiane E. M. Duarte, Universidade Federal de Viçosa; MARIA MARTA PASTINA, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; ROBERT EUGENE SCHAFFERT, CNPMS; Leon V. Kochian, University of Saskatchewan, Canada; Elizabeth P. B. Fontes, Universidade Federal de Viçosa; JURANDIR VIEIRA DE MAGALHAES, CNPMS. |
Título: |
Repeat variants for the SbMATE transporter protect sorghum roots from aluminum toxicity by transcriptional interplay in cis and trans. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Proceedings of the National Academy of Sciences of the United States of America, Washington, v. 116, n. 1, p. 313-318, 2019. |
DOI: |
10.1073/pnas.1808400115 |
Idioma: |
Inglês |
Notas: |
Publicado online em 13 dez. 2018. |
Conteúdo: |
Acidic soils, where aluminum (Al) toxicity is a major agricultural constraint, are globally widespreadand are prevalent in developing countries. In sorghum, the root citrate transporter SbMATE confers Al tolerance by protecting root apices from toxic Al3+, but can exhibit reduced expression when introgressed into different lines. We show that allele-specific SbMATE transactivation occurs and is caused by factors located away from SbMATE. Using expressionQTL mapping and expression genome-wide association mapping, we establish that SbMATE transcription is controlled in a bipartite fashion,primarily incis butalsointrans.Multiallelicpromotertransactivation and ChIP analyses demonstrated that intermolecular effects on SbMATE expression arise from a WRKY and a zinc fingerDHHC transcription factor (TF) that bind to and trans-activate the SbMATE promoter. A haplotype analysis in sorghum RILs indicates that the TFs influence SbMATE expression and Al tolerance. Variation in SbMATE expression likely results from changes in tandemly repeated cis sequences flanking a transposable element (a miniature inverted repeattransposable element)insertion inthe SbMATE promoter, which are recognized by the Al3+-responsive TFs. According to our model, repeat expansion in Al-tolerant genotypes increases TF recruitment and, hence, SbMATE expression, which is, in turn, lower in Al-sensitive genetic backgrounds as a result of lower TF expression and fewer binding sites. We thus show that even dominant cis regulation of an agronomically important gene can be subjected to precise intermolecular finetuning. These concerted cis/trans interactions, which allow the plant to sense and respond to environmental cues, such as Al3+ toxicity, can now be used to increase yields and food security on acidic soils. MenosAcidic soils, where aluminum (Al) toxicity is a major agricultural constraint, are globally widespreadand are prevalent in developing countries. In sorghum, the root citrate transporter SbMATE confers Al tolerance by protecting root apices from toxic Al3+, but can exhibit reduced expression when introgressed into different lines. We show that allele-specific SbMATE transactivation occurs and is caused by factors located away from SbMATE. Using expressionQTL mapping and expression genome-wide association mapping, we establish that SbMATE transcription is controlled in a bipartite fashion,primarily incis butalsointrans.Multiallelicpromotertransactivation and ChIP analyses demonstrated that intermolecular effects on SbMATE expression arise from a WRKY and a zinc fingerDHHC transcription factor (TF) that bind to and trans-activate the SbMATE promoter. A haplotype analysis in sorghum RILs indicates that the TFs influence SbMATE expression and Al tolerance. Variation in SbMATE expression likely results from changes in tandemly repeated cis sequences flanking a transposable element (a miniature inverted repeattransposable element)insertion inthe SbMATE promoter, which are recognized by the Al3+-responsive TFs. According to our model, repeat expansion in Al-tolerant genotypes increases TF recruitment and, hence, SbMATE expression, which is, in turn, lower in Al-sensitive genetic backgrounds as a result of lower TF expression and fewer binding sites. We thus show that even dominant c... Mostrar Tudo |
Palavras-Chave: |
Estresse abiótico; Regulação transcricional; Toxicidade do alumínio. |
Thesagro: |
Gene; Genética. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 02927naa a2200337 a 4500 001 2103451 005 2020-01-24 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1073/pnas.1808400115$2DOI 100 1 $aMELO, J. O. 245 $aRepeat variants for the SbMATE transporter protect sorghum roots from aluminum toxicity by transcriptional interplay in cis and trans.$h[electronic resource] 260 $c2019 500 $aPublicado online em 13 dez. 2018. 520 $aAcidic soils, where aluminum (Al) toxicity is a major agricultural constraint, are globally widespreadand are prevalent in developing countries. In sorghum, the root citrate transporter SbMATE confers Al tolerance by protecting root apices from toxic Al3+, but can exhibit reduced expression when introgressed into different lines. We show that allele-specific SbMATE transactivation occurs and is caused by factors located away from SbMATE. Using expressionQTL mapping and expression genome-wide association mapping, we establish that SbMATE transcription is controlled in a bipartite fashion,primarily incis butalsointrans.Multiallelicpromotertransactivation and ChIP analyses demonstrated that intermolecular effects on SbMATE expression arise from a WRKY and a zinc fingerDHHC transcription factor (TF) that bind to and trans-activate the SbMATE promoter. A haplotype analysis in sorghum RILs indicates that the TFs influence SbMATE expression and Al tolerance. Variation in SbMATE expression likely results from changes in tandemly repeated cis sequences flanking a transposable element (a miniature inverted repeattransposable element)insertion inthe SbMATE promoter, which are recognized by the Al3+-responsive TFs. According to our model, repeat expansion in Al-tolerant genotypes increases TF recruitment and, hence, SbMATE expression, which is, in turn, lower in Al-sensitive genetic backgrounds as a result of lower TF expression and fewer binding sites. We thus show that even dominant cis regulation of an agronomically important gene can be subjected to precise intermolecular finetuning. These concerted cis/trans interactions, which allow the plant to sense and respond to environmental cues, such as Al3+ toxicity, can now be used to increase yields and food security on acidic soils. 650 $aGene 650 $aGenética 653 $aEstresse abiótico 653 $aRegulação transcricional 653 $aToxicidade do alumínio 700 1 $aMARTINS, L. G. C. 700 1 $aBARROS, B. de A. 700 1 $aPIMENTA, M. R. 700 1 $aLANA, U. G. de P. 700 1 $aDUARTE, C. E. M. 700 1 $aPASTINA, M. M. 700 1 $aGUIMARÃES, C. T. 700 1 $aSCHAFFERT, R. E. 700 1 $aKOCHIAN, L. V. 700 1 $aFONTES, E. P. B. 700 1 $aMAGALHAES, J. V. de 773 $tProceedings of the National Academy of Sciences of the United States of America, Washington$gv. 116, n. 1, p. 313-318, 2019.
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Embrapa Milho e Sorgo (CNPMS) |
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Registros recuperados : 68 | |
2. | | CANIATO, F. F.; GUIMARAES, C. T.; SCHAFFERT, R. E.; KOCHIAN, L. V.; MAGALHAES, J. V. D. Association mapping for aluminum tolerance in candidate regions of the Altsb locus in sorghum. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 150.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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4. | | PINEROS, M. A.; SHAFF, J. E.; MANSLANK, H. S.; ALVES, V. M. C.; KOCHIAN, L. V. Aluminum resistance in maize cannot be solely explained by root organic acid exudation. A comparative physiological study Plant Physiology, Bethesda, v. 137, n. 1, p. 231-241, 2005.Tipo: Artigo em Periódico Indexado |
Biblioteca(s): Embrapa Milho e Sorgo. |
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10. | | MAGALHAES, J. V.; SOUSA, S. M. de; GUIMARAES, C. T.; KOCHIAN, L. V. The role of root morphology and architecture in phosphorus acquisiton: physiological, genetic, and molecular basis. In: HOSSAIN, M. A.; KAMIYA, T.; BURRITT, D. J.; PHAN TRAN, L.-S.; FUJIWARA, T. (Ed.). Plant macronutrient use efficiency: molecular and genomic perspectives in crop plants. London: Academic Press, 2017. cap. 7, p. 123-147.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Milho e Sorgo. |
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12. | | ALVES, V. M. C.; MAGALHAES, J. V. de; KOCHIAN, L. V.; PARENTONI, S. N.; PAIVA, E.; GARVIN, D. F. Acumulação de alumínio em ápices de raízes de milho. In: REUNIAO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRICAO DE PLANTAS, 24.; REUNIAO BRASILEIRA SOBRE MICORRIZAS, 8.; SIMPOSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 6.; REUNIAO BRASILEIRA DE BIOLOGIA DO SOLO, 3., 2000, Santa Maria, RS. Biodinâmica do solo: guia do congressista. [Viçosa, MG]: Sociedade Brasileira de Ciência do Solo; [São Paulo]: Sociedade Brasileira de Microbiologia; Santa Maria, RS: Universidade Federal de Santa Maria, Departamento de Solos, 2000. Fertbio 2000. p. 151.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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13. | | CANIATO, F. F.; HAMBLIN, M. T.; GUIMARAES, C. T.; ZHANG, Z.; SCHAFFERT, R. E.; KOCHIAN, L. V.; MAGALHAES, J. V. Association mapping provides insights into the origin and the fine structure of the sorghum aluminum tolerance locus, AltSB. Plos One, San Francisco, v. 9, n. 1, p. 1-12, 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Milho e Sorgo. |
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14. | | KOCHIAN, L.; HOEKENGA, O.; MAGALHAES, J.; PINEROS, M.; ALVES, V.; MARON, L.; MASON, P.; GUIMARAES, C.; SCHAFFERT, R. Integrating genomic, molecular genetic and physiological approaches to identify plant aluminum tolerance genes and their associated physilogical mechanisms. In: INTERNATIONAL PLANT NUTRITION COLLOQUIUM, 15., 2005, Beijing, China. Proceedings... Beijing: Tsinghua University, 2005. p. 18-19.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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15. | | LIU, J.; LUO, X.; SHAFF, J.; LIANG, C.; JIA, X.; LI, Z.; MAGALHAES, J.; KOCHIAN, L. V. A promoter-swap strategy between the AtALMT and AtMATE genes increased Arabidopsis aluminum resistance and improved carbon-use efficiency for aluminum resistance. The Plant Journal, Oxford, v. 71, p. 327-337, 2012.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Milho e Sorgo. |
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16. | | HUFNAGEL, B.; GUIMARÃES, C. T.; CRAFT, E. J.; SHAFF, J. E.; SCHAFFERT, R. E.; KOCHIAN, L. V.; MAGALHAES, J. V. Exploiting sorghum genetic diversity for enhanced aluminum tolerance: allele mining based on the AltSB locus. Scientific Reports, v. 8, p. 1-13, July 2018. Article number: 10094.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Milho e Sorgo. |
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17. | | ALVES, V. M. C.; MAGALHAES, J. V. de; SHAFF, J.; SCHAFFERT, R. E.; GUIMARAES, C. T.; KOCHIAN, L. V. Exsudação de citrato e tolerânica ao alumínio em sorgo. In: CONGRESSO NACIONAL DE MILHO E SORGO, 26.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 2.; SIMPÓSIO SOBRE COLLETOTRICHUM GRAMINICOLA, 1., 2006, Belo Horizonte. Inovação para sistemas integrados de produção: trabalhos apresentados. [Sete Lagoas]: ABMS, 2006. 1 CD-ROM.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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18. | | KOCHIAN, L.; MAGALHAES, J.; LIU, J.; HOEKENGA, O.; PINEROS, M.; SCHAFFERT, R. E.; SHAFF, J.; ALVES, V. Molecular approaches to rhizosphere research: identification and characterization of aluminum tolerance genes and their use to improve acid soil tolerance. In: INTERNATIONAL CONFERENCE RHIZOSPHERE , 2., 2007, Montpellier. Book of abstracts... Montpellier: INRA, 2007.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Milho e Sorgo. |
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19. | | ALVES, V. M. C.; GUIMARAES, C. T.; SHAFF, J.; KOCHIAN, L.; PARENTONI, S. N.; SCHAFFERT, R. E.; MAGALHÃES, J. Molecular and physiological analysis of aluminum tolerance in maize recombinant inbreds. In: INTERNATIONAL PLANT NUTRITION COLLOQUIUM, 16., 2009, Sacramento, California. Proceedings... Davis: University of California, 2009.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Milho e Sorgo. |
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20. | | PAPERNIK, L. A.; BETHEA, A. S.; SINGLETON, T. E.; MAGALHAES, J. V.; GARVIN, D. F.; KOCHIAN, L. V. Physiological basis of reduced Al tolerance in ditelosomic lines of Chinese spring wheat. Planta, New York, v. 212, n. 5/6, p. 829-834, 2001.Tipo: Artigo em Periódico Indexado |
Biblioteca(s): Embrapa Milho e Sorgo. |
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Registros recuperados : 68 | |
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