Data/hora: 23/09/2024 12:14:21

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	28/04/1995	
Data da última atualização: 	17/05/2023	
Autoria: 	KOBAYASHI, S.; OHGAWARA, T.; OHGAWARA, E.; OIYAMA, I.; ISHII, S.	
Afiliação: 	Akitsu Branch, Fruit Tree Rsearch Station, Akitsu, Hiroshima, Japao; Research & Development Division, Kikkoman Corporation, Noda-shi, China.	
Título: 	A somatic hybrid plant obtained by protoplast fusion between navel orange (Citrus sinensis) and satsuma mandarin (C. unshiu).	
Ano de publicação: 	1988	
Fonte/Imprenta: 	Plant Cell, tissue and Organ Culture, v. 14, p.63-69 1988.	
Idioma: 	Inglês	
Conteúdo: 	A somatic hybrid plant was obtained by protoplast fusion between navelorange and satsuma mandarin. Protoplasts isolated from nucellar calli of navel orange and from leaves of satsuma mandari were fused by the PEG method. The fusion products were cultured in a Murashige & Tucker medium containing 0.6M sucrose. In this medium, some colonies developedinto whole plants thorough embryogenesis. One of the regenerated plants was shown to be a hybrid, which was proven by restriction endonuclease analysis of nuclear ribosomal DNA. The chromosome number of the hybrid was 36. Both parents have a chromosome number 2n = 18.	
Palavras-Chave: 	rDNA analysis; Somatic hybrid plant.	
Thesaurus Nal: 	Citrus; protoplast fusion.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (15490-1) - ADC - AP - DD - Publicação digital -  -  - DIS