Data/hora: 23/09/2024 12:14:52

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	22/11/1999	
Data da última atualização: 	22/11/1999	
Autoria: 	KOBAYASHI, S.; UCHIMIYA, H.	
Afiliação: 	Akitsu Branch, Fruit Tree Research Station, Akitsu, Hiroshima 729-24; Institute of Biological Sciences, University of Tsukuba, Tsukuba City, Ibaraki 305.	
Título: 	Expression and integration of a foreign gene in orange (Citrus sinensis Osb.) protoplasts by direct DNA transfer	
Ano de publicação: 	1989	
Fonte/Imprenta: 	Jpn. J. Genet, v.64, p.91-97, 1989	
Idioma: 	Inglês	
Conteúdo: 	Protoplasts isolated from suspension cultured cells of orange (Citrus sinensis Osb.) were treated with a bacterial plasmid DNA carrying a chimeric gene consisting of the nopaline synthase promoter, the aminoglycoside phosphotrans-ferase II [APH(3) II] structural gene from the bacterial transposon Tn 5 and a terminator region from cauliflower mosaic virus DNA. Colonies capable of proliferating in a medium containing kanamycin (25 ug/ml) possessed APH (3) II activity and an intact foreign gene. The transformation frequency was recorded to be in the order of 10-6.	
Palavras-Chave: 	Citrus sinensis Osb; Cultur de tecidos; Hibridos somaticos; Orange; Protoplastos; Somatic hydrid; Tissus culture.	
Thesagro: 	Laranja.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (17428-1) - ADD - SP - -- - S11630 -  - S11630 - DIS