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| Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
29/03/2005 |
Data da última atualização: |
16/05/2022 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
KLUTHCOUSKI, J.; STONE, L. F. |
Afiliação: |
JOAO KLUTHCOUSKI, CNPAF; LUIS FERNANDO STONE, CNPAF. |
Título: |
Desempenho de culturas anuais sobre palhada de braquiária. |
Ano de publicação: |
2003 |
Fonte/Imprenta: |
In: KLUTHCOUSKI, J.; STONE, L. F.; AIDAR, H. (Ed.). Integração lavoura-pecuária. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2003. |
Páginas: |
p. 499-521. |
ISBN: |
85-7437-018-5 |
Idioma: |
Português |
Conteúdo: |
Introdução. Palhadas para cobertura morta na região tropical. Influência da palhada de braquiária nas plantas daninhas. Rendimento do feijoeiro sobre palhada de braquiária. Rendimento da soja sobre palhada de braquiária. |
Palavras-Chave: |
Integração lavoura-pecuária; Planta daninha. |
Thesagro: |
Brachiaria; Cobertura Morta; Feijão; Palhada; Rendimento; Soja. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
Marc: |
LEADER 00972naa a2200253 a 4500 001 1213157 005 2022-05-16 008 2003 bl uuuu u00u1 u #d 020 $a85-7437-018-5 100 1 $aKLUTHCOUSKI, J. 245 $aDesempenho de culturas anuais sobre palhada de braquiária. 260 $c2003 300 $ap. 499-521. 520 $aIntrodução. Palhadas para cobertura morta na região tropical. Influência da palhada de braquiária nas plantas daninhas. Rendimento do feijoeiro sobre palhada de braquiária. Rendimento da soja sobre palhada de braquiária. 650 $aBrachiaria 650 $aCobertura Morta 650 $aFeijão 650 $aPalhada 650 $aRendimento 650 $aSoja 653 $aIntegração lavoura-pecuária 653 $aPlanta daninha 700 1 $aSTONE, L. F. 773 $tIn: KLUTHCOUSKI, J.; STONE, L. F.; AIDAR, H. (Ed.). Integração lavoura-pecuária. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2003.
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Embrapa Arroz e Feijão (CNPAF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
19/12/2018 |
Data da última atualização: |
29/04/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SILVA, P. R. A. da; VIDAL, M. S.; SOARES, C. de P.; POLESE, V.; TADRA-SFEIR, M. Z.; SOUZA, E. M. de; ARAUJO, J. L. S. de; BALDANI, J. I. |
Afiliação: |
PAULA RENATA ALVES DA SILVA, BOLSISTA EMBRAPA AGROBIOLOGIA; MARCIA SOARES VIDAL, CNPAB; CLEITON DE PAULA SOARES, UFRJ; VALÉRIA POLESE, UFRJ; MICHELLE ZIBETTI TADRA-SFEIR,, UFRJ; EMANUEL MALTEMPI DE SOUZA, UFRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; JOSE IVO BALDANI, CNPAB. |
Título: |
Sugarcane apoplast fluid modulates the global transcriptional profile of the diazotrophic bacteria Paraburkholderia tropica strain Ppe8. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
PLOS ONE, v. 13, n. 12, e0207863, 2018. |
ISSN: |
1932-6203 |
Idioma: |
Português |
Conteúdo: |
The stalk apoplast fluid of sugarcane contains different sugars, organic acids and amino acids that may supply the demand for carbohydrates by endophytic bacteria including diazotrophs P. tropica (syn. B. tropica) strain Ppe8, isolated from sugarcane, is part of the bacterial consortium recommended as inoculant to sugarcane. However, little information has been accumulated regarding this plant-bacterium interaction considering that it colonizes internal sugarcane tissues. Here, we made use of the RNA-Seq transcriptomic analysis to study the influence of sugarcane stalk apoplast fluid on Ppe8 gene expression. The bacterium was grown in JMV liquid medium (100 ml), divided equally and then supplemented with 50 ml of fresh JMV medium or 50 ml of apoplast fluid extracted from sugarcane variety RB867515. Total RNA was extracted 2 hours later, the rRNAs were depleted and mRNAs used to construct libraries to sequence the fragments using Ion Torrent technology. The mapping and statistical analysis were carried out with CLC Genomics Workbench software. The RNA-seq data was validated by RT-qPCR using the reference genes fliP1, paaF, and groL. The data analysis showed that 544 genes were repressed and 153 genes were induced in the presence of apoplast fluid. Genes that induce plant defense responses, genes related to chemotaxis and movements were repressed in the presence of apoplast fluid, indicating that strain Ppe8 recognizes the apoplast fluid as a plant component. The expression of genes involved in bacterial metabolism was regulated (up and down), suggesting that the metabolism of strain Ppe8 is modulated by the apoplast fluid. These results suggest that Ppe8 alters its gene expression pattern in the presence of apoplast fluid mainly in order to use compounds present in the fluid as well as to avoid the induction of plant defense mechanisms. This is a pioneer study showing the role played by the sugarcane apoplast fluid on the global modulation of genes in P. tropica strain Ppe8. MenosThe stalk apoplast fluid of sugarcane contains different sugars, organic acids and amino acids that may supply the demand for carbohydrates by endophytic bacteria including diazotrophs P. tropica (syn. B. tropica) strain Ppe8, isolated from sugarcane, is part of the bacterial consortium recommended as inoculant to sugarcane. However, little information has been accumulated regarding this plant-bacterium interaction considering that it colonizes internal sugarcane tissues. Here, we made use of the RNA-Seq transcriptomic analysis to study the influence of sugarcane stalk apoplast fluid on Ppe8 gene expression. The bacterium was grown in JMV liquid medium (100 ml), divided equally and then supplemented with 50 ml of fresh JMV medium or 50 ml of apoplast fluid extracted from sugarcane variety RB867515. Total RNA was extracted 2 hours later, the rRNAs were depleted and mRNAs used to construct libraries to sequence the fragments using Ion Torrent technology. The mapping and statistical analysis were carried out with CLC Genomics Workbench software. The RNA-seq data was validated by RT-qPCR using the reference genes fliP1, paaF, and groL. The data analysis showed that 544 genes were repressed and 153 genes were induced in the presence of apoplast fluid. Genes that induce plant defense responses, genes related to chemotaxis and movements were repressed in the presence of apoplast fluid, indicating that strain Ppe8 recognizes the apoplast fluid as a plant component. The expression of... Mostrar Tudo |
Palavras-Chave: |
Bacterial growth; RNA Sequence; Sugarcane variety RB867515; Transcriptomic profile. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02828naa a2200265 a 4500 001 2102134 005 2019-04-29 008 2018 bl uuuu u00u1 u #d 022 $a1932-6203 100 1 $aSILVA, P. R. A. da 245 $aSugarcane apoplast fluid modulates the global transcriptional profile of the diazotrophic bacteria Paraburkholderia tropica strain Ppe8.$h[electronic resource] 260 $c2018 520 $aThe stalk apoplast fluid of sugarcane contains different sugars, organic acids and amino acids that may supply the demand for carbohydrates by endophytic bacteria including diazotrophs P. tropica (syn. B. tropica) strain Ppe8, isolated from sugarcane, is part of the bacterial consortium recommended as inoculant to sugarcane. However, little information has been accumulated regarding this plant-bacterium interaction considering that it colonizes internal sugarcane tissues. Here, we made use of the RNA-Seq transcriptomic analysis to study the influence of sugarcane stalk apoplast fluid on Ppe8 gene expression. The bacterium was grown in JMV liquid medium (100 ml), divided equally and then supplemented with 50 ml of fresh JMV medium or 50 ml of apoplast fluid extracted from sugarcane variety RB867515. Total RNA was extracted 2 hours later, the rRNAs were depleted and mRNAs used to construct libraries to sequence the fragments using Ion Torrent technology. The mapping and statistical analysis were carried out with CLC Genomics Workbench software. The RNA-seq data was validated by RT-qPCR using the reference genes fliP1, paaF, and groL. The data analysis showed that 544 genes were repressed and 153 genes were induced in the presence of apoplast fluid. Genes that induce plant defense responses, genes related to chemotaxis and movements were repressed in the presence of apoplast fluid, indicating that strain Ppe8 recognizes the apoplast fluid as a plant component. The expression of genes involved in bacterial metabolism was regulated (up and down), suggesting that the metabolism of strain Ppe8 is modulated by the apoplast fluid. These results suggest that Ppe8 alters its gene expression pattern in the presence of apoplast fluid mainly in order to use compounds present in the fluid as well as to avoid the induction of plant defense mechanisms. This is a pioneer study showing the role played by the sugarcane apoplast fluid on the global modulation of genes in P. tropica strain Ppe8. 653 $aBacterial growth 653 $aRNA Sequence 653 $aSugarcane variety RB867515 653 $aTranscriptomic profile 700 1 $aVIDAL, M. S. 700 1 $aSOARES, C. de P. 700 1 $aPOLESE, V. 700 1 $aTADRA-SFEIR, M. Z. 700 1 $aSOUZA, E. M. de 700 1 $aARAUJO, J. L. S. de 700 1 $aBALDANI, J. I. 773 $tPLOS ONE$gv. 13, n. 12, e0207863, 2018.
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