|
|
Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
11/02/2016 |
Data da última atualização: |
12/02/2016 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
KMIT, M. C. P.; LIMA, A. O. S.; ROMAGNOLI, E. M.; ABDALLA, A. L.; MENDES, R. |
Afiliação: |
M. C. P. KMIT, ESALQ/USP; A. O. S. LIMA, UNIVALI; E. M. ROMAGNOLI, ESALQ/USP; A. L. ABDALLA, CENA; RODRIGO MENDES, CNPMA. |
Título: |
Sheep rumen shotgun sequencing for biomass-degrading genes discovery. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 1676-1. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: The lignocellulose present in the plant biomass, is a promising source of energy generation. However, the breakdown of plant biomass into simple sugars for bioethanol production is still inefficient and costly due to the recalcitrant nature of plant fiber. Sheep rumen microbiome is specialized in degradation of plant material, but most members of this complex community are non-cultivable in laboratory. Therefore, the search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to obtain plant biomass-degrading genes, selected from the sheep rumen microorganisms. The rumen samples were collected from 6 fistulated animals (Ovis aries), divided into two groups and subjected to two diets: control and sugarcane bagasse, 60 days after the beginning of the experiment. To characterize biomass-degrading genes, the metagenomic DNA was extracted from the solid contents of rumen followed by sequencing in MiSeq Personal Sequencer platform (Illumina®). We analyzed, 4,68 gigabases of metagenomic total DNA from microbes adherent to plant fiber, using on the CLC Genomic Workbench v.5.5.1 platform (CLC Bio, Denmark). The assembled contigs that allowed identification of 27 putative partial carbohydrate-active enzymes (CAE) (NCBI-nr) representing a total of 11 lignocellulases, 13 amylases and 3 other putative CAE from animals fed with control diet and 106 putative partial CAE representing a total of 52 lignocellulases, 46 amylases and 8 other putative CAE from animals fed with diet amended with sugarcane bagasse. These data sets shows the sheep rumen microbiome as an untapped source of potential new fibrolytic enzymes. Using a diet amended with sugarcane bagasse increases the abundance of CAE and provide a substantially expanded catalog of genes participating in the deconstruction of plant biomass MenosAbstract: The lignocellulose present in the plant biomass, is a promising source of energy generation. However, the breakdown of plant biomass into simple sugars for bioethanol production is still inefficient and costly due to the recalcitrant nature of plant fiber. Sheep rumen microbiome is specialized in degradation of plant material, but most members of this complex community are non-cultivable in laboratory. Therefore, the search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to obtain plant biomass-degrading genes, selected from the sheep rumen microorganisms. The rumen samples were collected from 6 fistulated animals (Ovis aries), divided into two groups and subjected to two diets: control and sugarcane bagasse, 60 days after the beginning of the experiment. To characterize biomass-degrading genes, the metagenomic DNA was extracted from the solid contents of rumen followed by sequencing in MiSeq Personal Sequencer platform (Illumina®). We analyzed, 4,68 gigabases of metagenomic total DNA from microbes adherent to plant fiber, using on the CLC Genomic Workbench v.5.5.1 platform (CLC Bio, Denmark). The assembled contigs that allowed identification of 27 putative partial carbohydrate-active enzymes (CAE) (NCBI-nr) representing a total of 11 lignocellulas... Mostrar Tudo |
Palavras-Chave: |
Lignocelluloytic enzymes; Microbial metagenome; Secind generation biofuel. |
Thesagro: |
Biotecnologia; Enzima. |
Thesaurus Nal: |
Metagenomics. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/138802/1/2015RA-065.pdf
|
Marc: |
LEADER 02806nam a2200229 a 4500 001 2036656 005 2016-02-12 008 2015 bl uuuu u00u1 u #d 100 1 $aKMIT, M. C. P. 245 $aSheep rumen shotgun sequencing for biomass-degrading genes discovery.$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 1676-1.$c1676 520 $aAbstract: The lignocellulose present in the plant biomass, is a promising source of energy generation. However, the breakdown of plant biomass into simple sugars for bioethanol production is still inefficient and costly due to the recalcitrant nature of plant fiber. Sheep rumen microbiome is specialized in degradation of plant material, but most members of this complex community are non-cultivable in laboratory. Therefore, the search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to obtain plant biomass-degrading genes, selected from the sheep rumen microorganisms. The rumen samples were collected from 6 fistulated animals (Ovis aries), divided into two groups and subjected to two diets: control and sugarcane bagasse, 60 days after the beginning of the experiment. To characterize biomass-degrading genes, the metagenomic DNA was extracted from the solid contents of rumen followed by sequencing in MiSeq Personal Sequencer platform (Illumina®). We analyzed, 4,68 gigabases of metagenomic total DNA from microbes adherent to plant fiber, using on the CLC Genomic Workbench v.5.5.1 platform (CLC Bio, Denmark). The assembled contigs that allowed identification of 27 putative partial carbohydrate-active enzymes (CAE) (NCBI-nr) representing a total of 11 lignocellulases, 13 amylases and 3 other putative CAE from animals fed with control diet and 106 putative partial CAE representing a total of 52 lignocellulases, 46 amylases and 8 other putative CAE from animals fed with diet amended with sugarcane bagasse. These data sets shows the sheep rumen microbiome as an untapped source of potential new fibrolytic enzymes. Using a diet amended with sugarcane bagasse increases the abundance of CAE and provide a substantially expanded catalog of genes participating in the deconstruction of plant biomass 650 $aMetagenomics 650 $aBiotecnologia 650 $aEnzima 653 $aLignocelluloytic enzymes 653 $aMicrobial metagenome 653 $aSecind generation biofuel 700 1 $aLIMA, A. O. S. 700 1 $aROMAGNOLI, E. M. 700 1 $aABDALLA, A. L. 700 1 $aMENDES, R.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Meio Ambiente (CNPMA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
12/11/2008 |
Data da última atualização: |
22/02/2019 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
SILVA, L. D. da; LIMA, M. S. de; LOPES, F. S. C.; GARZIERA, L.; PARANHOS, B. A. J.; SANCHES, N. F. |
Afiliação: |
Leonardo Dantas da Silva; Mauricio Silva de Lima, UFAL; Fabiana Soares Cariri Lopes; Luiza Garziera; BEATRIZ AGUIAR GIORDANO PARANHOS, CPATSA; Nilton Fritzons Sanches, CNPMF. |
Título: |
Período e viabilidade larval-adulto de Cryptolaemus montrouzieri (Mulsant) (Coleoptera: Coccinelidae) sobre Dactylopius opuntiae (Cockerell) (Hemiptera: Dactylopiidae). |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação : anais. Viçosa, MG: UFV, 2008. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Conteúdo: |
Este trabalho teve como objetivo verificar o período e a viabilidade larval-adulto de Cryptolaemus montrouzieri, espécie introduzida no país, tendo D. opuntiae como presa. |
Palavras-Chave: |
Cochonilha-do-carmim; Joaninha australiana; Manejo integrado; MIP. |
Thesagro: |
Controle Biológico; Palma Forrageira; Praga. |
Thesaurus NAL: |
Opuntia ficus-indica. |
Categoria do assunto: |
O Insetos e Entomologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPATSA-2009-09/39532/1/OPB2002.pdf
|
Marc: |
LEADER 01117nam a2200277 a 4500 001 1162884 005 2019-02-22 008 2008 bl uuuu u00u1 u #d 100 1 $aSILVA, L. D. da 245 $aPeríodo e viabilidade larval-adulto de Cryptolaemus montrouzieri (Mulsant) (Coleoptera$bCoccinelidae) sobre Dactylopius opuntiae (Cockerell) (Hemiptera: Dactylopiidae). 260 $aIn: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 22., 2008, Uberlândia. Ciência, tecnologia e inovação : anais. Viçosa, MG: UFV$c2008 300 $c1 CD-ROM. 520 $aEste trabalho teve como objetivo verificar o período e a viabilidade larval-adulto de Cryptolaemus montrouzieri, espécie introduzida no país, tendo D. opuntiae como presa. 650 $aOpuntia ficus-indica 650 $aControle Biológico 650 $aPalma Forrageira 650 $aPraga 653 $aCochonilha-do-carmim 653 $aJoaninha australiana 653 $aManejo integrado 653 $aMIP 700 1 $aLIMA, M. S. de 700 1 $aLOPES, F. S. C. 700 1 $aGARZIERA, L. 700 1 $aPARANHOS, B. A. J. 700 1 $aSANCHES, N. F.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Semiárido (CPATSA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|