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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/04/2005 |
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Data da última atualização: |
01/02/2024 |
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Autoria: |
PADILLA, G.; KNIGHT, P. G.; HOLTZ, W. |
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Título: |
Superovulation of boer goats by active immunization against recombinant ovine inhibin. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Theriogenology, v. 53, n. 1, p. 506, 2000. |
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Idioma: |
Inglês |
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Notas: |
Edição de proceedings Annual Conference of the International Embryo Transfer Society, Maastricht, The Netherlands, jan. 2000. |
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Conteúdo: |
The objective of the study was the establishment of an alternative means of superovulating goats. During lhe estrous season 12 parous goats of the Boer goat breed received an im injection of 100 mg of the recombinant a subunit of ovine inhibin (r.oINH-a; Biotech Australia PTY. Ltd., RoseviIle Australia) in Montanide:Marcol (1 :9) adjuvant in oil emulsion. Another 12 animais were treated with lhe vehicle to serve as controls. The treatment was repeated 4 weeks later. Estrus was checked twice daily with an aproned buck, aod ftom lhe first day until 2 days after the last day of standing estrus, ovaries were inspected daily by ultrasonography using a 7.5 MHz transrectal linear transducer. Follicles of more than 4 mm in diameter were counted and measured with lhe scanner's electronic calipers. Ovulation was defined as the disappearence of follicles of more than 4 mm that had been present lhe day before. Blood samples were drawn at 2 week intervals to be assayed for inhibin antibody titer. The immune response was monitored by inhibin binding assar (RIA) in which samples were tested for the ability to bind 125I-labelled bovine inhibin (Mr 32 000). One year later, during the second estrus season, another booster injection was applied. AlI goats immunized with r.oINH-a produced antibodies. After the first immunization, titers rose from 2.9+ 0.4 (SEM) to 21.9+ 2.9 % and remained high for 10 weeks. In the second estrous season lhe response to lhe booster injection was more rapid and uniform. Binding levels were maintained for 11 weeks, though at a lower level of 11.7.t 0.4%. Only trace amounts of antibody < 2% binding) were detectable in control goats. In immunized goats the number of follicles (14.6+ 1.2 vs 2.5+ 0.1) and ovulations (6.9 + 0.7 vs 2.3+ 0.1) were increased over controls (P<0.01), SchefIé-test). The correlation of inhibin binding with number of follicles was r = 0.61 (P<0.01) and with number of ovulations, r = 0.27 (P<0.05). Short cycles <15 d) occurred more frequently in immunized goats in than controls (34 vs 6% of a total of 91 cycles; P<0.01, chi-square). Nonetheless it may be concluded that immunization against the r.oINH-a subunit may be a viable alternative to conventional superovulation treatments. MenosThe objective of the study was the establishment of an alternative means of superovulating goats. During lhe estrous season 12 parous goats of the Boer goat breed received an im injection of 100 mg of the recombinant a subunit of ovine inhibin (r.oINH-a; Biotech Australia PTY. Ltd., RoseviIle Australia) in Montanide:Marcol (1 :9) adjuvant in oil emulsion. Another 12 animais were treated with lhe vehicle to serve as controls. The treatment was repeated 4 weeks later. Estrus was checked twice daily with an aproned buck, aod ftom lhe first day until 2 days after the last day of standing estrus, ovaries were inspected daily by ultrasonography using a 7.5 MHz transrectal linear transducer. Follicles of more than 4 mm in diameter were counted and measured with lhe scanner's electronic calipers. Ovulation was defined as the disappearence of follicles of more than 4 mm that had been present lhe day before. Blood samples were drawn at 2 week intervals to be assayed for inhibin antibody titer. The immune response was monitored by inhibin binding assar (RIA) in which samples were tested for the ability to bind 125I-labelled bovine inhibin (Mr 32 000). One year later, during the second estrus season, another booster injection was applied. AlI goats immunized with r.oINH-a produced antibodies. After the first immunization, titers rose from 2.9+ 0.4 (SEM) to 21.9+ 2.9 % and remained high for 10 weeks. In the second estrous season lhe response to lhe booster injection was more rapid and un... Mostrar Tudo |
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Palavras-Chave: |
Imunidade ativa; Inibidor; Raça Boer. |
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Thesagro: |
Caprino; Endocrinologia; Reprodução Animal; Superovulação. |
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Categoria do assunto: |
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Marc: |
LEADER 02996nam a2200229 a 4500
001 1531051
005 2024-02-01
008 2000 bl uuuu u00u1 u #d
100 1 $aPADILLA, G.
245 $aSuperovulation of boer goats by active immunization against recombinant ovine inhibin.
260 $aTheriogenology, v. 53, n. 1, p. 506$c2000
500 $aEdição de proceedings Annual Conference of the International Embryo Transfer Society, Maastricht, The Netherlands, jan. 2000.
520 $aThe objective of the study was the establishment of an alternative means of superovulating goats. During lhe estrous season 12 parous goats of the Boer goat breed received an im injection of 100 mg of the recombinant a subunit of ovine inhibin (r.oINH-a; Biotech Australia PTY. Ltd., RoseviIle Australia) in Montanide:Marcol (1 :9) adjuvant in oil emulsion. Another 12 animais were treated with lhe vehicle to serve as controls. The treatment was repeated 4 weeks later. Estrus was checked twice daily with an aproned buck, aod ftom lhe first day until 2 days after the last day of standing estrus, ovaries were inspected daily by ultrasonography using a 7.5 MHz transrectal linear transducer. Follicles of more than 4 mm in diameter were counted and measured with lhe scanner's electronic calipers. Ovulation was defined as the disappearence of follicles of more than 4 mm that had been present lhe day before. Blood samples were drawn at 2 week intervals to be assayed for inhibin antibody titer. The immune response was monitored by inhibin binding assar (RIA) in which samples were tested for the ability to bind 125I-labelled bovine inhibin (Mr 32 000). One year later, during the second estrus season, another booster injection was applied. AlI goats immunized with r.oINH-a produced antibodies. After the first immunization, titers rose from 2.9+ 0.4 (SEM) to 21.9+ 2.9 % and remained high for 10 weeks. In the second estrous season lhe response to lhe booster injection was more rapid and uniform. Binding levels were maintained for 11 weeks, though at a lower level of 11.7.t 0.4%. Only trace amounts of antibody < 2% binding) were detectable in control goats. In immunized goats the number of follicles (14.6+ 1.2 vs 2.5+ 0.1) and ovulations (6.9 + 0.7 vs 2.3+ 0.1) were increased over controls (P<0.01), SchefIé-test). The correlation of inhibin binding with number of follicles was r = 0.61 (P<0.01) and with number of ovulations, r = 0.27 (P<0.05). Short cycles <15 d) occurred more frequently in immunized goats in than controls (34 vs 6% of a total of 91 cycles; P<0.01, chi-square). Nonetheless it may be concluded that immunization against the r.oINH-a subunit may be a viable alternative to conventional superovulation treatments.
650 $aCaprino
650 $aEndocrinologia
650 $aReprodução Animal
650 $aSuperovulação
653 $aImunidade ativa
653 $aInibidor
653 $aRaça Boer
700 1 $aKNIGHT, P. G.
700 1 $aHOLTZ, W.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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