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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Milho e Sorgo. |
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Data corrente: |
10/07/2017 |
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Data da última atualização: |
24/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RIBEIRO, A. P.; SOUZA, W. R. de; MARTINS, P. K.; VINECKY, F.; DUARTE, K. E.; BASSO, M. F.; DIAS, B. B. A.; CAMPANHA, R. B.; OLIVEIRA, P. A. de; CENTENO, D. C.; CANÇADO, G. M. de A.; MAGALHÃES, J. V. de; SOUSA, C. A. F. de; ANDRADE, A. C.; KOBAYASHI, A. K.; MOLINARI, H. B. C. |
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Afiliação: |
WAGNER R. DE SOUZA, UFLA; POLYANA K. MARTINS, UFLA; FELIPE VINECKY, UFLA; KAROLINE E. DUARTE, UFLA; MARCOS F. BASSO, UFLA; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; RAQUEL BOMBARDA CAMPANHA, CNPAE; PATRICIA ABRAO DE OLIVEIRA, CNPAE; DANILO C. CENTENO, UFABC; GERALDO MAGELA DE ALMEIDA CANCADO, CNPTIA; JURANDIR VIEIRA DE MAGALHAES, CNPMS; CARLOS A. F. DE SOUSA, UFLA; ALAN CARVALHO ANDRADE, SAPC; ADILSON KENJI KOBAYASHI, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE. |
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Título: |
Overexpression of BdMATE gene improves aluminum tolerance in Setaria viridis. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 8, p. 1-12, June 2017. |
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Páginas: |
12 p. |
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DOI: |
10.3389/fpls.2017.00865 |
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Idioma: |
Inglês |
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Conteúdo: |
Acidic soils are distributed worldwide, predominantly in tropical and subtropical areas,reaching around 50% of the arable soil. This type of soil strongly reduces crop production, mainly because of the presence of aluminum, which has its solubility increased at low pH levels. A well-known physiological mechanism used by plants to cope with Al stress involves activation of membrane transporters responsible for organic acid anions secretion from the root apex to the rhizosphere, which chelate Al, preventing its absorption by roots. In sorghum, a membrane transporter gene belonging to multidrug and toxic compound extrusion (MATE) family was identified and characterized as an aluminum-activated citrate transporter gene responsible for Al tolerance in this crop. Setaria viridis is an emerging model for C4 species and it is an important model to validate some genes for further C4 crops transformation, such as sugarcane, maize, and wheat. In the present work, Setaria viridis was used as a model plant to overexpress a newly identified MATE gene from Brachypodium distachyon(BdMATE), closely related to SbMATE, for aluminum tolerance assays. Transgenic S. viridis plants overexpressing a BdMATE presented an improved Al tolerance phenotype, characterized by sustained root growth and exclusion of aluminum from the root apex in transgenic plants, as confirmed by hematoxylin assay. In addition, transgenic plants showed higher root citrate exudation into the rhizosphere, suggesting that Al tolerance improvement in these plants could be related to the chelation of the metal by the organic acid anion. These results suggest that BdMATE gene can be used to transform C4 crops of economic importance with improved aluminum tolerance. MenosAcidic soils are distributed worldwide, predominantly in tropical and subtropical areas,reaching around 50% of the arable soil. This type of soil strongly reduces crop production, mainly because of the presence of aluminum, which has its solubility increased at low pH levels. A well-known physiological mechanism used by plants to cope with Al stress involves activation of membrane transporters responsible for organic acid anions secretion from the root apex to the rhizosphere, which chelate Al, preventing its absorption by roots. In sorghum, a membrane transporter gene belonging to multidrug and toxic compound extrusion (MATE) family was identified and characterized as an aluminum-activated citrate transporter gene responsible for Al tolerance in this crop. Setaria viridis is an emerging model for C4 species and it is an important model to validate some genes for further C4 crops transformation, such as sugarcane, maize, and wheat. In the present work, Setaria viridis was used as a model plant to overexpress a newly identified MATE gene from Brachypodium distachyon(BdMATE), closely related to SbMATE, for aluminum tolerance assays. Transgenic S. viridis plants overexpressing a BdMATE presented an improved Al tolerance phenotype, characterized by sustained root growth and exclusion of aluminum from the root apex in transgenic plants, as confirmed by hematoxylin assay. In addition, transgenic plants showed higher root citrate exudation into the rhizosphere, suggesting that Al t... Mostrar Tudo |
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Palavras-Chave: |
BdMate; Hydroponic system; Organismo geneticamente modificado; Tolerância ao alumínio. |
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Thesagro: |
Aluminio. |
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Thesaurus Nal: |
Abiotic stress; Aluminum; Genetically modified organisms; Setaria viridis. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02981naa a2200433 a 4500
001 2080632
005 2018-01-24
008 2017 bl uuuu u00u1 u #d
024 7 $a10.3389/fpls.2017.00865$2DOI
100 1 $aRIBEIRO, A. P.
245 $aOverexpression of BdMATE gene improves aluminum tolerance in Setaria viridis.$h[electronic resource]
260 $c2017
300 $a12 p.
520 $aAcidic soils are distributed worldwide, predominantly in tropical and subtropical areas,reaching around 50% of the arable soil. This type of soil strongly reduces crop production, mainly because of the presence of aluminum, which has its solubility increased at low pH levels. A well-known physiological mechanism used by plants to cope with Al stress involves activation of membrane transporters responsible for organic acid anions secretion from the root apex to the rhizosphere, which chelate Al, preventing its absorption by roots. In sorghum, a membrane transporter gene belonging to multidrug and toxic compound extrusion (MATE) family was identified and characterized as an aluminum-activated citrate transporter gene responsible for Al tolerance in this crop. Setaria viridis is an emerging model for C4 species and it is an important model to validate some genes for further C4 crops transformation, such as sugarcane, maize, and wheat. In the present work, Setaria viridis was used as a model plant to overexpress a newly identified MATE gene from Brachypodium distachyon(BdMATE), closely related to SbMATE, for aluminum tolerance assays. Transgenic S. viridis plants overexpressing a BdMATE presented an improved Al tolerance phenotype, characterized by sustained root growth and exclusion of aluminum from the root apex in transgenic plants, as confirmed by hematoxylin assay. In addition, transgenic plants showed higher root citrate exudation into the rhizosphere, suggesting that Al tolerance improvement in these plants could be related to the chelation of the metal by the organic acid anion. These results suggest that BdMATE gene can be used to transform C4 crops of economic importance with improved aluminum tolerance.
650 $aAbiotic stress
650 $aAluminum
650 $aGenetically modified organisms
650 $aSetaria viridis
650 $aAluminio
653 $aBdMate
653 $aHydroponic system
653 $aOrganismo geneticamente modificado
653 $aTolerância ao alumínio
700 1 $aSOUZA, W. R. de
700 1 $aMARTINS, P. K.
700 1 $aVINECKY, F.
700 1 $aDUARTE, K. E.
700 1 $aBASSO, M. F.
700 1 $aDIAS, B. B. A.
700 1 $aCAMPANHA, R. B.
700 1 $aOLIVEIRA, P. A. de
700 1 $aCENTENO, D. C.
700 1 $aCANÇADO, G. M. de A.
700 1 $aMAGALHÃES, J. V. de
700 1 $aSOUSA, C. A. F. de
700 1 $aANDRADE, A. C.
700 1 $aKOBAYASHI, A. K.
700 1 $aMOLINARI, H. B. C.
773 $tFrontiers in Plant Science$gv. 8, p. 1-12, June 2017.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 104 | |
| 41. |  | RAPOSO, R. da S.; BRITTO, F. B.; SOUZA, I. G. de B; CARVALHO, A. M. F. de; KOBAYASHI, A. K.; LAVIOLA, B. G.; BENTZEN, P.; DINIZ, F. M. Development and use of novel microsatellite markers from double-enriched genomic libraries in Guatemalan Jatropha curcas. Biochemical Systematics and Ecology, v. 59, p. 168-173, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroenergia; Embrapa Meio-Norte. |
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| 42. | | KOBAYASHI, A. K.; DIAZ TRUJILLO, C.; LEE, T. van der; ZWIERS, L.-H.; PAIVA, L. V.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. Generation of transgenic Mycosphaerella fijiensis expressing reporter genes: tools for pathogenicity and mating studies. In: INTERNATIONAL MYCOSPHAERELLA AND STAGONOSPORA SYMPOSIUM, 7., 2008. Ascona, Switzerland. Program, Presentations, Abstracts. Ascona, Switzerland, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 43. | | SANTOS, T. T.; CASARI, R. A. C. N.; OLIVEIRA, N. G.; DIAS, B. B. A.; KOBAYASHI, A. K.; MOLINARI, H. B. C.; SOUSA, C. A. F. de. A protocol for measuring canopy temperature of sugarcane plots in the field using a thermal imager embarked in an UAV. In: LATIN-AMERICAN CONFERENCE ON PLANT PHENOTYPING AND PHENOMICS FOR PLANT BREEDING, 2., 2017, São Carlos, SP. Proceedings... São Carlos: Embrapa Instrumentação, 2017. p. 26.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 44. | | SANTOS, T. T.; CASARI, R. A. C. N.; OLIVEIRA, N. G.; DIAS, B. B. A.; KOBAYASHI, A. K.; MOLINARI, H. B. C.; SOUSA, C. A. F. de. A protocol for measuring canopy temperature of sugarcane plots in the field using a thermal imager embarked in an UAV. In: LATIN-AMERICAN CONFERENCE ON PLANT PHENOTYPING AND PHENOMICS FOR PLANT BREEDING, 2., 2017, São Carlos, SP. Proceedings... São Carlos: Embrapa Instrumentação, 2017. p. 26.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 45. | | LAVIOLA, B. G.; BHERING, L. L.; MOLINARI, H. B. C.; DURAES, F. O. M.; ALBRECHT, J. C.; KOBAYASHI, A. K.; ARRIEL, N. H. C. Recursos genéticos de pinhão manso para a produção de óleos e produtos da bioenergia no Brasil. In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais...Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. p. 137| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 46. | | LAVIOLA, B. G.; BHERING, L. L.; QUIRINO, B. F.; MOLINARI, H. B. C.; DURAES, F. O. M.; ALBRECHT, J. C.; KOBAYASHI, A. K.; ARRIEL, N. H. C. Recursos genéticos de pinhão manso para a produção de óleos e produtos da bioenergia no Brasil. In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. p. 387.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 47. | | LAVIOLA, B. G.; BHERING, L. L.; QUIRINO, B.; MOLINARI, H. B. C.; DURÃES, F. O. M.; ALBRECHT, J. C.; KOBAYASHI, A. K.; ARRIEL, N. H. C. Recursos genéticos de pinhão manso para a produção de óleos e produtos da bioenergia no Brasil. In: SIMPÓSIO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2008, Brasília, DF. Anais... Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. p. 387| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Algodão. |
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| 48. | | KOBAYASHI, A. K.; VIEIRA, L. G. E.; BESPALHOK FILHO, J. C.; LEITE JUNIOR, R. P.; PEREIRA, L. F. P.; MOLINARI, H. B. C.; MARQUES, V. V. Enhanced resistance to citrus canker in transgenic sweet orange expressing the sarcotoxin IA gene. European Journal of Plant Pathology, v. 149, n. 4, p. 865-873, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroenergia; Embrapa Café. |
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| 49. | | MARTINS, P. K.; DIAS, B. B. A.; RIBEIRO, A. P.; SOUTO, B. de M.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Isolation, in silico analysis and transformation of Setaria viridis with a sugarcane senescence-associated promoter. In: INTERNATIONAL SERTARIA GENETICS CONFERENCE, 1., 2014, Beijing, China. [Beijing]: China Agricultural Research Systems, 2014. p. 25.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 50. | | DIAS, B. B. A.; KOBAYASHI, A. K.; GOMES, A. de P. G.; RODRIGUES, D. de S.; MOLINARI, H. B. C.; FAVARO, L. C. de L.; MARTINS, P. K. Manual de Biossegurança da Embrapa Agroenergia (CQB 345/12). Brasília, DF: Embrapa Agroenergia, 2015. 35 p. ( Embrapa Agroenergia. Documentos, 19).| Biblioteca(s): Embrapa Agroenergia. |
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| 51. | | SOUSA, C. A. F. de; DIAS, B. B. A.; MARTINS, P. K.; MOLINARI, H. B. C.; KOBAYASHI, A. K.; SOUZA JUNIOR, M. T. Nova abordagem para a fenotipagem de plantas: conceitos, ferramentas e perspectivas. Revista Brasileira de Geografia Física, v. 8, p. 660-672, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 52. | | FERREIRA, C. F.; ALVES, E.; PESTANA, K. N.; JUNGHANS, D. T.; KOBAYASHI, A. K.; SANTOS, V. de J.; SILVA, R. P.; SILVA, P. H.; SOARES, E.; FUKUDA, W. Molecular characterization of cassava (Manihot esculenta Crantz) with yellow-orange roots for beta-carotene improvement. Crop Breeding and Applied Biotechnology, Londrina, v. 8, n. 1, p. 23-29, mar. 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - C |
| Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Meio-Norte. |
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| 53. | | BASSO, M. F.; FERREIRA, P. C. G.; KOBAYASHI, A. K.; HARMON, F. G.; NEPOMUCENO, A. L.; MOLINARI, H. B. C.; GROSSI-DE-SA, M. F. MicroRNAs and new biotechnological tools for its modulation and improving stress tolerance in plants. Plant Biotechnology Journal, v. 17, p. 1482-1500, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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| 54. | | BRITTO, F. B.; SABÓIA, E. N.; KOBAYASHI, A. K.; LAVIOLA, B. G.; ROSADO-LAVIOLA, T. B.; BENTZEN, P.; DINIZ, F. M. Molecular markers for the assessment of genetic diversity in Jatropha curcas L. In: CONGRESSO BRASILEIRO DE PESQUISA EM PINHÃO MANSO, 1., 2009, Brasília, DF. Anais... Brasília, DF: Embrapa Agroenergia; São Paulo: ABPPM, 2009. Editores: Bruno Galveas Laviola, Sérgio Delmar dos Anjos e Silva, Júlio César Albrecht. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Agroenergia. |
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| 55. | | Ribeiro, A. P.; Martins, P. K.; DIAS, B. B. A.; ANDRADE, A. C.; MAGALHAES, J. V. de; KOBAYASHI, A. K.; MOLINARI, H. B. C. MATE: a promising gene for aluminum tolerance in sugarcane. In: GERMPLASM AND BREEDING, 11.; MOLECULAR BIOLOGY ISSCT WORKSHOP, 8., 2015, Saint-Gilles Réunion Island. Pushing the frontiers of sugarcane improvement: abstract. [S.l]: Ercane, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 56. | | MOLINARI, H. B. C.; MARUR, C. J.; BESPALHOK FILHO, J. C.; KOBAYASHI, A. K.; PILEGGI, M.; LEITE JÚNIOR, R. P.; PEREIRA, L. F. P.; VIEIRA, L. G. E. Osmotic adjustment in transgenic citrus rootstock Carrizo citrange (Citrus sinensis Osb. x Poncirus trifoliata L. Raf.) overproducing proline. Plant Science, v. 167, n. 6, p. 1375-1381, 2004.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Café. |
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| 57. | | MARTINS, P. K.; NAKAYAMA, T. J.; RIBEIRO, A. P.; DIAS, B. B. A.; NEPOMUCENO, A. L.; HARMON, F. G.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis floral-dip: a simple and rapid Agrobacterium-mediated transformation method. Biotechnology Reports, v. 6, p. 61-63, 2015.| Biblioteca(s): Embrapa Agroenergia. |
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| 58. | | MARTINS, P. K.; MAFRA, V.; SOUZA, W. R. de; RIBEIRO, A. P.; VINECKY, F.; BASSO, M. F.; DIAS, B. B. A.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Selection of reliable reference genes for RT-qPCR analysis during developmental stages and abiotic stress in Setaria viridis. Scientific Reports, V. 6, 28348, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agroenergia. |
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| 59. | | MUDRY, C. S.; ALMEIDA, R. O.; DIAS, B. B. A.; KOBAYASHI, A. K.; BESPALHOK, F. J. C.; MOLINARI, H. B. C. Somatic embryogenesis in sugarcane cv. RB966928. In: CONGRESSO BRASILIEIRO DE BIOTECNOLOGIA, 4.,2012, Guaruja-SP, [ Anais...]./ Brasília, DF; CAPES: CNPq, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 60. | | DÍAZ-TRUJILLO, C.; KOBAYASHI, A. K.; SOUZA JUNIOR, M. T.; CHONG, P.; MEIJER, H. J. G.; ISAZA, R. E. A.; KEMA, G. H. J. Targeted and random genetic modification of the black Sigatoka pathogen Pseudocercospora fijiensis by Agrobacterium tumefaciens-mediated transformation. Journal of Microbiological Methods, v. 148, p. 127-137, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 104 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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